{"id":720,"date":"2023-05-10T12:27:36","date_gmt":"2023-05-10T12:27:36","guid":{"rendered":"https:\/\/www.embl.org\/groups\/gentechdev\/?page_id=720"},"modified":"2025-12-04T10:26:57","modified_gmt":"2025-12-04T10:26:57","slug":"instrumentation","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/gentechdev\/instrumentation\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-microscopy\" data-vf-js-location-nearest-activation-target=\"\">Microscopy<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-micro-fluidics\" data-vf-js-location-nearest-activation-target=\"\">Microfluidics<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-image-analysis\" data-vf-js-location-nearest-activation-target=\"\">Image Analysis<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-microscopy\"><h2>Microscopy<\/h2>\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"225\" src=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3137-1024x768-1-300x225.jpg\" class=\"vf-summary__image\" alt=\"confocal spinning disk\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3137-1024x768-1-300x225.jpg 300w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3137-1024x768-1-768x576.jpg 768w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3137-1024x768-1.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    Nikon Yokogawa spinning disk confocal\n    <\/h3>\n\n  <p style=\"font-weight: 400;\">To perform automated sequential imaging, we use a Nikon microscope coupled to a Yokogawa CSU-W1 confocal scanner. It includes a 405 nm, 488 nm, 561 nm, 638 nm and a 690 nm laser for barcode demultiplexing in four channels.<\/p>\n<\/article>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-micro-fluidics\"><h2>Microfluidics<\/h2>\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"296\" src=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_0351-1024x1012-1-300x296.jpg\" class=\"vf-summary__image\" alt=\"Elveflow sequential injection\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_0351-1024x1012-1-300x296.jpg 300w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_0351-1024x1012-1-768x759.jpg 768w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_0351-1024x1012-1.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    Elveflow sequential injection system\n    <\/h3>\n\n  <p style=\"font-weight: 400;\">This custom-built microfluidics system comprises several distribution valves and enables the automated delivery of up to 47 solutions into a microfluidics imaging chamber.<\/p>\n<\/article>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"225\" src=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3133-1024x768-1-300x225.jpg\" class=\"vf-summary__image\" alt=\"Aria Fluigent sequential injection\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3133-1024x768-1-300x225.jpg 300w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3133-1024x768-1-768x576.jpg 768w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/IMG_3133-1024x768-1.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    Aria Fluigent sequential injection system\n    <\/h3>\n\n  <p style=\"font-weight: 400;\">This system can be used for the automated delivery of up to 10 different solutions into a microfluidic imaging chamber. For automated sequential imaging experiments, the device can be synchronised with a microscope using a TTL signal.<\/p>\n<\/article>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-image-analysis\"><h2>Image Analysis<\/h2>\n<p><\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"703\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/3-Untitled-slide-e1684932623844-1024x703.png\" alt=\"\" class=\"wp-image-1234\" style=\"width:-191px;height:-131px\" srcset=\"https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/3-Untitled-slide-e1684932623844-1024x703.png 1024w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/3-Untitled-slide-e1684932623844-300x206.png 300w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/3-Untitled-slide-e1684932623844-768x527.png 768w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/3-Untitled-slide-e1684932623844-1536x1054.png 1536w, https:\/\/www.embl.org\/groups\/gentechdev\/wp-content\/uploads\/2023\/05\/3-Untitled-slide-e1684932623844-2048x1405.png 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Schematic of data analysis workflow<\/figcaption><\/figure>\n\n\n\n<p>For processing and analysing imaging datasets at scale, we can offer remote access to high powered virtual machines via the <a rel=\"noreferrer noopener\" href=\"https:\/\/www.embl.org\/about\/info\/it-services\/3d-cloud\/\" target=\"_blank\">EMBL 3D cloud<\/a> infrastructure.<\/p>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-card vf-card--brand vf-card--striped vf-u-margin__bottom--800\" default>\n  <div class=\"vf-card__content | vf-stack vf-stack--400\">\n      <h3 class=\"vf-card__heading\">\n              <a class=\"vf-card__link\" href=\"https:\/\/www.embl.org\/groups\/gentechdev\/?page_id=662\">\n      Technologies       <svg aria-hidden=\"true\" class=\"vf-card__heading__icon | vf-icon vf-icon-arrow--inline-end\" width=\"1em\" height=\"1em\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n          <path d=\"M0 12c0 6.627 5.373 12 12 12s12-5.373 12-12S18.627 0 12 0C5.376.008.008 5.376 0 12zm13.707-5.209l4.5 4.5a1 1 0 010 1.414l-4.5 4.5a1 1 0 01-1.414-1.414l2.366-2.367a.25.25 0 00-.177-.424H6a1 1 0 010-2h8.482a.25.25 0 00.177-.427l-2.366-2.368a1 1 0 011.414-1.414z\" fill=\"currentColor\" fill-rule=\"nonzero\"><\/path>\n       <\/svg>\n        <\/a>\n          <\/h3>\n            <p class=\"vf-card__subheading\">Available techniques at GenTechDev<\/p>\n          <\/div>\n<\/article>\n\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-720","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/pages\/720","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/comments?post=720"}],"version-history":[{"count":16,"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/pages\/720\/revisions"}],"predecessor-version":[{"id":23439,"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/pages\/720\/revisions\/23439"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/media?parent=720"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gentechdev\/wp-json\/wp\/v2\/embl_taxonomy?post=720"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}