8 April - 12 April 2024
scATAC-seq: attacking open chromatin in single cells
Vladimir Benes, Ulrike Litzenburger, Judith Zaugg
ATAC Seq stands for Assay of Transposase Accessible Chromatin and is a widespread method to profile open chromatin on a genome scale The goal of this introductory course is to teach the entire workflow of a single cell and bulk ATAC Seq experiment including library preparation sequencing data processing and downstream analysis by addressing a real biological question that will be answered at the end of the course... OverviewSpeakersProgrammePractical informationSponsorsMedia kit Overview Course overview ATAC-Seq stands for Assay of Transposase Accessible Chromatin and is a widespread method to profile open chromatin on a genome-scale. The goal of this introductory course is to teach the entire workflow of a single cell ATAC-Seq experiment including library preparation, sequencing, data processing, and downstream analysis by addressing a real biological question that will be answered at the end of the course. We will start with the experimental single cell ATAC-Seq protocol, highlighting the critical steps, potential pitfalls, and the most up-to-date tweaks to improve the quality of the results. After sequencing the samples we will go through the basic quality control and preprocessing steps using multiple analysis tools for next generation sequencing data. Finally, we will perform different types of...
Course
EMBL Heidelberg
Closed