{"id":7170,"date":"2024-10-01T13:19:27","date_gmt":"2024-10-01T13:19:27","guid":{"rendered":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/?page_id=7170"},"modified":"2024-10-01T13:19:28","modified_gmt":"2024-10-01T13:19:28","slug":"bruker-skyscan-1272-microct","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/bruker-skyscan-1272-microct\/","title":{"rendered":"Bruker Skyscan 1272 microCT"},"content":{"rendered":"\n

Use of the Skyscan 1272 microCT has been heavily embedded into our 3D CLEM workflows in the EMCF. Samples are fixed, by means of chemical fixation or high pressure freezing\/freeze substitution, incubated with osmium tetroxide and heavy metals (introducing contrast to the sample) and embedded into a resin where it will be either imaged in the TEM or SEM. Once the resin block is scanned, we are left with a 3D map of the sample. We can measure the distance from the block surface to the ROI and trim away the material in-between bringing the ROI directly to the surface for imaging. <\/p>\n\n\n\n

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Reference:<\/p>\n\n\n\n

Karreman MA, Ruthensteiner B, Mercier L, Schieber NL, Solecki G, Winkler F, Goetz JG, Schwab Y, Find your way with X-Ray: Using microCT to correlate in vivo imaging with 3D electron microscopy<\/a>. Methods Cell Biol<\/em>. 2017:140:277-301.<\/p>\n","protected":false},"excerpt":{"rendered":"

Use of the Skyscan 1272 microCT has been heavily embedded into our 3D CLEM workflows in the EMCF. Samples are fixed, by means of chemical fixation or high pressure freezing\/freeze substitution, incubated with osmium tetroxide and heavy metals (introducing contrast to the sample) and embedded into a…<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-7170","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/7170","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/comments?post=7170"}],"version-history":[{"count":4,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/7170\/revisions"}],"predecessor-version":[{"id":15843,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/7170\/revisions\/15843"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/media?parent=7170"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/embl_taxonomy?post=7170"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}