{"id":155,"date":"2020-06-25T09:43:50","date_gmt":"2020-06-25T09:43:50","guid":{"rendered":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-service\/?page_id=155"},"modified":"2021-08-10T12:12:08","modified_gmt":"2021-08-10T12:12:08","slug":"rnai-expertise","status":"publish","type":"page","link":"https:\/\/www.embl.org\/groups\/advanced-light-microscopy-core-facility\/services\/high-throughput-microscopy\/rnai-expertise\/","title":{"rendered":"RNAi expertise"},"content":{"rendered":"\n
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We have developed a number of RNAi transfection protocols (Erfle et al<\/em>.<\/a>), which can be also used for plasmids<\/strong> and CRISPR guide RNAs<\/strong> (Ser\u00e7in et al<\/a>).<\/p>\n\n\n\n

These protocols are based on solid phase reverse transfection and can be used on multi-well plates or LabTek slides. The ALMF provides equipment for coating such plates or LabTeks.<\/p>\n\n\n\n

We can offer support with:<\/p>\n\n\n\n

  1. siRNA design<\/li>
  2. Selection<\/a> of available or customized siRNA libraries<\/li>
  3. siRNA plate layout<\/li>
  4. RNAi transfection<\/li>
  5. Bioinformatic analysis of siRNA sequences<\/li><\/ol>\n\n\n\n

    In addition, we run siRNA design courses<\/a> (internal only) on a regular basis.<\/p>\n\n<\/div>\n<\/div>\n\n\n

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    HTM Services<\/h3>\n