{"id":110,"date":"2021-05-19T15:49:09","date_gmt":"2021-05-19T15:49:09","guid":{"rendered":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/?page_id=110"},"modified":"2025-07-17T12:10:03","modified_gmt":"2025-07-17T12:10:03","slug":"optispim1","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/mesoscopic-imaging-facility\/equipment\/optispim1\/","title":{"rendered":"OPTiSPIM1"},"content":{"rendered":"\n
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\n\nAvailable objective lenses<\/summary>\n
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Brand<\/strong><\/th>Type<\/strong><\/th>Mag.<\/strong><\/th>NA<\/strong><\/th>Immersion<\/strong><\/th> WD [mm]<\/strong><\/th>F.o.V. [\u03bcm]<\/strong><\/th>Illum. lens<\/strong><\/th><\/tr><\/thead>
 Leica<\/td>N PLAN<\/td>2.5<\/td>0.07<\/td>Air<\/td>11.2<\/td>2642 x 3468<\/td>–<\/td><\/tr>
 Leica<\/td>PLAN<\/td>4<\/td>0.1<\/td>Air<\/td>26.2<\/td> 1651 x 2167<\/td>–<\/td><\/tr>
 Leica<\/td>N PLAN EPI<\/td>5<\/td> 0.12<\/td>Air<\/td>14.0<\/td> 1321 x 1734<\/td>#1<\/td><\/tr>
 Leica<\/td>HC PL FLUOTAR<\/td>10<\/td>0.3<\/td>Air<\/td>11.2<\/td> 660 x 867<\/td>#3<\/td><\/tr>
 Leica<\/td>N PLAN L<\/td>20<\/td>0.4<\/td>Air<\/td>10.8<\/td> 330 x 433<\/td>#3<\/td><\/tr>
 Leica<\/td>HCX APO L<\/td>10<\/td>0.3<\/td> Water<\/td>3.6<\/td> 660 x 867<\/td>#3<\/td><\/tr>
 Leica<\/td>HCX APO L<\/td>20<\/td>0.5<\/td> Water<\/td>3.5<\/td>  330 x 433<\/td>#3<\/td><\/tr>
 Leica<\/td>HCX APO L<\/td>40<\/td>0.8<\/td> Water<\/td>3.3<\/td> 165 x 217<\/td>#4<\/td><\/tr>
 Leica<\/td>HCX APO L<\/td>63<\/td>0.9<\/td> Water<\/td>2.2<\/td> 105 x 138<\/td>#4<\/td><\/tr><\/tbody><\/table>
F.o.V. = field of view size, with the Hamamatsu Orca ER camera (1344×1024 pixels, 6.45\u03bcm pitch)
Illum. lens = “optimal” lens for SPIM illumination<\/figcaption><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n

<\/p>\n\n\n\n

\n\nAvailable lasers<\/summary>\n
\n\n
Type<\/strong><\/th>Wavelength [nm]<\/strong><\/th>Power [mW]<\/strong><\/th><\/tr><\/thead>
 Diode<\/td>405<\/td>100<\/td><\/tr>
Diode<\/td>488<\/td>50<\/td><\/tr>
Diode <\/td>515<\/td>80<\/td><\/tr>
HeNe<\/td>543<\/td>1<\/td><\/tr>
OPSL <\/td>594<\/td>100<\/td><\/tr>
 Diode<\/td>638<\/td>50<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n

<\/p>\n\n\n\n

\n\nAvailable filters, SPIM filter wheel<\/summary>\n
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Type<\/strong><\/th> Fluorphore(s)<\/strong><\/th>Position<\/strong><\/th><\/tr><\/thead>
Opaque<\/td> <\/td>0<\/td><\/tr>
Clear<\/td> <\/td>1<\/td><\/tr>
525 BP 50<\/td>GFP, Alexa 488<\/td>2<\/td><\/tr>
585 BP 60<\/td>dsRed, Alexa 543, Cy3<\/td>3<\/td><\/tr>
700 BP 75<\/td>draq5, Alexa 633<\/td>4<\/td><\/tr>
594 notch 22<\/td> <\/td> –<\/td><\/tr>
490 BP 40<\/td>CFP<\/td> –<\/td><\/tr>
RS 514 LP<\/td> <\/td> –<\/td><\/tr>
RS 488 LP<\/td> <\/td>8<\/td><\/tr>
RS 458 LP<\/td> <\/td> –<\/td><\/tr>
RS 633 LP<\/td> <\/td>6<\/td><\/tr>
 624 BP 40<\/td> Alexa 594<\/td>7<\/td><\/tr>
 447 BP 60<\/td> Alexa 405<\/td>9<\/td><\/tr>
 dualband 512\/630<\/td> GFP & DsRed<\/td> –<\/td><\/tr>
 550 BP 49<\/td> YFP<\/td>5<\/td><\/tr><\/tbody><\/table>
Position = current position in filter wheel<\/figcaption><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n

<\/p>\n\n\n\n

\n\nAvailable filter sets, OPT filter slider<\/summary>\n
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<\/p>\n\n\n\n

Type<\/strong><\/th>Position<\/strong><\/th><\/tr><\/thead>
DAPI<\/td>1<\/td><\/tr>
CFP<\/td> –<\/td><\/tr>
eGFP<\/td>4<\/td><\/tr>
DsRed<\/td>2<\/td><\/tr>
TXR<\/td>5<\/td><\/tr>
 FITC (lp)<\/td> –<\/td><\/tr>
 YFP<\/td> –<\/td><\/tr><\/tbody><\/table>
Position = current position in filter wheel<\/figcaption><\/figure>\n\n\n\n

<\/p>\n\n<\/div>\n<\/details>\n\n\n\n

<\/div>\n\n\n\n

<\/p>\n\n\n\n