{"id":273,"date":"2020-06-24T11:33:39","date_gmt":"2020-06-24T11:33:39","guid":{"rendered":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/?page_id=273"},"modified":"2022-12-27T09:26:04","modified_gmt":"2022-12-27T09:26:04","slug":"high-pressure-freezer-hpm-010","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/equipment\/high-pressure-freezer-hpm-010\/","title":{"rendered":"High Pressure Freezer &#8211; HPM010"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p><strong>High Pressure Freezing&nbsp; Machine<\/strong><\/p>\n\n\n\n<p>The HPM010 allows cryo-fixation and vitrification of specimens up to 200 \u00b5m in depth. In the EMCF we are routinely freezing bacterial and yeast cells, cultured mammalian cells, Drosophila embryos, nematodes, zebrafish embryos, marine organisms and several types of tissues. Adherent cells are typically grown on sapphire discs that can be used directly in the HPF machine. Other\u00a0 specimens are placed inside metal planchettes (with a depth between 40\u03bcm &#8211; 200\u03bcm), which are 3 mm in diameter and have a central cavity of 2 mm. To achieve vitrification, a cryoprotectant or filler is often added to the sample just before freezing. A second carrier with a flat surface is placed on top to protect the specimen. This sandwich is inserted into the machine and the specimen is exposed to 2000 bar of pressure within milliseconds while almost simultaneously being cooled down to liquid nitrogen temperature (-196<sup>o<\/sup>C). \u00a0 <\/p>\n\n\n\n<p><a href=\"https:\/\/ppms.embl.de\/planning\/?item=14\">Online Booking<\/a><\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image  | vf-figure--align vf-figure--align-inline-start   size-medium is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"225\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/06\/HPF-setup-copy-300x225.jpg\" alt=\"\" class=\"wp-image-1446\" srcset=\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/06\/HPF-setup-copy-300x225.jpg 300w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/06\/HPF-setup-copy-1024x768.jpg 1024w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/06\/HPF-setup-copy-768x576.jpg 768w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/06\/HPF-setup-copy-1536x1152.jpg 1536w, https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/06\/HPF-setup-copy-2048x1536.jpg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":123,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-273","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/273","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/comments?post=273"}],"version-history":[{"count":7,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/273\/revisions"}],"predecessor-version":[{"id":4696,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/273\/revisions\/4696"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/123"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/media?parent=273"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-json\/wp\/v2\/embl_taxonomy?post=273"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}