{"id":163,"date":"2020-06-25T09:44:52","date_gmt":"2020-06-25T09:44:52","guid":{"rendered":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-service\/?page_id=163"},"modified":"2021-08-10T12:39:18","modified_gmt":"2021-08-10T12:39:18","slug":"microscope-automation","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/microscope-automation\/","title":{"rendered":"Microscope automation"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">The ALMF offers support for microscope automation &#8211; Adaptive Feedback Microscopy<\/h3>\n\n\n\n<p>Adaptive Feedback Microscopy is a powerful methodology for performing high-content microscopy experiments in an automated manner. Initially acquired images are automatically analyzed and coordinates of the features of interest are communicated back to trigger follow-up acquisitions.<\/p>\n\n\n\n<p>In particular, we offer:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Establishing automated acquisition protocols, including automated scanning of multi-well plates.<\/li><li>Microscope experiments with online image analysis, where objects of interest are detected automatically and a complex acquisition protocol is launched for these objects.<br>Example: <a href=\"https:\/\/europepmc.org\/abstract\/MED\/33057197\" target=\"_blank\" rel=\"noreferrer noopener\">Fuqua et al. Nature (2020)<\/a> <\/li><li>To this end we have developed software interfaces between confocal microscopes (Leica SP5, Zeiss Zen Black and Zen Blue, Olympus FV3000) that establish a feedback loop between popular image analysis packages (Cellprofiler, <a href=\"https:\/\/fiji.sc\" target=\"_blank\" rel=\"noreferrer noopener\">Fiji<\/a>) and the microscope software.<\/li><li>Experiments involving liquid handling on the microscope stage (e.g. adding a drug) using automated dispensing systems.<\/li><\/ul>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n<div class=\"vf-links\">\n      <h3 class=\"vf-links__heading\">HTM Services<\/h3>\n    <ul class=\"vf-links__list vf-list\">\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/\">\n        High Throughout Microscopy      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/rnai-expertise\/\">\n        RNAi expertise      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/rnai-libraries\/\">\n        RNAi libraries      <\/a>\n      <\/li>\n    <li class=\"vf-list__item\">\n          <a class=\"vf-list__link\" href=\"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/supported-formats\/\">\n        Supported formats      <\/a>\n      <\/li>\n  <\/ul>\n\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":153,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-163","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/163","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/comments?post=163"}],"version-history":[{"count":15,"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/163\/revisions"}],"predecessor-version":[{"id":1562,"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/163\/revisions\/1562"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/pages\/153"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/media?parent=163"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/wp-json\/wp\/v2\/embl_taxonomy?post=163"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}