{"id":489,"date":"2025-07-22T10:29:09","date_gmt":"2025-07-22T10:29:09","guid":{"rendered":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/?page_id=489"},"modified":"2025-07-23T09:59:18","modified_gmt":"2025-07-23T09:59:18","slug":"nanolive-cx-a","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/equipment\/nanolive-cx-a\/","title":{"rendered":"NanoLive CX-A"},"content":{"rendered":"\n
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The CX-A is an holotomography microscope designed to perform automated imaging of samples in 96-wells plates or 35mm Petri dishes. It allows to measure the refractive index of samples with sub-cellular resolution.<\/p>\n\n\n\n

Thanks to the label-free nature of the technology and the stage-top incubation system, the CX-A is particularly suited for long-term live cell imaging.<\/p>\n\n\n\n

Pixel intensities are a quantitative measure and are proportional to the dry mass, therefore subcellular structures such as lipid droplets, mitochondria, nuclear vesicles can be resolved.<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

<\/p>\n\n\n\n

The CX-A is equipped with an LED and 3 filter sets to optionally perform widefield fluorescence microscopy in the Dapi, FitC and TritC\/Cy5 channels.<\/p>\n\n\n\n

The EVE Analytics software additionally provides an integrated image analysis and quantification tool that delivers analysis of cell morphology, composition and population dynamics.<\/p>\n\n\n\n

For more information, check out the CX-A website<\/a>.<\/p>\n\n\n\n

\n\nAvailable Objective Lenses<\/summary>\n
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Brand<\/strong><\/th>Type<\/strong><\/th>Mag.
(Range)<\/strong><\/th>		NA<\/strong><\/th>Immersion<\/strong><\/th>F.o.V. [\u03bcm]<\/strong><\/th>Usage<\/strong><\/th><\/tr><\/thead>
 <\/td><\/td>60x<\/td>0.8<\/td>Air<\/td>90 x 90<\/td>Detection<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n
<\/div>\n\n\n\n
\n\nAvailable illuminations<\/summary>\n
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Imaging type<\/strong><\/th>Wavelength [nm]<\/strong><\/th><\/tr><\/thead>
Holotomography interference<\/td>520<\/td><\/tr>
Epifluorescence<\/td>LED<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n
<\/div>\n\n\n\n
\n\nAvailable filter sets<\/summary>\n
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Dapi + FitC + TritC\/Cy5<\/p>\n\n<\/div>\n<\/details>\n\n\n\n

<\/div>\n\n\n\n
\n\nResolution<\/summary>\n
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Holotomography: x,y,z: 200 x 200 x 400 nm<\/p>\n\n\n\n

Epifluorescence: x,y: ~ 400 nm<\/p>\n\n<\/div>\n<\/details>\n\n\n\n

<\/p>\n\n<\/div>\n<\/div>\n\n\n

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