{"id":474,"date":"2025-07-22T10:12:02","date_gmt":"2025-07-22T10:12:02","guid":{"rendered":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/?page_id=474"},"modified":"2025-07-23T09:53:00","modified_gmt":"2025-07-23T09:53:00","slug":"lcs-spim","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/equipment\/lcs-spim\/","title":{"rendered":"Luxendo LCS SPIM"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The LCS SPIM is a compact light-sheet microscope designed to scan large optically cleared samples. Samples can be fit in three cuvette sizes -from [20 mm \u00d7 20 mm \u00d7 20 mm] up to [100 mm x 30 mm x 30 mm] (l \u00d7 w \u00d7 h)- allowing imaging from organs to organisms.<\/p>\n\n\n\n<p>LCS SPIM is an inverted setup with dual-side illumination and an extended field of view (FOV). It has the option of de-striping and also a TAG lens to get a uniform light-sheet across the FOV.<\/p>\n\n\n\n<p>The LuxBundle processing software allows stitching and processing data from large cleared samples.<\/p>\n\n\n\n<p>For more information, check out the&nbsp;<a href=\"https:\/\/www.bruker.com\/en\/products-and-solutions\/fluorescence-microscopy\/light-sheet-microscopes\/lcs-spim.html\">LCS SPIM website<\/a>.<\/p>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDetection objective<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-stripes\"><table><thead><tr><th><\/th><th><strong>Eff. Magnification<\/strong><\/th><th><strong>FOV (\u00b5m)<\/strong><\/th><th><strong>Pixel size (nm)<\/strong><\/th><th><strong>Optical resolution<\/strong><\/th><\/tr><\/thead><tbody><tr><td><\/td><td>2.2x<br><\/td><td>5990<\/td><td>2925<\/td><td><\/td><\/tr><tr><td><strong>Olympus 4x 0.28 NA<\/strong> <strong>air<\/strong><\/td><td>4.4x<\/td><td>2995<\/td><td>1463<\/td><td>1.1\u00b5m<\/td><\/tr><tr><td><\/td><td>6.7x<\/td><td>1997<\/td><td>975<\/td><td><\/td><\/tr><tr><td><\/td><td>8.9x<\/td><td>1497<\/td><td>731<\/td><td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nAvailable lasers<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th><strong>Wavelength [nm]<\/strong><\/th><\/tr><\/thead><tbody><tr><td>405<\/td><\/tr><tr><td>488<\/td><\/tr><tr><td>515<\/td><\/tr><tr><td>561<\/td><\/tr><tr><td>642<\/td><\/tr><tr><td>685<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nAvailable filters<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table\"><table><thead><tr><th><strong>Filters<\/strong><\/th><\/tr><\/thead><tbody><tr><td>BP 418-462<\/td><\/tr><tr><td>LP 498<\/td><\/tr><tr><td>BP 500-530<\/td><\/tr><tr><td>LP 530<\/td><\/tr><tr><td>BP 526-564<\/td><\/tr><tr><td>LP 572<\/td><\/tr><tr><td>BP 580-627<\/td><\/tr><tr><td>LP 656<\/td><\/tr><tr><td>BP 655-704<\/td><\/tr><tr><td>LP 696<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-video\"><video style=\"max-width: 100%;\" controls src=\"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-content\/uploads\/2025\/07\/mouseEmbryo_substack_jpg.mp4\"><\/video><figcaption class=\"vf-figure__caption\">Cleared mouse embryo labeled with methylene blue (cyan) and showing autofluorescence (magenta) scanned with the LCS SPIM. Sample processed by Montserrat Coll Llad\u00f3, EMBL Barcelona.<\/figcaption><\/figure>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<style>\n.wp-block-table {\n  width: 100%;\n  overflow-x: auto;\n}\n\n.wp-block-table table {\n  width: 100%;\n  table-layout: auto;\n  border-collapse: collapse;\n}\n\n.wp-block-table th,\n.wp-block-table td {\n  word-wrap: break-word;\n  white-space: normal;\n}\n<\/style>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":322,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-474","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/pages\/474","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/comments?post=474"}],"version-history":[{"count":6,"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/pages\/474\/revisions"}],"predecessor-version":[{"id":655,"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/pages\/474\/revisions\/655"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/pages\/322"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/media?parent=474"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/wp-json\/wp\/v2\/embl_taxonomy?post=474"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}