{"id":14,"date":"2020-05-22T08:59:49","date_gmt":"2020-05-22T08:59:49","guid":{"rendered":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/home\/"},"modified":"2025-01-15T13:57:20","modified_gmt":"2025-01-15T12:57:20","slug":"home","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/","title":{"rendered":"Home"},"content":{"rendered":"<div class=\"vf-grid vf-grid__col-3 | vf-u-margin__bottom--800\">\n      <div class=\"vf-grid__col--span-2\">\n      <div class=\"vf-content-hub-html\">\n  <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=profiles&amp;filter-uuid=7fc947bf-6dee-44d4-8280-e30a14ab129e&amp;pattern=node-teaser -->\n      <div data-embl-js-conditional-edit=\"27814\">\n              <h1 class=\"vf-lede\">The Flow Cytometry Facility (FCF) provides instrumentation, education, and expertise for all flow cytometry needs.<\/p>\r\n\n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/27814\" target=\"_blank\">Edit<\/a>\n    <\/div>\n  <\/div>\n    <\/div>\n      <div >\n\n<!-- <style>\n  .vf-content-hub-html {\n    --vf-stack-margin--custom: unset !important;\n  }\n<\/style> -->\n\n    <div class=\"vf-content-hub-html vf-stack vf-stack--600\" data-cache=\"ca9b5390\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-value%5Bfield_person_positions.entity.field_position_membership%5D=leader&amp;filter-field-value%5Bfield_person_positions.entity.field_position_team.entity.field_foreignid%5D=463&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bteam%2Cmobile%2Cphones%5D=1&amp;limit=5&amp;pattern=vf-profile-inline&amp;sort-field-value%5Bchanged%5D=DESC -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"133334\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60036603.jpg?itok=5PhJkkeo\" alt=\"image of Gerald Pfister\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/gerald-pfister\" class=\"vf-profile__link\">Gerald Pfister<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-profile__job-title\">\n          Head of Flow Cytometry Facility\n        <\/p>\n      \n      \n      \n      \n      \n      \n              <p class=\"vf-profile__uuid\">\n          <span>ORCID:<\/span>\n          <a class=\"vf-profile__link vf-profile__link--secondary\" href=\"https:\/\/europepmc.org\/authors\/0000-0003-4928-6612\">\n            0000-0003-4928-6612\n          <\/a>\n        <\/p>\n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/133334\/133334\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n\n  <\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n  About  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The FCF provides instrumentation, education, and expertise for all flow cytometry needs. We encourage you to explore our services and contact us for any inquiries.<\/p>\n\n\n\n<p>Besides common wet lab equipment, the FCF houses an Attune NxT benchtop analyzer (4 lasers), a BD FACSARIA Fusion cell sorter in a BSL-2 hood (5 lasers), a BD FACSMelody cell sorter (3 lasers), and a Chromium Controller from 10X Genomics for single cell profiling.<\/p>\n\n\n\n<p>Analyzers and sorters are available for self-service use after operator training has been successfully completed. Assisted services are offered for non-trained users upon request. All services can be booked directly by registered users via our online booking system <a href=\"https:\/\/ppms.embl.de\/?RomeFCF\" target=\"_blank\" rel=\"noreferrer noopener\">here<\/a>.<\/p>\n\n\n\n<p>A wide range of applications including gene reporter assays, single cell cloning, gene expression analysis, multicolor cell immunophenotyping, cell cycle analysis, intracellular signaling analysis, cytokine production, etc, may be performed.<\/p>\n\n\n\n<p>The FCF is available for use by external groups. Please contact us for details.<\/p>\n\n\n\n<p class=\"has-text-align-center\"><\/p>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1357\" height=\"81\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/Screenshot-2025-01-15-135422.jpg\" alt=\"\" class=\"wp-image-16261\" style=\"width:771px;height:auto\" srcset=\"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/Screenshot-2025-01-15-135422.jpg 1357w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/Screenshot-2025-01-15-135422-300x18.jpg 300w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/Screenshot-2025-01-15-135422-1024x61.jpg 1024w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/Screenshot-2025-01-15-135422-768x46.jpg 768w\" sizes=\"auto, (max-width: 1357px) 100vw, 1357px\" \/><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"vf-figure wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"3083\" height=\"1169\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a.png\" alt=\"\" class=\"wp-image-16237\" style=\"width:1450px;height:auto\" srcset=\"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a.png 3083w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a-300x114.png 300w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a-1024x388.png 1024w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a-768x291.png 768w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a-1536x582.png 1536w, https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-content\/uploads\/2025\/01\/FCF-Infrastructure-2025a-2048x777.png 2048w\" sizes=\"auto, (max-width: 3083px) 100vw, 3083px\" \/><\/figure>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n  Contact  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p><strong>Flow Cytometry Facility<\/strong><br><\/p>\n\n\n\n<p>EMBL Rome<br>Epigenetics and Neurobiology Unit<br>Via Ramarini 32<br>00015 Monterotondo (RM), Italy<br><br>Telephone: +39 06 900 91-340\/440<br>Email:&nbsp;<a href=\"mailto:facs@embl.it\">facs@embl.it<\/a><\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-14","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/pages\/14","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/comments?post=14"}],"version-history":[{"count":71,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/pages\/14\/revisions"}],"predecessor-version":[{"id":16265,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/pages\/14\/revisions\/16265"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/media?parent=14"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-rome\/wp-json\/wp\/v2\/embl_taxonomy?post=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}