{"id":29378,"date":"2015-10-05T20:05:57","date_gmt":"2015-10-05T18:05:57","guid":{"rendered":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/"},"modified":"2021-06-18T14:46:54","modified_gmt":"2021-06-18T14:46:54","slug":"step-5-structure-of-gfp","status":"publish","type":"teachingbase","link":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/?lang=sv","title":{"rendered":"Steg 5: GFP struktur"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-74c95bab-501c-4bc2-bc5c-4bd79038234c\">\u00d6versikt<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-1a6bebfa-8428-433f-9cfc-6506b7154097\">Din uppgift<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-4c7c5382-9083-47d1-8a64-9c108e184908\">PDBe<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-04943c3b-3a86-4fec-b356-e6cb9688f09d\">Fr\u00e5gor<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-d7a4ed6c-a6b9-4bbf-9b2a-28eb7cacd6a2\">Aktivitetsnavigering<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-74c95bab-501c-4bc2-bc5c-4bd79038234c\"><h2>\u00d6versikt<\/h2>\n<p>I den sista delen i aktiviteten ska vi anv\u00e4nda Protein Data Bank Europe (PDBe) f\u00f6r att visualisera en interaktiv tre-dimensionell struktur av GFP och leka lite med den.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-1a6bebfa-8428-433f-9cfc-6506b7154097\"><h2>Din uppgift<\/h2>\n<p><strong>Forts\u00e4tt som beskrivs nedan:<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\"><li>Klicka p\u00e5 fliken &#8220;PDBe&#8221;.<\/li><li>S\u00f6k efter n\u00e5gon av <strong>Green Fluorescent Protein<\/strong>, <strong>GFP<\/strong>, eller <strong>1GFL<\/strong>.<\/li><li>F\u00f6lj instruktionerna i &#8220;PDBe&#8221; fliken och f\u00f6rs\u00f6k besvara uppgiftsfr\u00e5gorna.<\/li><\/ol>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-4c7c5382-9083-47d1-8a64-9c108e184908\"><h2>PDBe<\/h2>\n<ol class=\"wp-block-list\"><li>\u00d6ppna l\u00e4nken i ett nytt webbl\u00e4sarf\u00f6nster: <a href=\"https:\/\/www.ebi.ac.uk\/pdbe\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.ebi.ac.uk\/pdbe\/<\/a><\/li><li>I s\u00f6krutan skriver du antingen <strong>Green Fluorescent Protein<\/strong>, <strong>GFP<\/strong>, eller <strong>1GFL<\/strong> och trycker p\u00e5 <strong>&#8220;Search&#8221;<\/strong> knappen.<\/li><li>Du har nu f\u00e5tt vidarebefordrat de gr\u00f6n fluorescerande protein (GFP) bidragen i PDBe. Om du klickar p\u00e5 en bild av n\u00e5gon av proteinstrukturerna s\u00e5 \u00f6ppnas en interaktive 3D protein &#8220;viewer&#8221; (alternativt, klicka p\u00e5 &#8220;View in 3D&#8221; och v\u00e4lj d\u00e4refter &#8220;Astex Viewer&#8221;). Var beredd p\u00e5 att du kan bli tillfr\u00e5gad om att ge tillst\u00e5nd till Java i det l\u00e4get. Du ser nu en tredimensionell &#8220;ribbon-cartoon&#8221; struktur av GFP. Du kan anv\u00e4nda mark\u00f6ren till att rotera strukturen. Valda &#8220;features&#8221; (s\u00e5som typ av strukturell representation eller f\u00e4rg) kan modifieras antingen genom att anv\u00e4nda menyn till v\u00e4nster eller genom att h\u00f6ger-klicka p\u00e5 bilden.<\/li><\/ol>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-04943c3b-3a86-4fec-b356-e6cb9688f09d\"><h2>Fr\u00e5gor<\/h2>\n<p>Lek runt med den interaktiva tredimensionella proteinstrukturen och f\u00f6rs\u00f6k besvara n\u00e5gon av f\u00f6ljande fr\u00e5gor:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Vad ser formen p\u00e5 GFP molekylen ut som?<\/li><li>Kan du hitta kromoforen i molekylstrukturen?<\/li><li>Vilken effekt kan mutationer medf\u00f6ra p\u00e5 proteinets funktion?<\/li><\/ul>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-d7a4ed6c-a6b9-4bbf-9b2a-28eb7cacd6a2\"><h2>Aktivitetsnavigering<\/h2>\n<p><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/?lang=sv\">GFP skattjakt<\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/gfp-treasure-hunt-introduction\/?lang=sv\">GFP skattjakt introduktion<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-1-database-search\/?lang=sv\">Steg 1: Databas s\u00f6kning<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-2-comparison-of-nucleotide-sequences\/?lang=sv\">Steg 2: J\u00e4mf\u00f6relse av nukleotidsekvenser<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-3-translation-of-mrnas\/?lang=sv\">Steg 3: Translation av mRNA<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-4-comparison-of-amino-acid-sequences\/?lang=sv\">Steg 4: J\u00e4mf\u00f6relse av aminosyrasekvenser<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/?lang=sv\">Steg 5: GFP struktur<\/a><\/li><\/ul>\n<\/section>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":29379,"parent":28292,"menu_order":1,"template":"","class_list":["post-29378","teachingbase","type-teachingbase","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/29378","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase"}],"about":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/types\/teachingbase"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/28292"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media\/29379"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media?parent=29378"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}