Location: Room 42
System specifications
Confocal microscope system with a broad range of scan speeds suited for high-resolution morphological imaging, as well as multispectral imaging.
- 350-900 nm spectral detection range with 5 nm spectral resolution and sub-micron spatial resolution
- Suitable for most fluorophores from DAPI to Cy5
- Measuring up to five dimensions, including x, y, z, time and spectrum
- Capable of multi-dimensional fluorescence qualitative and quantitative measurement, FRET, FRAP, FLIP, FLAP and other photobleaching-related applications
- Suitable for imaging fixed specimen (No environmental chamber).
Internal Detectors
- Spectral detection channels: PMT1, PMT2, PMT3
- Brightfield transmission channel: PMT Trans
Multi-dimension capability: xyz, xzy, xt, xyt, xzt, xyzt, xy, xz, xyt, xyz
Stage
- Manual moving X, Y stage
- Motorized z stage (piezo-controlled) with 166.68 µm scanning range
Zoom range: 1x-32x
Pinhole size: 20 µm-600 µm
Contrast enhancement options: Brightfield, DIC and polarized
Software: Leica LAS AF, Version 1.7.0, for image acquisition and processing
Scanners
Conventional scanner: 10-1400 Hz (2-3 frames/sec at 512 x 512 pixels)
Internal detectors
Reflection/fluorescence mode (spectral detection range 400-800 nm)
- Spectral detection channels: PMT1, PMT2, PMT3
- Additional PMT in transmission mode
Lasers
| Type | Wavelenght (nm) | Dyes and use |
|---|---|---|
| UV | 405 | DAPI, Hoechst |
| Argon | 458, 476, 488, 496, 514 | CyanFP, GFP, Alexa488, CY2, YFP |
| HeNe | 543 | Alexa 546, Alexa 555 |
| HeNe | 633 | Cy5, Alexa 647 |
- An acousto-optical tunable filter (AOTF) controls the intensity of the visible excitation laser light.
- An acousto-optical beam splitter (AOBS) is used to split excitation light and emission light instead of filter blocks.
Microscope
Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage
Objectives
| Magnification | NA | Type | Immersion | Coverslip | WD (mm) |
|---|---|---|---|---|---|
| 20x | 0.5 | PL Fluotar UV optimised | Dry | – / 0.17 | 0.68 |
| 40x | 1.25 | HC PL Apo UV optimised | Oil | 0.17 | 0.65 |
| 63x | 1.4 | HC PL Apo UV optimised | Oil | 0.17 | 0.14 |
Fluorescent source
Leica EL6000External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours)
Fluorescence filter sets
| Name | Excitation filter | Emission filter | Dichroic |
|---|---|---|---|
| A | BP 340 – 380 nm | LP 425 nm | 400 nm |
| I3 | BP 450- 490 nm | LP 515 nm | 510 nm |
| N2.1 | BP 515-560 | LP 590 | 580 nm |
Power up and down procedure
- Please contact Alvaro Crevenna to schedule a session
- Report problems on the Log file
Book this resource online (intranet)