{"id":14,"date":"2020-06-08T10:45:26","date_gmt":"2020-06-08T10:45:26","guid":{"rendered":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/home\/"},"modified":"2024-12-11T12:19:47","modified_gmt":"2024-12-11T12:19:47","slug":"home","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/","title":{"rendered":"Home"},"content":{"rendered":"<div class=\"vf-grid vf-grid__col-3 | vf-u-margin__bottom--800\">\n      <div class=\"vf-grid__col--span-2\">\n      <div class=\"vf-content-hub-html\">\n  <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=profiles&amp;filter-uuid=53113f1a-14eb-4788-b0e1-0f89a00845cb&amp;pattern=node-teaser -->\n      <div data-embl-js-conditional-edit=\"28178\">\n              <h1 class=\"vf-lede\">The Flow Cytometry Core Facility offers access to high-end FACS analysis and state-of-the-art cell sorting capabilities, actively providing scientists with adaptive setups and dedicated support.<\/p>\r\n\n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/28178\" target=\"_blank\">Edit<\/a>\n    <\/div>\n  <\/div>\n    <\/div>\n      <div >\n\n<!-- <style>\n  .vf-content-hub-html {\n    --vf-stack-margin--custom: unset !important;\n  }\n<\/style> -->\n\n    <div class=\"vf-content-hub-html vf-stack vf-stack--600\" data-cache=\"6514e9ef\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-value%5Bfield_person_positions.entity.field_position_membership%5D=leader&amp;filter-field-value%5Bfield_person_positions.entity.field_position_team.entity.field_foreignid%5D=251&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bteam%2Cmobile%2Cphones%5D=1&amp;limit=5&amp;pattern=vf-profile-inline&amp;sort-field-value%5Bchanged%5D=DESC -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"196233\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60100148.jpg?itok=O9aepGqd\" alt=\"image of Alex Cabrera Nu\u00f1ez\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/alex-claudio-cabrera-nunez\" class=\"vf-profile__link\">Alex Cabrera Nu\u00f1ez<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-profile__job-title\">\n          Head of Core Facility\n        <\/p>\n      \n      \n      \n      \n      \n      \n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/196233\/196233\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n\n  <\/div>\n<\/div>\n\n\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n  About  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>We offer a wide range of flow cytometric techniques, hands-on service, data analysis, and tailored training sessions for users inside and outside EMBL. Our facility strives to meet researchers&#8217; specific needs by providing dedicated help with experimental design, enabling the highest possible resolution in terms of analysis and product. We have recently upgraded the facility to provide access to S2 cell sorting services and improved our general sorting set up to meet the growing need for high-parametric and aseptic sorting.<\/p>\n\n\n\n<p>We work with equipment from Beckman Coulter, Becton Dickinson, Cytek Biosciences, and Coherent Inc. The EMBL Flow Cytometry platform is open to test new technological developments to serve the needs of the scientific community.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large is-style-default\"><a href=\"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"950\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image-1024x950.jpg\" alt=\"\" class=\"wp-image-235\" srcset=\"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image-1024x950.jpg 1024w, https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image-300x278.jpg 300w, https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image-768x712.jpg 768w, https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image-1536x1425.jpg 1536w, https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-content\/uploads\/2021\/10\/Home-NEW-Image.jpg 1833w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\"><strong>Figure 1:<\/strong><em>&nbsp;Flow cytomery applications used by EMBL researchers: Cell cycle analysis and Phenotyping of immune cells.<\/em><\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n  Major projects and accomplishments  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<ul class=\"wp-block-list\">\n<li>Full spectral cytometry for multi-parametric analysis of cell populations with high autofluorescence and\/or low levels of expression of key biomarkers.<\/li>\n\n\n\n<li>Single-cell sorting for single cell quantitative genome, transcriptome, CRISPR\/Cas9 genome editing and\u00a0<em>in vitro<\/em>\u00a0studies.<\/li>\n\n\n\n<li>Establishment of clonal cell lines carrying fluorescent protein-tagged genome-edited genes for 4D life cell imaging, and assessment of protein interactions and concentrations during mitosis.<\/li>\n\n\n\n<li>High-parametric S2 cell sorting service with dedicated equipment.<\/li>\n\n\n\n<li>High-throughput sorting of tissue-specific nuclei from\u00a0<em>Drosophila melanogaster\u00a0<\/em>embryo as a preparative step in the analysis of genome regulatory activity during tissue development.<\/li>\n\n\n\n<li>In-house Flow Cytometry course in cooperation with the German Cancer Research Center (DKFZ).<\/li>\n\n\n\n<li>Flow Cytometry data analysis workshops at EMBL-Heidelberg and EMBL-Rome.<\/li>\n\n\n\n<li>Five-day EMBL Course for Hands-on Flow Cytometry held once a year.\u00a0<\/li>\n\n\n\n<li>Five-day EMBO Practical Course on cell sorting held once every two years.\u00a0<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n  Services provided  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<ul class=\"wp-block-list\">\n<li>Complex multi-color analysis of cell populations based on light scatter and fluorescent probes content by conventional and spectral cytometry.<\/li>\n\n\n\n<li>Extensive support for basic and complex cell cycle analysis and apoptosis assays.<\/li>\n\n\n\n<li>Full-service sorting of rare populations out of a heterogeneous sample. Cell cloning, particle enrichment, and high purity bulk sorts.<\/li>\n\n\n\n<li>High-recovery and indexed single-cell sorting for sequencing.<\/li>\n\n\n\n<li>We offer to our researchers\u2019 advice in experimenrtal design and dedicated training sessions in the use of our flow cytometers, cell sorters and data analysis software<\/li>\n\n\n\n<li>We offer to our researchers&#8217; advice and dedicated training sessions in the use of flow cytometry, instrument operation, and post-acquisition data analysis.<\/li>\n\n\n\n<li>Developing of novel flow cytometric techniques to meet EMBL&#8217;s diverse scientific needs.<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-section-header | vf-u-margin__bottom--400\">\n  <h2 class=\"vf-section-header__heading\" \n    >\n  Contact  <\/h2>\n  <\/div>\n\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p><strong>Flow Cytometry Core Facility<\/strong><br>EMBL Heidelberg, Meyerhofstra\u00dfe 1, 69117 Heidelberg, Germany<\/p>\n\n\n\n<p>Tel: +49 6221 387-8341<\/p>\n\n\n\n<p><strong>Reservations, trainings and consultations:<\/strong><br>E-mail:&nbsp;<a href=\"mailto:fccf-team@embl.de\">fccf-team@embl.de<\/a><\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-14","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/pages\/14","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/comments?post=14"}],"version-history":[{"count":11,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/pages\/14\/revisions"}],"predecessor-version":[{"id":27821,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/pages\/14\/revisions\/27821"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/media?parent=14"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/flow-cytometry-heidelberg\/wp-json\/wp\/v2\/embl_taxonomy?post=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}