The Gross Group is pleased to provide the scientific community with the following recombinant adeno-associated viral (AAV) vectors for neuroscience applications.<\/p>\n\n\n\n
<\/p>\n\n\n\n
These vectors are produced to contain the capsid proteins of both AAV1 and AAV2 for efficient heparin-based purification and broad tropism. For additional information regarding the production and purification procedure, refer to:<\/p>\n\n\n\n
Hauck B, Chen L, Xiao W.<\/strong> Generation and characterization of chimeric recombinant AAV vectors. Mol Ther.<\/em> 2003 Mar;7(3)<\/em> 419-425. doi:10.1016\/s1525-0016(03)00012-1<\/a>. PMID: 12668138<\/a>; PMCID: PMC2636682<\/a>.<\/p>\n\n\n\n McClure C, Cole KL, Wulff P, Klugmann M, Murray AJ.<\/strong> Production and titering of recombinant adeno-associated viral vectors. J Vis Exp.<\/em> 2011 Nov e3348. doi:10.3791\/3348<\/a>. PMID: 22143312<\/a>; PMCID: PMC3308604<\/a>.<\/p>\n\n\n\n Both DNA constructs and AAVs are available upon request. Please address requests for materials and information to Cornelius Gross (gross@embl.it<\/a>) and Angelo Raggioli (raggioli@embl.it<\/a>).<\/p>\n\n\n\n eSyn::iRFP670 Ef1a::Synaptophysin-GFP CMV::FLEX-hM4D-invVenus hSyn::SNAP hSyn::Venus-2A-CreERT2<\/p>\n\n\n\n hSyn::tdTomato; hU6::GFP-targeting sgRNA These vectors are produced to contain the capsid proteins of both AAV1 and AAV2 for efficient heparin-based purification and broad tropism. For additional information regarding the production and purification procedure, refer to: Hauck B, Chen L, Xiao W. Generation and characterization of…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-with-sidebar.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-71","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/pages\/71","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/comments?post=71"}],"version-history":[{"count":4,"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/pages\/71\/revisions"}],"predecessor-version":[{"id":262,"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/pages\/71\/revisions\/262"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/media?parent=71"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gross\/wp-json\/wp\/v2\/embl_taxonomy?post=71"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Available AAVs<\/h3>\n\n\n\n
Cytoplasmic and membrane labeling AAVs<\/h4>\n\n\n\n
rSyn::iRFP670
rSyn::iRFP670-farnesyl
hSyn::tdTomato
hSyn::tdTomato-farnesyl<\/p>\n\n\n\nPre-synaptic labeling AAVs<\/h4>\n\n\n\n
Ef1a::Synaptophysin-iRFP<\/p>\n\n\n\nActivity modulating AAVs<\/h4>\n\n\n\n
hSyn::Venus-P2A-hM4D<\/p>\n\n\n\nSNAP\/Halo expressing AAVs<\/h4>\n\n\n\n
hSyn::GPI-SNAP
hSyn::GPI-Halo<\/p>\n\n\n\nRecombinase expressing AAVs<\/h4>\n\n\n\n
CRISPR AAVs<\/h4>\n\n\n\n
hSyn::tdTomato; hU6::empty<\/p>\n","protected":false},"excerpt":{"rendered":"