{"id":3437,"date":"2024-10-01T08:41:58","date_gmt":"2024-10-01T08:41:58","guid":{"rendered":"https:\/\/www.embl.org\/groups\/light-imaging-facility\/?page_id=3437"},"modified":"2025-04-10T08:28:32","modified_gmt":"2025-04-10T08:28:32","slug":"equipment","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/light-imaging-facility\/equipment\/","title":{"rendered":"Equipment"},"content":{"rendered":"\n

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Microscopes<\/h2>\n\n\n\n
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Microscope<\/th>Main features<\/th>Objectives<\/th><\/tr><\/thead>
Nikon AX\/AR<\/td>Laser Scanning confocal microscope. 4 detectors, 2 APD’s and 2 PMT’s. 4 lasers available (405, 488, 565, 640). Driven by NIS-Elements<\/td>4x, 10x, 20x NA 0.75, 40x dry NA 0.95, 60x NA 1.42 oil immersion, 25x NA 1.05 silicon oil and 40x NA 1.3 silicon oil. Last two objectives upon request.<\/td><\/tr>
Leica Thunder<\/td>Fast epifluorescence microscope. LED engine with 405, 475, 555, 635 and 730. sCMOS camera Zyla. “Computational clearing” (deblurring) as post-processing available.<\/td>5x, 10x, 20x LWD NA 0.4 (for plastic), 20x NA 0.8, 63x oil<\/td><\/tr>
Crest Optics V3 and DeepSIM<\/td>System has confocal, epifluorescence and super-resolution modes. 7 lasers available (405, 446, 488, 518, 545, 637 and 748 nm). sCMOS camera Prime 95B. Driven by NIS-Elements<\/td>4x, 10x, 20x NA 0.75, 40x dry NA 0.95, 60x NA 1.42 oil immersion, 25x NA 1.05 silicon oil and 40x NA 1.3 silicon oil. Last two objectives upon request.<\/td><\/tr>
Olympus VS200
Automated slide scanner. <\/td>		Xylis LED light engine. Orca Flash V2 sCMOS camera. Automated oil dispenser. Slide tray loader for more than 200 slides.<\/td>2x, 4x, 10x, 20x NA 0.8, 40x dry NA 0.95 and 40x oil NA 1.3.<\/td><\/tr>
Alpenglow OTLS<\/td>Hybrid open-top light sheet microscope. 4 lasers available, 405, 488, 656 and 640. 2 PCO sCMOS cameras.<\/td>2 detection paths, an ODO equivalent to a 5x and a NODO equivalent to a 20x.<\/td><\/tr>
SMZ25<\/td>Motorized stereo microscope with fluorescence. Filters available for GFP and mCherry. Qi CCD camera. NIS-Elements<\/td>1x and 2x<\/td><\/tr>
Abberior STEDYCON<\/td>Compact STED system. Available lasers for confocal 405, 488, 565 and 640. Available STED laser<\/td>100x<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
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Image analysis<\/h2>\n\n\n\n
Resource<\/th>Main Features<\/th><\/th><\/tr><\/thead>
Workstation<\/td>GPU<\/td><\/td><\/tr>
Imaris<\/td><\/td><\/td><\/tr>
AIVIA<\/td><\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

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Tissue processing<\/h2>\n\n\n\n
Instrument<\/th>Room<\/th><\/tr><\/thead>
Leica Cryostat CM3050S<\/td>59<\/td><\/tr>
Cryostat Thermo Scientific NX70<\/td>59<\/td><\/tr>
Microtome Leica RM2135 – manual<\/td>59<\/td><\/tr>
Microtome Leica RM2135 – semi-automated<\/td>59<\/td><\/tr>
Vibratome Leica VT1000S (2 units)<\/td>59<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

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