![\"image](\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60016118.jpg?itok=eWhSBDSK\")
\n \n \n Yannick Schwab<\/a>\n <\/h3>\n \n \n Team Leader and Head of Electron Microscopy Core Facility\n <\/p>\n \n \n \n \n \n \n \n
\n ORCID:<\/span>\n \n 0000-0001-8027-1836\n <\/a>\n <\/p>\n \n Edit\n <\/a>\n <\/article>\n <\/div>\n\n <\/div>\n<\/div>\n\n\n\n\n\n\n \n Overview <\/h2>\n <\/div>\n\n\n<\/div>\n<\/div>\n\n\n\n\n\nThe EMCF provides advanced expertise in cellular electron microscopy (EM) at room temperature: from sample preparation to image analysis and for a large variety of biological samples ranging from bacterial cells to (small) multicellular organisms. Our main focus is on ultrastructural analysis in 2D and 3D, immuno-EM and correlative multimodal imaging, combining EM with light microscopy (CLEM), and X-ray microscopy (CXEM) or both (CLXEM). Our staff can help you define optimal experimental conditions for your project.<\/p>\n\n\n\n
One of our strengths is the on-section CLEM technique, which tracks the signal of fluorescent proteins in resin sections with high precision. We have used this method for a number of studies (Groza et al. 2024<\/a>, Schreier et al. 2022<\/a>) and published a detailed step-by-step video tutorial<\/a> on this method.We are also hosting an international <\/a>course<\/a> covering the methodology every second year. <\/p>\n\n\n\nOur facility is also exploiting different volume EM techniques such as serial block face \u2013 scanning electron microscopy (SBF-SEM) and focused ion beam \u2013 scanning electron microscopy (FIB-SEM), to visualize the 3D ultrastructure of organelles at nanometer resolution across micron scales. For the precise targeting of the ROI in a 3D volume, we are routinely using correlative approaches combining light (see below) and X-ray imaging together with EM. The X-ray imaging is done in the facility with a tabletop system and when going for higher resolution in collaboration with Liz Duke on the EMBL beamline P14 at the PETRA III synchrotron in Hamburg.<\/p>\n\n\n\n
We are also involved in method development, which is heavily connected to our collaborations with groups here at the EMBL. An example of this is the development of a high-precision targeting methods for volume electron microscopy, together with the Ephrussi, Jechlinger, Leptin and Mahamid groups (Ronchi et al., 2021<\/a>). The strategy relies on fluorescence preservation during sample preparation and targeted trimming guided by confocal maps of the fluorescence signal in the resin block. Laser branding is used to create landmarks on the block surface to position the FIB-SEM acquisition. <\/p>\n\n<\/div>\n<\/div>\n\n\n\n\n\n![\"\"](\"https:\/\/www.embl.org\/groups\/electron-microscopy-core-facility\/wp-content\/uploads\/2022\/12\/JCB_202104069_Fig1.jpeg\")
Fluorescent molecules can be preserved within in many different specimens and targeted in the FIBSEM. Each row displays a confocal image with its corresponding FIBSEM image to the right of it. The far right panels show details of the structure of the organelles within the sample. Adapted from Ronchi et al., 2021.<\/figcaption><\/figure>\n\n\n\n
\n Overview <\/h2>\n <\/div>\n\n\n<\/div>\n<\/div>\n\n\n\n\n\nThe EMCF provides advanced expertise in cellular electron microscopy (EM) at room temperature: from sample preparation to image analysis and for a large variety of biological samples ranging from bacterial cells to (small) multicellular organisms. Our main focus is on ultrastructural analysis in 2D and 3D, immuno-EM and correlative multimodal imaging, combining EM with light microscopy (CLEM), and X-ray microscopy (CXEM) or both (CLXEM). Our staff can help you define optimal experimental conditions for your project.<\/p>\n\n\n\n
One of our strengths is the on-section CLEM technique, which tracks the signal of fluorescent proteins in resin sections with high precision. We have used this method for a number of studies (Groza et al. 2024<\/a>, Schreier et al. 2022<\/a>) and published a detailed step-by-step video tutorial<\/a> on this method.We are also hosting an international <\/a>course<\/a> covering the methodology every second year. <\/p>\n\n\n\nOur facility is also exploiting different volume EM techniques such as serial block face \u2013 scanning electron microscopy (SBF-SEM) and focused ion beam \u2013 scanning electron microscopy (FIB-SEM), to visualize the 3D ultrastructure of organelles at nanometer resolution across micron scales. For the precise targeting of the ROI in a 3D volume, we are routinely using correlative approaches combining light (see below) and X-ray imaging together with EM. The X-ray imaging is done in the facility with a tabletop system and when going for higher resolution in collaboration with Liz Duke on the EMBL beamline P14 at the PETRA III synchrotron in Hamburg.<\/p>\n\n\n\n
We are also involved in method development, which is heavily connected to our collaborations with groups here at the EMBL. An example of this is the development of a high-precision targeting methods for volume electron microscopy, together with the Ephrussi, Jechlinger, Leptin and Mahamid groups (Ronchi et al., 2021<\/a>). The strategy relies on fluorescence preservation during sample preparation and targeted trimming guided by confocal maps of the fluorescence signal in the resin block. Laser branding is used to create landmarks on the block surface to position the FIB-SEM acquisition. <\/p>\n\n<\/div>\n<\/div>\n\n\n\n\n\nFluorescent molecules can be preserved within in many different specimens and targeted in the FIBSEM. Each row displays a confocal image with its corresponding FIBSEM image to the right of it. The far right panels show details of the structure of the organelles within the sample. Adapted from Ronchi et al., 2021.<\/figcaption><\/figure>\n\n\n\n
The EMCF provides advanced expertise in cellular electron microscopy (EM) at room temperature: from sample preparation to image analysis and for a large variety of biological samples ranging from bacterial cells to (small) multicellular organisms. Our main focus is on ultrastructural analysis in 2D and 3D, immuno-EM and correlative multimodal imaging, combining EM with light microscopy (CLEM), and X-ray microscopy (CXEM) or both (CLXEM). Our staff can help you define optimal experimental conditions for your project.<\/p>\n\n\n\n
One of our strengths is the on-section CLEM technique, which tracks the signal of fluorescent proteins in resin sections with high precision. We have used this method for a number of studies (Groza et al. 2024<\/a>, Schreier et al. 2022<\/a>) and published a detailed step-by-step video tutorial<\/a> on this method.We are also hosting an international <\/a>course<\/a> covering the methodology every second year. <\/p>\n\n\n\n Our facility is also exploiting different volume EM techniques such as serial block face \u2013 scanning electron microscopy (SBF-SEM) and focused ion beam \u2013 scanning electron microscopy (FIB-SEM), to visualize the 3D ultrastructure of organelles at nanometer resolution across micron scales. For the precise targeting of the ROI in a 3D volume, we are routinely using correlative approaches combining light (see below) and X-ray imaging together with EM. The X-ray imaging is done in the facility with a tabletop system and when going for higher resolution in collaboration with Liz Duke on the EMBL beamline P14 at the PETRA III synchrotron in Hamburg.<\/p>\n\n\n\n We are also involved in method development, which is heavily connected to our collaborations with groups here at the EMBL. An example of this is the development of a high-precision targeting methods for volume electron microscopy, together with the Ephrussi, Jechlinger, Leptin and Mahamid groups (Ronchi et al., 2021<\/a>). The strategy relies on fluorescence preservation during sample preparation and targeted trimming guided by confocal maps of the fluorescence signal in the resin block. Laser branding is used to create landmarks on the block surface to position the FIB-SEM acquisition. <\/p>\n\n<\/div>\n<\/div>\n\n\n