{"id":29370,"date":"2015-10-05T20:05:57","date_gmt":"2015-10-05T18:05:57","guid":{"rendered":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/"},"modified":"2021-06-18T14:46:32","modified_gmt":"2021-06-18T14:46:32","slug":"step-5-structure-of-gfp","status":"publish","type":"teachingbase","link":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/?lang=cs","title":{"rendered":"Krok 5: Struktura proteinu GFP"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-74c95bab-501c-4bc2-bc5c-4bd79038234c\">\u00davod<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-1a6bebfa-8428-433f-9cfc-6506b7154097\">V\u00e1\u0161 \u00fakol<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-4c7c5382-9083-47d1-8a64-9c108e184908\">PDBe<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-04943c3b-3a86-4fec-b356-e6cb9688f09d\">Ot\u00e1zky<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-d7a4ed6c-a6b9-4bbf-9b2a-28eb7cacd6a2\">Navigace aktivity<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-74c95bab-501c-4bc2-bc5c-4bd79038234c\"><h2>\u00davod<\/h2>\n<p>Ve fin\u00e1ln\u00ed \u010d\u00e1sti vyu\u017eijeme datab\u00e1zi Protein Data Bank Europe (PDBe) k zobrazen\u00ed interaktivn\u00edch trojdimenzion\u00e1ln\u00edch struktur proteinu GFP a pr\u00e1ci s t\u011bmito strukturami.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-1a6bebfa-8428-433f-9cfc-6506b7154097\"><h2>V\u00e1\u0161 \u00fakol<\/h2>\n<p><strong>Pokra\u010dujte jak pops\u00e1no n\u00ed\u017ee:<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\"><li>Klikn\u011bte na tabul\u00e1tor &#8220;PDBe&#8221;.<\/li><li>Hledejte&nbsp; <strong>Green Fluorescent Protein<\/strong>, <strong>GFP<\/strong>, nebo <strong>1GFL<\/strong>.<\/li><li>N\u00e1sledujte instrukce v tabul\u00e1toru &#8220;PDBe&#8221; a pokuste se zodpov\u011bd\u011bt ot\u00e1zky.<\/li><\/ol>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-4c7c5382-9083-47d1-8a64-9c108e184908\"><h2>PDBe<\/h2>\n<ol class=\"wp-block-list\"><li>Otev\u0159ete odkaz v nov\u00e9m okn\u011b prohl\u00ed\u017ee\u010de:&nbsp;<a href=\"https:\/\/www.ebi.ac.uk\/pdbe\/\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.ebi.ac.uk\/pdbe\/<\/a><\/li><li>V datab\u00e1zi PDBe zadejte do hledac\u00edho okna bu\u010f&nbsp; <strong>Green Fluorescent Protein<\/strong>, <strong>GFP<\/strong>, nebo <strong>1GFL<\/strong> a zm\u00e1\u010dkn\u011bte tla\u010d\u00edtko &#8220;Search\/hledat&#8221;.<\/li><li>Nyn\u00ed se V\u00e1m zobrazuj\u00ed informace o v\u0161ech GFP proteinech v datab\u00e1zi PDBe. Pokud kliknete na jeden z obr\u00e1zk\u016f proteinov\u00fdch struktur, otev\u0159ete interaktivn\u00ed prohl\u00ed\u017ee\u010d 3D struktur. Otev\u0159en\u00ed vy\u017eaduje nainstalovanou Javu a m\u016f\u017eete b\u00fdt po\u017e\u00e1d\u00e1n\u00ed o povolen\u00ed Javu vyu\u017e\u00edt (alternativn\u011b m\u016f\u017eete kliknout na &#8221; View in 3D\/zobraze v 3D a vyberte &#8220;Astex Viewer&#8221;) . Nyn\u00ed se d\u00edv\u00e1te na 3D strukturu GFP proteinu ve stuhov\u00e9m zobrazen\u00ed. Kursor m\u016f\u017eete pou\u017e\u00edt k rotaci se strukturou. Dal\u0161\u00ed vlastnosti (barvu, typ zobrazen\u00ed) m\u016f\u017eete m\u011bnit d\u00edky menu v lev\u00e9 \u010d\u00e1sti nebo kliknut\u00edm prav\u00fdm tla\u010d\u00edtkem na my\u0161i na obr\u00e1zek.<\/li><\/ol>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-04943c3b-3a86-4fec-b356-e6cb9688f09d\"><h2>Ot\u00e1zky<\/h2>\n<p>Pohrajte se s 3D strukturou GFP proteinu a zodpov\u011bzte n\u00e1sleduj\u00edc\u00ed ot\u00e1zky:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Jak\u00fd tvar m\u00e1 GFP molekula?<\/li><li>Vid\u00edte ve struktu\u0159e chromofor?<\/li><li>Jak\u00fd vliv mohou m\u00edt mutace na funci GFP proteinu?<\/li><\/ul>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-d7a4ed6c-a6b9-4bbf-9b2a-28eb7cacd6a2\"><h2>Navigace aktivity<\/h2>\n<p><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/?lang=cs\">Honba za pokladem &#8211; GFP<\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/gfp-treasure-hunt-introduction\/?lang=cs\">Honba za pokladem &#8211; GFP &#8211; \u00favod<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-1-database-search\/?lang=cs\">Krok 1: Vyhled\u00e1v\u00e1n\u00ed v datab\u00e1zi<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-2-comparison-of-nucleotide-sequences\/?lang=cs\">Krok 2: Porovn\u00e1n\u00ed nukleotidov\u00fdch sekvenc\u00ed<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-3-translation-of-mrnas\/?lang=cs\">Krok 3: P\u0159eklad mRNA<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-4-comparison-of-amino-acid-sequences\/?lang=cs\">Krok 4: Srovn\u00e1n\u00ed aminokyselinov\u00fdch sekvenc\u00ed<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/?lang=cs\">Krok 5: Struktura proteinu GFP<\/a><\/li><\/ul>\n<\/section>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":29371,"parent":28284,"menu_order":1,"template":"","class_list":["post-29370","teachingbase","type-teachingbase","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/29370","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase"}],"about":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/types\/teachingbase"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/28284"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media\/29371"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media?parent=29370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}