{"id":621,"date":"2021-02-07T17:04:23","date_gmt":"2021-02-07T17:04:23","guid":{"rendered":"https:\/\/www.embl.org\/groups\/galej\/?p=621"},"modified":"2021-08-27T15:48:06","modified_gmt":"2021-08-27T15:48:06","slug":"new-paper-on-the-integrator-complex","status":"publish","type":"post","link":"https:\/\/www.embl.org\/groups\/galej\/blog\/2021\/02\/new-paper-on-the-integrator-complex\/","title":{"rendered":"A new paper on the Integrator complex"},"content":{"rendered":"\n<p>Our newest paper describing the <strong>structure of the catalytic core of the Integrator complex<\/strong> is online now in <a href=\"https:\/\/authors.elsevier.com\/sd\/article\/S1097-2765(21)00005-8\">Molecular Cell<\/a>. <\/p>\n\n\n\n<p>Integrator complex was discovered more than 15 years ago as a specialised endonuclease responsible for the <strong>3\u2019-end processing<\/strong> of spliceosomal snRNAs. More recent studies provided compelling evidence that it is involved in genome-wide transcription attenuation of thousands of transcripts from different classes.<\/p>\n\n\n\n<p>Using cell biology and proteomics, <strong>Moritz Pfleiderer<\/strong> identified stable modules within this complex, expressed them recombinantly and determined the structure of one of them by <strong>cryo-EM.<\/strong><\/p>\n\n\n\n<p>The structure provides new insights into the arrangement and assembly mechanism of the catalytic nuclease heterodimer, INTS9\/11, and the recruitment of a helical protein INTS4. All three proteins form a charged cavity, which is likely involved in guiding RNA towards the endonuclease active site.<br>Comparison with the related CPSF complex shows some interesting differences in the spatial organisation of the two complexes.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.embl.org\/news\/science\/at-the-core-of-the-integrator-complex\/\">EMBL News<\/a><\/p>\n\n\n\n<figure class=\"vf-figure wp-block-video\"><video style=\"max-width: 100%;\" height=\"1068\" style=\"aspect-ratio: 1314 \/ 1068;\" width=\"1314\" autoplay loop src=\"https:\/\/www.embl.org\/groups\/galej\/wp-content\/uploads\/2021\/03\/movie-1.mp4\"><\/video><\/figure>\n\n\n\n<figure class=\"wp-block-embed is-type-rich is-provider-twitter wp-block-embed-twitter\"><div class=\"vf-embed vf-embed--16x9\">\n<blockquote class=\"twitter-tweet\" data-width=\"500\" data-dnt=\"true\"><p lang=\"en\" dir=\"ltr\">Very happy to share our new paper describing the structure of the catalytic core of the Integrator complex, published today in <a href=\"https:\/\/twitter.com\/MolecularCell?ref_src=twsrc%5Etfw\">@MolecularCell<\/a> . Fantastic work of <a href=\"https:\/\/twitter.com\/MorPfl?ref_src=twsrc%5Etfw\">@MorPfl<\/a> the first PhD student in my group <a href=\"https:\/\/twitter.com\/embl?ref_src=twsrc%5Etfw\">@embl<\/a> Grenoble!<a href=\"https:\/\/t.co\/NnCSu4IOuw\">https:\/\/t.co\/NnCSu4IOuw<\/a> <a href=\"https:\/\/t.co\/jmJwZlqWaz\">pic.twitter.com\/jmJwZlqWaz<\/a><\/p>&mdash; Wojtek Galej (@WojtekGalej) <a href=\"https:\/\/twitter.com\/WojtekGalej\/status\/1357725523049406468?ref_src=twsrc%5Etfw\">February 5, 2021<\/a><\/blockquote><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script>\n<\/div><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Our newest paper describing the structure of the catalytic core of the Integrator complex is online now in Molecular Cell. Integrator complex was discovered more than 15 years ago as a specialised endonuclease responsible for the 3\u2019-end processing of spliceosomal snRNAs. More recent studies&hellip;<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2029],"tags":[],"embl_taxonomy":[],"class_list":["post-621","post","type-post","status-publish","format-standard","hentry","category-science"],"acf":[],"embl_taxonomy_terms":[],"featured_image_src":"https:\/\/www.embl.org\/groups\/galej\/wp-includes\/images\/media\/default.svg","_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/posts\/621","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/comments?post=621"}],"version-history":[{"count":8,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/posts\/621\/revisions"}],"predecessor-version":[{"id":970,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/posts\/621\/revisions\/970"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/media?parent=621"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/categories?post=621"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/tags?post=621"},{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/galej\/wp-json\/wp\/v2\/embl_taxonomy?post=621"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}