{"id":136,"date":"2020-06-08T14:11:59","date_gmt":"2020-06-08T14:11:59","guid":{"rendered":"https:\/\/www.embl.org\/groups\/chemical-biology\/?page_id=136"},"modified":"2024-03-12T14:42:29","modified_gmt":"2024-03-12T14:42:29","slug":"screening","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/chemical-biology\/screening\/","title":{"rendered":"Screening"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>High Throughput Screening (HTS) allows a researcher to quickly conduct thousands of tests against a biological target through the use of automation (robotics,&nbsp;liquid handling devices,&nbsp;sensitive detectors), data processing and control software.<\/p>\n\n\n\n<p>Through this process one can rapidly identify active compounds (hits) which undergo further medical chemistry optimization to make them more suitable for development as drugs. A large number of projects at the core facility employ HTS as a part of project workflow.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Our expertise<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Assay development\n<ul class=\"wp-block-list\">\n<li>Selection of an appropriate assay readout<\/li>\n\n\n\n<li>Assessing the compatibility of the target assay with automation and&nbsp; high-throughput screening<\/li>\n\n\n\n<li>Developing and optimising assays for high-throughput screening<\/li>\n\n\n\n<li>Assisting in the design of secondary selectivity assays<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>Execution of HTS campaigns for biochemical, cell-based and protein-protein assays<\/li>\n\n\n\n<li>Follow up of hits with reporting of EC<sub>50<\/sub>\/IC<sub>50<\/sub>&nbsp;and solubility data<\/li>\n\n\n\n<li>Assay consultancy services (selection of assay technology, design, optimisation)<\/li>\n\n\n\n<li>Cell culture&nbsp;for cell-based assay targets<\/li>\n\n\n\n<li>Affinity studies for large protein and small molecules through&nbsp;Biacore surface plasmon resonance<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><a href=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/workflow.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"820\" height=\"700\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/workflow.jpg\" alt=\"\" class=\"wp-image-139\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/workflow.jpg 820w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/workflow-300x256.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/workflow-768x656.jpg 768w\" sizes=\"auto, (max-width: 820px) 100vw, 820px\" \/><\/a><figcaption class=\"vf-figure__caption\">Typical project workflow<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h2 class=\"wp-block-heading\" id=\"facilities\">Facilities, services and equipment<\/h2>\n\n\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-49667f6a-cfc2-41af-8d4f-21abb7efa0d3\" data-vf-js-location-nearest-activation-target=\"\">Automated platform<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-b37cb2db-e2b4-415e-ae38-fe2694f276c9\" data-vf-js-location-nearest-activation-target=\"\">Biacore T200<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-caafa368-62ec-49e1-9dd5-935a954c68af\" data-vf-js-location-nearest-activation-target=\"\">Cell culture suite<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-d101288e-7476-468f-b508-edb4fa9d61c4\" data-vf-js-location-nearest-activation-target=\"\">Liquid handling<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-eec8e705-d1b8-41c7-91d5-7ad53dd7d2be\" data-vf-js-location-nearest-activation-target=\"\">Multilabel readers<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-49667f6a-cfc2-41af-8d4f-21abb7efa0d3\"><h2 class=\"vf-u-sr-only\">Automated platform<\/h2>\n<p><\/p>\n\n\n\n<p>The facility has a fully integrated automated platform for running biochemical and cell-based screening campaigns. At the core of the platform is a Janus integrator fitted with a gripper tool which can access the Cytomat, de-lid cell plates, transfer to the Multidrop for reagent addition and return the cell plates to the cell incubator or the EnVision microplate reader. Furthermore, the devices on the platform can be used independently or as standalone units:<\/p>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"259\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Janus-crop_s-300x259.jpg\" class=\"vf-summary__image\" alt=\"\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Janus-crop_s-300x259.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Janus-crop_s.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    Janus (PerkinElmer)\n    <\/h3>\n\n  <article class=\"vf-summary vf-summary--news\">\n<p class=\"vf-summary__text\">(equipped with a 384 channel head) used for the transfer of fluids in plate replication, reformatting of e.g. 96 to 384 well, IC<sub>50<\/sub>\/EC<sub>50<\/sub>\u00a0and assay plate preparation.<\/p>\n<\/article>\n<\/article>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"162\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Multi_drop_0393_s-300x162.jpg\" class=\"vf-summary__image\" alt=\"\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Multi_drop_0393_s-300x162.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Multi_drop_0393_s.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    MultiDrop Combi (ThermoFisher)\n    <\/h3>\n\n  <article class=\"vf-summary vf-summary--news\">\n<article class=\"vf-summary vf-summary--news\">\n<p class=\"vf-summary__text\">Fast bulk dispenser for reagents and cells. Can dispense reagents in the range of 0.5 \u00b5l to 2500 \u00b5l into 96, 384 and 1536 well plates.<\/p>\n<\/article>\n<\/article>\n<\/article>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"252\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Cytomat-0455_s-300x252.jpg\" class=\"vf-summary__image\" alt=\"\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Cytomat-0455_s-300x252.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Cytomat-0455_s.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    Cytomat 10C Incubator (ThermoFisher)\n    <\/h3>\n\n  <article class=\"vf-summary vf-summary--news\">\n<article class=\"vf-summary vf-summary--news\">\n<article class=\"vf-summary vf-summary--news\">\n<p class=\"vf-summary__text\">Features rotatable plate carousels. Capacity for 210 multi-well cell assay plates. Automation access from the rear or front in stand-alone mode.<\/p>\n<\/article>\n<\/article>\n<\/article>\n<\/article>\n\n\n\n<article class=\"vf-summary \">\n    <h3 class=\"vf-summary__title\">\n    \n    EnVisionTM Xcite (PerkinElmer)\n    <\/h3>\n\n  <p class=\"vf-summary__text\">Single detector multilabel reader with 2 channel injection module.<\/p>\n<\/article>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-b37cb2db-e2b4-415e-ae38-fe2694f276c9\"><h2 class=\"vf-u-sr-only\">Biacore T200<\/h2>\n<p><\/p>\n\n\n\n<p>Our Biacore T200 supports the use of 96- and 384-well microplates. The use of all four flow cells allows four interactions to be simultaneously monitored.<\/p>\n\n\n\n<p>Surface Plasmon Resonance (SPR) for ligand-binding assays<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Kinetics\/affinity characterisation<\/li>\n\n\n\n<li>Kinetics\/affinity screening<\/li>\n\n\n\n<li>Single-cycle kinetics (fast runs without regeneration)<\/li>\n\n\n\n<li>LMW interaction analysis<\/li>\n\n\n\n<li>Fragment screening<\/li>\n\n\n\n<li>Concentration analysis<\/li>\n\n\n\n<li>Calibration-free concentration analysis<\/li>\n\n\n\n<li>Thermodynamics<\/li>\n<\/ul>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"820\" height=\"284\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/biacore.jpg\" alt=\"\" class=\"wp-image-260\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/biacore.jpg 820w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/biacore-300x104.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/biacore-768x266.jpg 768w\" sizes=\"auto, (max-width: 820px) 100vw, 820px\" \/><figcaption class=\"vf-figure__caption\">Loading of 96 well microplate on the Biacore.<br><br><br><\/figcaption><\/figure>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-caafa368-62ec-49e1-9dd5-935a954c68af\"><h2 class=\"vf-u-sr-only\">Cell culture suite<\/h2>\n<p><\/p>\n\n\n\n<p>With access to a dedicated safety level S1 cell culture suite, the facility has experience in handling transient and stable mammalian cells as well as patient-derived cells to supply cell-based HTS campaigns run at the CBCF.<\/p>\n\n\n\n<p>We require two vials of your cell lines which are Mycoplasma free, for which we determine the growth rates, bulk up and bank several vials to support your project.<\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><a href=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0602_m.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"820\" height=\"636\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0602_m.jpg\" alt=\"\" class=\"wp-image-265\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0602_m.jpg 820w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0602_m-300x233.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0602_m-768x596.jpg 768w\" sizes=\"auto, (max-width: 820px) 100vw, 820px\" \/><\/a><figcaption class=\"vf-figure__caption\">Cell culture hood<\/figcaption><\/figure>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-d101288e-7476-468f-b508-edb4fa9d61c4\"><h2 class=\"vf-u-sr-only\">Liquid handling<\/h2>\n<p><\/p>\n\n\n\n<p>In addition to the devices on the automation platform, the facility has two stand-alone liquid handling devices:<\/p>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"201\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/FlexDrop_0464_s-300x201.jpg\" class=\"vf-summary__image\" alt=\"\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/FlexDrop_0464_s-300x201.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/FlexDrop_0464_s.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    PerkinElmer FlexDropTM IV EXi\n    <\/h3>\n\n  <article class=\"vf-summary vf-summary--news\">\n<p class=\"vf-summary__text\">(Fast bulk dispenser for reagents and cells)<br \/>\nCan dispense up to 4 reagents in the range of 200nl to 2ml into 96, 384 and 1536 well plates.<br \/>\nCan handle up to 50 microplates in the various densities.<\/p>\n<\/article>\n<\/article>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"300\" height=\"176\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/MultiPROBE_0528_s-300x176.jpg\" class=\"vf-summary__image\" alt=\"\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/MultiPROBE_0528_s-300x176.jpg 300w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/MultiPROBE_0528_s.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    PerkinElmer MultiProbe\u00ae II Plus Ex 4-Channel Pipettor\n    <\/h3>\n\n  <article class=\"vf-summary vf-summary--news\">\n<p class=\"vf-summary__text\">Fitted with 4 varispan tips and used primarily for dissolving compounds, preparation of master plates, cherry picking and assay development.<\/p>\n<\/article>\n<\/article>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-eec8e705-d1b8-41c7-91d5-7ad53dd7d2be\"><h2 class=\"vf-u-sr-only\">Multilabel readers<\/h2>\n<p><\/p>\n\n\n\n<p>The facility has 4 stand-alone readers capable covering a large spectrum of detection technologies:<\/p>\n\n\n\n<article class=\"vf-summary vf-summary--has-image\">\n  <img decoding=\"async\" width=\"224\" height=\"300\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/EnVision_0481_s-224x300.jpg\" class=\"vf-summary__image\" alt=\"\" style=\"width: 180px; height: auto; border: 1px solid #d0d0ce\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/EnVision_0481_s-224x300.jpg 224w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/EnVision_0481_s.jpg 350w\" sizes=\"auto, (max-width: 224px) 100vw, 224px\" \/>  <h3 class=\"vf-summary__title\">\n    \n    EnVisionTM HTS (PerkinElmer)\n    <\/h3>\n\n  <ul>\n<li>Fast dual detector reader fitted with plate stackers (50 plate maximum capacity)<\/li>\n<li>Absorbance<\/li>\n<li>Fluorescence intensity<\/li>\n<li>TR-FRET<\/li>\n<li>AlphaScreenTM (Amplified Luminescent Proximity Homogeneous Assay)<\/li>\n<li>Enhanced luminescence<\/li>\n<\/ul>\n<\/article>\n\n\n\n<article class=\"vf-summary \">\n    <h3 class=\"vf-summary__title\">\n    \n    Safire 2 (Tecan)\n    <\/h3>\n\n  <p class=\"vf-summary__text\">Monochromator plate reader allowing the use of any combination of excitation and emission wavelengths and supporting following technologies:<\/p>\n<ul>\n<li>Absorbance<\/li>\n<li>Fluorescence intensity<\/li>\n<\/ul>\n<\/article>\n\n\n\n<article class=\"vf-summary \">\n    <h3 class=\"vf-summary__title\">\n    \n    MicroBeta2 LumiJet (PerkinElmer)\n    <\/h3>\n\n  <p class=\"vf-summary__text\">Liquid scintillation and luminescence counter.<\/p>\n<ul>\n<li>Beta or gamma counter<\/li>\n<li>Scintillation proximity assays (SPA)<\/li>\n<li>Flash or glow luminescence<\/li>\n<li>Compatible with \u00a024, 96 or 384 microplates<\/li>\n<\/ul>\n<\/article>\n\n\n\n<article class=\"vf-summary \">\n    <h3 class=\"vf-summary__title\">\n    \n    NEPHELOstar (BMG Labtech)\n    <\/h3>\n\n  <p class=\"vf-summary__text\">Laser-based microplate nephelometer reader for detection of particles in solution. Applications include:<\/p>\n<ul>\n<li>Compound solubility testing in 96- and 384-well plates<\/li>\n<li>Monitoring microbial growth and protein binding<\/li>\n<\/ul>\n<\/article>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Contact:<\/h3>\n\n\n\n<p><strong>Chemical Biology Core Facility<\/strong><br>EMBL Heidelberg, Meyerhofstra\u00dfe 1, 69117 Heidelberg, Germany<\/p>\n\n\n\n<p><strong>Phone:<\/strong>&nbsp;+49 6221 387-8303<br><strong>Fax:<\/strong>&nbsp;+49 6221 387-8306<br><strong>Email:<\/strong>&nbsp;<a href=\"https:\/\/www.embl.org\/groups\/chemical-biology\/contact\/\" data-type=\"page\" data-id=\"3962\">General enquiries<\/a><\/p>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h2 class=\"wp-block-heading\">Screening library<\/h2>\n\n\n\n<p>Our diversity oriented screening library is composed of 110,000 compounds. This library was selected from more than 5 million structures that were collected \u201cvirtually\u201d and put through a rigorous filtering process. In addition, they were analysed on the basis of their \u2018scaffold\u2019 content to ensure the optimum coverage of chemical space \u2013 each compound structure was virtually fragmented into its core (the scaffold) and side chains.<\/p>\n\n\n\n<p>The scaffold-based approach provides high information content for screening:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The structural space around each scaffold is covered appropriately;<\/li>\n\n\n\n<li>Any hit compounds from a high-throughput screen can be rapidly followed up by selecting similar compounds to enable initial structure-activity relationships to be discerned;<\/li>\n\n\n\n<li>This will help in the prioritisation of the hit compounds for further medicinal chemistry optimisation.<\/li>\n<\/ul>\n\n\n\n<p>Selected compounds were finally checked for drug-likeness, structural and shape diversity, novelty, and compliance with medicinal chemistry requirements. Individual compound selection was done by picking representative compounds around selected scaffolds.<\/p>\n\n\n\n<p>We offer access to our compound collection for screening at the facility or externally in your laboratory.<\/p>\n\n\n\n<p>The compounds are prepared at 10mM in 100% DMSO and stored at -20<sup>o<\/sup>C. Batches of assay ready plates are typically produced in advance of the screening campaigns. This reduces the cost of processing the assay plates and means initiation of HTS is not dependent on availability of resources.<\/p>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"350\" height=\"210\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0480_s.jpg\" alt=\"\" class=\"wp-image-162\" srcset=\"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0480_s.jpg 350w, https:\/\/www.embl.org\/groups\/chemical-biology\/wp-content\/uploads\/2020\/06\/Core-Facility_0480_s-300x180.jpg 300w\" sizes=\"auto, (max-width: 350px) 100vw, 350px\" \/><figcaption class=\"vf-figure__caption\">An array of frozen compound stocks in a storage rack<\/figcaption><\/figure>\n\n\n\n<div class=\"vf-box vf-box--normal vf-box-theme--quinary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n                Diversity oriented screening library                  <\/h3> \n        <ul class=\"rich-text block-editor-rich-text__editable\" role=\"textbox\" contenteditable=\"true\" aria-multiline=\"true\" aria-label=\"Write list\u2026\">\n<li>110,000 compounds.<\/li>\n<\/ul>\n<\/div>\n\n\n<div class=\"vf-box vf-box--normal vf-box-theme--quinary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n                Covalent Binder libraries                  <\/h3> \n        <ul>\n<li>15.000 covalent binders from Enamine<\/li>\n<li>800 covalent binders (fragment size) from ChemDiv<\/li>\n<\/ul>\n<\/div>\n\n\n<div class=\"vf-box vf-box--normal vf-box-theme--quinary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n                Annotated drug libraries                  <\/h3> \n        <ul class=\"rich-text block-editor-rich-text__editable\" role=\"textbox\" contenteditable=\"true\" aria-multiline=\"true\" aria-label=\"Write list\u2026\">\n<li>NIH<\/li>\n<li>Microsource<\/li>\n<li>Selleck Bioactive compounds II<\/li>\n<li>Sigma (Pfizer library)<\/li>\n<li>Fragment library (100mM in 100% DMSO)<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-136","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/pages\/136","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/comments?post=136"}],"version-history":[{"count":181,"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/pages\/136\/revisions"}],"predecessor-version":[{"id":13237,"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/pages\/136\/revisions\/13237"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/media?parent=136"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/chemical-biology\/wp-json\/wp\/v2\/embl_taxonomy?post=136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}