{"id":4780,"date":"2022-12-27T10:48:29","date_gmt":"2022-12-27T10:48:29","guid":{"rendered":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/?page_id=4780"},"modified":"2023-01-20T10:39:39","modified_gmt":"2023-01-20T10:39:39","slug":"serial-block-face-sem-sbem","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/serial-block-face-sem-sbem\/","title":{"rendered":"Serial Block Face &#8211; SEM (SBEM)"},"content":{"rendered":"\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<p>The Serial Block Face \u2013 Scanning Electron Microscope (SBF-SEM or SBEM) is an automated instrument that allows 3D electron microscopy of large samples over days or weeks. It combines iterative imaging with a SEM and physical slicing of the sample with a diamond knife mounted on a microtome inside the SEM chamber. In the EMCF we have a Gatan 3view system mounted on a Zeiss Gemini 450 SEM. The x,y resolution that can be achieved is similar to that of a FIB-SEM (~5nm pixel size), but the z resolution is more limited (~30nm).<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<figure class=\"vf-figure wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2023\/01\/3View-2-768x1024.jpg\" alt=\"\" class=\"wp-image-5472\" width=\"280\" height=\"372\" srcset=\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2023\/01\/3View-2-768x1024.jpg 768w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2023\/01\/3View-2-225x300.jpg 225w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2023\/01\/3View-2-1152x1536.jpg 1152w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2023\/01\/3View-2.jpg 1512w\" sizes=\"auto, (max-width: 280px) 100vw, 280px\" \/><figcaption class=\"vf-figure__caption\">Zeiss Gemini 450 SEM with an integrated 3View system from Gatan.<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p>This instrument is typically used to image large tissues and small organisms up to several hundred microns in all dimensions (e.g. tissues or full <em>Platynereis dumerilii<\/em> larvae, sea urchin larvae, brain slices, mosquito gut), when high isotropic resolution is not necessary.\u00a0 Examples of samples recently acquired in the EMCF are mouse tissues (brain), sea urchin larvae, <em>Platynereis dumerilii <\/em>tissues, mosquito tissues, human leukocytes.<\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-full is-resized\"><img decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/12\/Sea-urchin.png\" alt=\"\" class=\"wp-image-5032\" width=\"-109\" height=\"-70\" srcset=\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/12\/Sea-urchin.png 720w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/12\/Sea-urchin-300x194.png 300w\" sizes=\"(max-width: 720px) 100vw, 720px\" \/><figcaption class=\"vf-figure__caption\">Sea urchin midgut, with exocrine pancreas-like cells segmented (Paganos et al., 2022)<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>The Serial Block Face \u2013 Scanning Electron Microscope (SBF-SEM or SBEM) is an automated instrument that allows 3D electron microscopy of large samples over days or weeks. It combines iterative imaging with a SEM and physical slicing of the sample with a diamond knife mounted on a microtome inside&hellip;<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-4780","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/4780","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/comments?post=4780"}],"version-history":[{"count":9,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/4780\/revisions"}],"predecessor-version":[{"id":5474,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/4780\/revisions\/5474"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/media?parent=4780"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/embl_taxonomy?post=4780"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}