{"id":157,"date":"2020-06-25T09:44:10","date_gmt":"2020-06-25T09:44:10","guid":{"rendered":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-service\/?page_id=157"},"modified":"2021-08-10T12:41:12","modified_gmt":"2021-08-10T12:41:12","slug":"rnai-libraries","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/rnai-libraries\/","title":{"rendered":"RNAi libraries"},"content":{"rendered":"\n
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Ambion – Silencer select<\/a><\/h3>\n\n\n\n

Silencer select siRNAs have LNA\u00ae chemical modifications which enhance guide strand bias and reduce off target effects by up to 90 %.<\/p>\n\n\n\n

2 \/ 3 siRNAs are guaranteed to achieve >70% reduction in target mRNA levels.<\/p>\n\n\n\n

Kinase<\/td>710 genes <\/td>=<\/td>2 130 silencer select siRNAs (3 x \/ gene)<\/td><\/tr>
Phosphatase<\/td>298 genes  <\/td>=<\/td>894 silencer select siRNAs (3 x \/ gene)<\/td><\/tr>
Ubiquitin<\/td>1 346 genes<\/td>=<\/td>2 692 silencer select siRNAs (2 x \/ gene)<\/td><\/tr>
Mitosis<\/td>1 137 genes<\/td>=<\/td>2 274 silencer select siRNAs (2 x \/ gene)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Ambion – Silencer<\/a><\/h3>\n\n\n\n

Silencer siRNAs have no chemical modifications. 2 \/ 3 siRNAs are guaranteed to achieve >70% reduction in target mRNA levels.<\/p>\n\n\n\n

Kinase<\/td>710 genes <\/td>=<\/td>2 130 silencer select siRNAs (3 x \/ gene)<\/td><\/tr>
Druggable<\/td>5 940 genes  <\/td>=<\/td>17 820 silencer select siRNAs (3 x \/ gene)<\/td><\/tr>
Genome-wide<\/td>23 686 genes<\/td>~<\/td>52 000 silencer select siRNAs (2-3 x \/ gene)<\/td><\/tr>
Mitosis<\/td>1 266 genes<\/td>=<\/td>1 266 silencer select siRNAs (1 x \/ gene)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Qiagen<\/h3>\n\n\n\n
Secretion<\/td>1 552 genes <\/td>=<\/td>1 652 silencer select siRNAs (1-2 x \/ gene) <\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/div>\n\n\n
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HTM Services<\/h3>\n