{"id":112,"date":"2021-05-19T15:49:36","date_gmt":"2021-05-19T15:49:36","guid":{"rendered":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/?page_id=112"},"modified":"2025-07-17T12:35:23","modified_gmt":"2025-07-17T12:35:23","slug":"opt1-custom","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/equipment\/opt1-custom\/","title":{"rendered":"OPT1 – Custom"},"content":{"rendered":"\n
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\n\nAvailable objective lenses<\/summary>\n
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Brand<\/strong><\/th>Type<\/strong><\/th> Mag.<\/strong><\/th>Zoom range<\/strong><\/th>NA<\/strong><\/th> Immersion (ref. ind.)<\/strong><\/th> WD [mm]<\/strong><\/th> F.o.V. [mm]<\/strong><\/th><\/tr><\/thead>
 Leica<\/td>Plan- achromatic<\/td>0.5x Obj, 0.63x C-mount<\/td>0.226x to 3.62x<\/td> 0.14 (nominal)<\/td>Air (1.0)<\/td>135<\/td>2.393 x 1.823 to 38.32 x 29.19<\/td><\/tr><\/tbody><\/table>
F.o.V. = field of view, with the Hamamatsu Orca ER camera (1344 x 1024 pixels, 6.45 \u03bcm pitch)<\/figcaption><\/figure>\n\n\n\n

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\n\nAvailable filter sets<\/summary>\n
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Type<\/strong><\/th>Exciter filter<\/strong><\/th>Barrier filter<\/strong><\/th><\/tr><\/thead>
GFP1<\/td>395-455 nm<\/td>480 LP<\/td><\/tr>
GFP2<\/td>460-500 nm<\/td>510 LP<\/td><\/tr>
 GFP3<\/td>450-490 nm<\/td>500-550 nm<\/td><\/tr>
UV<\/td>340-380 nm<\/td>420 LP<\/td><\/tr>
CFP<\/td>426-446 nm<\/td>460-500 nm<\/td><\/tr>
YFP<\/td>490-510 nm<\/td>520-550 nm<\/td><\/tr>
Texas Red<\/td>590-650 nm<\/td>610 LP<\/td><\/tr>
DsRED<\/td>530-560 nm<\/td>590-650 nm<\/td><\/tr>
Cy5<\/td>590-650 nm<\/td>573-648 nm<\/td><\/tr><\/tbody><\/table>
Fluorescence excitation light source:<\/strong>
High-intensity mercury lamp 100 W, lamp housing 106 z, chromatically corrected collector, focusable, centrable lamp mount<\/figcaption><\/figure>\n\n\n\n

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