{"id":40,"date":"2024-01-12T10:11:37","date_gmt":"2024-01-12T10:11:37","guid":{"rendered":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/?page_id=40"},"modified":"2024-02-01T09:21:43","modified_gmt":"2024-02-01T09:21:43","slug":"services","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/services\/","title":{"rendered":"Services"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>Our unit operates on a complete-service fully costed model, tailoring our charges based on researcher affiliation.<\/p>\n\n\n\n<p>For advice on using CRISPR\/Cas9 in your research or should you require a novel transgenic mouse or cell line, please email: <a href=\"mailto:gevf@embl.it\">gevf@embl.it<\/a><\/p>\n\n\n\n<p>For advice on using viruses for your research or ordering a virus, please email: <a href=\"mailto:viruses@embl.it\">viruses@embl.it<\/a><\/p>\n\n\n\n<p><strong>Mouse and cell service elements<\/strong> <strong>include:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>CRISPR\/Cas9 project design and reagent sourcing<\/li>\n\n\n\n<li>Zygote pronuclear microinjection<\/li>\n\n\n\n<li>Surgical embryo implantation<\/li>\n\n\n\n<li>Microinjection of targeted ES cells into host 8-cell embryos<\/li>\n\n\n\n<li>Sequence confirmation of F1 genotype<\/li>\n\n\n\n<li>Cryopreservation of embryos and\/or sperm<br>IVF rederivation<\/li>\n\n\n\n<li>Import and rederivation of mouse lines into the LAR facility<\/li>\n<\/ul>\n\n\n\n<p><strong>Virus service elements include:<\/strong><\/p>\n\n\n\n<p>Production of custom-made purified and quantitated viral stocks that are ready for cargo delivery to the desired cell type.<\/p>\n\n\n\n<p><em>Recombinant Adeno-Associated Virus (rAAV)<\/em><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Vector capacity: 4.7Kb<\/li>\n\n\n\n<li>Cargo remains episomal<\/li>\n\n\n\n<li>Current serotypes in stock include rAAV1, 2, 1\/2 chimeric, 2-retro, DJ, DJ\/8, DJ\/9, 5, 6, 7, 8, 8BP2, 9, PHP.S, PHP.EB, B10, and 7m8.&nbsp;<\/li>\n\n\n\n<li>Please let us know if you require another serotype and we will do what we can to obtain the necessary reagents.<\/li>\n\n\n\n<li>Typically provide at least 200\u03bcl of \u22651\u00d71012 VG\/ml but smaller or larger quantities can be provided at request.<\/li>\n<\/ul>\n\n\n\n<p><em>Lentivirus<\/em><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Vector capacity: 8Kb<\/li>\n\n\n\n<li>Cargo integrates<\/li>\n\n\n\n<li>Currently, we utilise VSV-G due to its wide range of infectivity. Other envelope proteins could be offered if requested. Currently, viruses are produced using either the 2nd or 3rd generation.<\/li>\n<\/ul>\n\n\n\n<p><em>Herpes Simplex Virus (HSV)<\/em><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Vector capacity: 30Kb<\/li>\n\n\n\n<li>Cargo remains episomal<\/li>\n<\/ul>\n\n\n\n<p><em>DNA constructs<\/em><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Long ssDNA (up to ~5kb) for CRISPR\/Cas9-mediated knock-in<\/li>\n\n\n\n<li>Modification of DNA, precise to the base pair<\/li>\n\n\n\n<li>Targeting construct to make a knock-in or knockout<\/li>\n\n\n\n<li>General sub-cloning service<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-40","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/pages\/40","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/comments?post=40"}],"version-history":[{"count":6,"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/pages\/40\/revisions"}],"predecessor-version":[{"id":277,"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/pages\/40\/revisions\/277"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/media?parent=40"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/gene-editing-and-virus-facility\/wp-json\/wp\/v2\/embl_taxonomy?post=40"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}