{"id":32270,"date":"2022-03-15T11:30:50","date_gmt":"2022-03-15T11:30:50","guid":{"rendered":"https:\/\/www.embl.org\/ells\/?post_type=learninglab&#038;p=32270"},"modified":"2022-05-11T09:48:07","modified_gmt":"2022-05-11T09:48:07","slug":"fluorescent-recombinants-molecular-cloning","status":"publish","type":"learninglab","link":"https:\/\/www.embl.org\/ells\/training\/fluorescent-recombinants-molecular-cloning\/","title":{"rendered":"Fluorescent recombinants &#8211; making sense of molecular cloning"},"content":{"rendered":"\n        <div class=\"vf-tabs\">\n      <ul class=\"vf-tabs__list\" data-vf-js-tabs>\n                <li class=\"vf-tabs__item\">\n          <a class=\"vf-tabs__link\" href=\"#vf-tabs__section--1\">Overview<\/a>\n        <\/li>\n                <li class=\"vf-tabs__item\">\n          <a class=\"vf-tabs__link\" href=\"#vf-tabs__section--2\">About the educational resource <\/a>\n        <\/li>\n                <li class=\"vf-tabs__item\">\n          <a class=\"vf-tabs__link\" href=\"#vf-tabs__section--3\">About the workshop <\/a>\n        <\/li>\n                <li class=\"vf-tabs__item\">\n          <a class=\"vf-tabs__link\" href=\"#vf-tabs__section--4\">Organiser<\/a>\n        <\/li>\n                <li class=\"vf-tabs__item\">\n          <a class=\"vf-tabs__link\" href=\"#vf-tabs__section--5\">Requirements &#038; certificate<\/a>\n        <\/li>\n                <li class=\"vf-tabs__item\">\n          <a class=\"vf-tabs__link\" href=\"#vf-tabs__section--6\">Application<\/a>\n        <\/li>\n              <\/ul>\n    <\/div>\n    \n        <div class=\"vf-tabs-content\" data-vf-js-tabs-content>\n            <section class=\"vf-tabs__section\" id=\"vf-tabs__section--1\">\n        <h2>Overview<\/h2>\n                  <p><p><span style=\"font-weight: 400;\">With the discovery of restriction enzymes in the 1970s began the rise of molecular cloning techniques to create recombinant DNA molecules. Ever since they have become standard laboratory techniques that allow scientists to gain insights into the function of genes and the proteins they encode. If you are a STEM teacher interested in a new educational tool that introduces students to molecular cloning,\u00a0 join the online TeachingBASE workshop \u201cFluorescent recombinants &#8211; making sense of molecular cloning\u201d on Wednesday,\u00a0 18 May 2022, run by EMBL\u2019s <\/span><a href=\"http:\/\/www.embl.org\/ells\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">European Learning Laboratory for the Life Sciences<\/span><\/a> <a href=\"http:\/\/www.embl.org\/ells\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">(ELLS)<\/span><\/a><span style=\"font-weight: 400;\">. The workshop&#8217;s working language is English.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">ELLS offers workshops on two additional molecular biology resources. For more information, visit the workshops\u2019 respective web pages.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\"><a href=\"https:\/\/www.embl.org\/ells\/learninglab\/copy-that-pcr-paper-based\/\" target=\"_blank\" rel=\"noopener\">\u201cCopy that! &#8211; making sense of PCR\u201d (paper-based)<\/a> on 6 April 2022, 18:00- 20:00 CET<\/span><\/p>\n<p><span style=\"font-weight: 400;\"><a href=\"https:\/\/www.embl.org\/ells\/learninglab\/copy-that-pcr-computer-based\/\" target=\"_blank\" rel=\"noopener\">\u201cCopy that! &#8211; making sense of PCR\u201d (computer-based)<\/a> on 12 May 2022, 18:00- 20:00 CET<\/span><\/p>\n<\/p>\n        \n        <!-- Nested tabs-->\n        \n              <\/section>\n            <section class=\"vf-tabs__section\" id=\"vf-tabs__section--2\">\n        <h2>About the educational resource <\/h2>\n                  <p><p><span style=\"font-weight: 400;\">\u201cFluorescent recombinants &#8211; making sense of molecular cloning\u201d is a computer-based, hands-on teaching resource that guides students through the process of designing a DNA cloning experiment to find the subcellular location of a yeast protein. To achieve this goal, students follow step-by-step the process of fusing a gene of interest with another gene encoding the green fluorescent protein (GFP) and verifying successfully generated recombinant clones. By doing so, students become familiar with the concepts of cloning vectors, restriction digestion, ligation, bacterial transformation and screening for clones.\u00a0 In a final step, students evaluate the outcome of the experiment by interpreting the results visualised on sample pictures.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">While some of the classroom activities are paper-based, the primary part of the resource focuses on computer-based activities to tackle the tasks. The classroom activities will take 120-150 minutes to complete.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">The resource is designed to teach molecular cloning to students aged 16-20 years in the classroom or remotely. Students should be familiar with the basic concepts of DNA. It will also be beneficial if they already know some of the basics of molecular cloning or related topics such as plasmids, enzymatic reactions, restriction digestion or bacterial transformation.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">To equip students with the information they need to complete the activities, the resource provides worksheets, fact sheets, a step-by-step tutorial for the software \u201cApE- A plasmid Editor\u201d, and the files with the DNA sequences of the genes and the vectors.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">By using this resource, students will<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">follow authentic procedures carried out in scientific laboratories<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">design a cloning experiment\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">become comfortable using an online tool for plasmid editing<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">analyse a<\/span><span style=\"font-weight: 400;\">nd discuss the <\/span><span style=\"font-weight: 400;\">success of the cloning strategy they chose to follow<\/span><\/li>\n<\/ul>\n<\/p>\n        \n        <!-- Nested tabs-->\n        \n              <\/section>\n            <section class=\"vf-tabs__section\" id=\"vf-tabs__section--3\">\n        <h2>About the workshop <\/h2>\n                  <p><p><span style=\"font-weight: 400;\">This workshop is for educators who teach any level of upper secondary school biology and is particularly suitable for those teaching students aged 16 and older. The workshop will take place online via Zoom on <\/span><span style=\"font-weight: 400;\">Wednesday,\u00a0 18 May <\/span><span style=\"font-weight: 400;\">2022, from 18:00 &#8211; 20:00 CET.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">We will start this workshop with a short introduction to the <\/span><span style=\"font-weight: 400;\">\u201cFluorescent recombinants &#8211; making sense of molecular cloning\u201d <\/span><span style=\"font-weight: 400;\">educational resource. Participants will then get a chance to try out the learning material themselves, working in small teams to explore the resource from the perspectives of students and educators.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">By the end of the workshop, participants will<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">be familiar with a new resource for teaching biology in secondary school<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">feel confident in using the new material in the classroom<\/span><\/li>\n<\/ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">feel competent in using the ApE tool in the classroom<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">expand their network of international teachers<\/span><\/li>\n<\/ul>\n<\/p>\n        \n        <!-- Nested tabs-->\n        \n              <\/section>\n            <section class=\"vf-tabs__section\" id=\"vf-tabs__section--4\">\n        <h2>Organiser<\/h2>\n                  <p><p><a href=\"http:\/\/www.embl.org\/ells\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">European Learning Laboratory for the Life Sciences<\/span><\/a> <a href=\"http:\/\/www.embl.org\/ells\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">(ELLS)<\/span><\/a><\/p>\n<p><span style=\"font-weight: 400;\">This training opportunity is organised by <\/span><a href=\"https:\/\/www.embl.org\/\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">EMBL<\/span><\/a><span style=\"font-weight: 400;\">\u2018s science education department ELLS. The ELLS programmes convey complex, cutting-edge topics in life science research in an exciting and insightful way, fostering the discovery of current research trends, the scientific method, and scientific career paths.\u00a0 We offer continued professional development training for teachers and develop educational resources based on EMBL science. Our programmes for school students and young learners explore the advances in scientific research inside and outside the classroom. Visit <\/span><a href=\"http:\/\/www.embl.org\/ells\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">our website<\/span><\/a><span style=\"font-weight: 400;\"> to learn more about our activities.<\/span><\/p>\n<\/p>\n        \n        <!-- Nested tabs-->\n        \n              <\/section>\n            <section class=\"vf-tabs__section\" id=\"vf-tabs__section--5\">\n        <h2>Requirements &#038; certificate<\/h2>\n                  <p><p><b>Requirements<\/b><\/p>\n<p><span style=\"font-weight: 400;\">We recommend a minimum English language level of B2 according to the Common European Framework of Reference for Languages to get the most out of the TeachingBASE workshop. Please consult <\/span><a href=\"https:\/\/rm.coe.int\/CoERMPublicCommonSearchServices\/DisplayDCTMContent?documentId=090000168045bb52\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">this self-assessment chart<\/span><\/a><span style=\"font-weight: 400;\"> if you are unsure about your language skills.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">The TeachingBASE workshop will take place on the Zoom meeting tool. To join the workshop, participants will need to have access to a computer with a stable internet connection, audio connection as well as a camera. Click <\/span><a href=\"https:\/\/ecampus.embl.de\/pluginfile.php\/24812\/mod_resource\/content\/1\/Zoom_tutorial_participants.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">here<\/span><\/a><span style=\"font-weight: 400;\"> for more information on how to install and use Zoom.\u00a0<\/span><\/p>\n<p><b>Certification<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Participants will receive a certificate of attendance upon completion of the TeachingBASE workshop.<\/span><\/p>\n<\/p>\n        \n        <!-- Nested tabs-->\n        \n              <\/section>\n            <section class=\"vf-tabs__section\" id=\"vf-tabs__section--6\">\n        <h2>Application<\/h2>\n                  <p><p><b>Terms and conditions<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Please make yourself familiar with the <\/span><a href=\"https:\/\/www.embl.org\/ells\/terms-and-conditions\/\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">terms and conditions<\/span><\/a><span style=\"font-weight: 400;\"> for participating in our workshops and on data protection. Your agreement towards these terms and conditions is required before you can continue with applying for the workshop.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">As we anticipate the ELLS TeachingBASE workshop to be very popular, we ask participants to only apply if they are certain that they can attend. In case you find yourself unable to attend after having received our confirmation email, please notify us as soon as possible by email but no later than one week before the start of the workshop.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Please note that cancellations and non-attendance might jeopardise the viability of the workshop for other participants.<\/span><\/p>\n<p><b>Application<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Please ensure that you have read all TeachingBASE workshop information on this page before applying and are confident that you are available on the workshop date.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">To apply, please fill in the<\/span><a href=\"https:\/\/www.surveymonkey.de\/r\/TBw_3\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\"> application form<\/span><\/a><span style=\"font-weight: 400;\">. The workshop spots are distributed on a first-come-first-serve basis. Priority will be given to educators who teach secondary school biology. All accepted applicants will be contacted within 7 days of application submission with further information about the workshop. The workshop will only take place if a minimum number of 15 participants have registered. <\/span><\/p>\n<\/p>\n        \n        <!-- Nested tabs-->\n        \n              <\/section>\n          <\/div>\n    <!--\/vf-tabs-content-->\n      <!--\/vf-grid-->\n  ","protected":false},"excerpt":{"rendered":"<p>Getting familiar with educational resource which teaches DNA cloning using the computer<\/p>\n","protected":false},"featured_media":32306,"parent":0,"menu_order":0,"template":"","class_list":["post-32270","learninglab","type-learninglab","status-publish","has-post-thumbnail","hentry","llabs-type-teachingbase-workshop","llabs-format-virtual","llabs-location-online","topic-area-methods-in-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/training\/32270","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/training"}],"about":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/types\/learninglab"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media\/32306"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media?parent=32270"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}