{"id":29219,"date":"2015-10-05T20:01:59","date_gmt":"2015-10-05T18:01:59","guid":{"rendered":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-4-comparison-of-amino-acid-sequences\/"},"modified":"2021-06-18T04:08:19","modified_gmt":"2021-06-18T04:08:19","slug":"step-4-comparison-of-amino-acid-sequences","status":"publish","type":"teachingbase","link":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-4-comparison-of-amino-acid-sequences\/?lang=cs","title":{"rendered":"Krok 4: Srovn\u00e1n\u00ed aminokyselinov\u00fdch sekvenc\u00ed"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-06938b10-94be-46c6-be96-2fffd279c159\">\u00davod<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-8a733a57-5480-4758-911e-46ffc6af953b\">V\u00e1\u0161 \u00fakol<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-d194c45f-37fc-4601-8ab3-de9b4974a4a3\">MUSCLE<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-1285552d-049a-4c11-bd79-cfaa40a8f7a7\">Aminokyselinov\u00e9 sekvence<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-7eb8e73c-ae74-4cfe-822f-3c7775beeee2\">Ot\u00e1zky<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-f9fcf67e-c0d4-45cc-b8bc-959d4ee50b10\">Navigace aktivity<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-06938b10-94be-46c6-be96-2fffd279c159\"><h2>\u00davod<\/h2>\n<p>V t\u00e9to \u010d\u00e1sti pou\u017eijeme dv\u011b sekvence z p\u0159edchoz\u00ed \u010d\u00e1sti k ur\u010den\u00ed podobnosti t\u011bchto sekvenc\u00ed za pomoci&nbsp; n\u00e1stroje EBI MUSCLE, kter\u00fd slou\u017e\u00ed ke srovn\u00e1v\u00e1n\u00ed sekvenc\u00ed. Sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed proteinov\u00fdch sekvenc\u00ed je siln\u011bj\u0161\u00ed indic\u00ed\u00ed podobnost\u00ed sekvenc\u00ed ne\u017e sekven\u010dn\u00ed srovn\u00e1n\u00ed zalo\u017een\u00e9 na podobnosti nukleotidov\u00fdch sekvenc\u00ed. saekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed n\u00e1m umo\u017enuje zobrazit inzerce, delece a mutace, ke kter\u00fdm muselo doj\u00edt u obou sekvenc\u00ed na cest\u011b od spole\u010dn\u00e9ho p\u0159edka obou sekvenc\u00ed. Sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed n\u00e1m d\u00e1le umo\u017e\u0148uje tak\u00e9 identifikovat aminokyseliny, kter\u00e9 byly b\u011bhem evoluce dob\u0159e konzervov\u00e1ny.<\/p>\n\n\n\n<p>Na z\u00e1v\u011br t\u00e9to \u010d\u00e1sti budete m\u00edt k dispozici sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed na jeho\u017e z\u00e1klad\u011b budete moci hrub\u011b odhadnout hlavn\u00ed rozd\u00edly mezi ob\u011bma sekvencemi.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-8a733a57-5480-4758-911e-46ffc6af953b\"><h2>V\u00e1\u0161 \u00fakol<\/h2>\n<p><strong>Pokra\u010dujte dle n\u00e1sleduj\u00edc\u00edch instrukc\u00ed:<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\"><li>Zkop\u00edrujte v\u0161echny sekvence z tabul\u00e1toru &#8220;Aminokyselinov\u00e9 sekvence&#8221; (Ctrl+C).<\/li><li>Vlo\u017ete zkop\u00edrovan\u00e9 sekvence do n\u00e1stroje Muscle EBI (Ctrl+V).<\/li><li>N\u00e1sledujte instrukce v tabul\u00e1toru Muscle.<\/li><li>Pod\u00edvejte se n\u00e1 v\u00fdsledek a pokuste se zodpov\u011bd\u011bt p\u0159ilo\u017een\u00e9 ot\u00e1zky.<\/li><\/ol>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-d194c45f-37fc-4601-8ab3-de9b4974a4a3\"><h2>MUSCLE<\/h2>\n<ol class=\"wp-block-list\"><li>Zkop\u00edrujte v\u0161echny sekvence z tabul\u00e1toru &#8220;Aminokyselinov\u00e9 sekvence&#8221; (Ctrl+C).<\/li><li>Vlo\u017ete zkop\u00edrovan\u00e9 sekvence do n\u00e1stroje Muscle EBI (Ctrl+V).<\/li><li>V \u010d\u00e1sti &#8220;STEP 2&#8221; se p\u0159esv\u011bd\u010dte, \u017ee &#8220;Output Format\/ v\u00fdstupn\u00ed form\u00e1t&#8221; je nastaven na Clustal W.<\/li><li>Ozna\u010dte tla\u010d\u00edtko &#8220;Submit\/Ode\u0161lete&#8221; a sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed bude zpracov\u00e1no.<\/li><li>V\u00fdsledky se brzy objev\u00ed.<\/li><li>Pod\u00edvejte se n\u00e1 v\u00fdsledek a pokuste se zodpov\u011bd\u011bt p\u0159ilo\u017een\u00e9 ot\u00e1zky.<\/li><\/ol>\n\n\n<div\n  class=\"vf-embed vf-embed--custom-ratio\"\n\n  style=\"--vf-embed-max-width: 100%;\n    --vf-embed-custom-ratio-x: 640;\n    --vf-embed-custom-ratio-y: 360;\"\n><iframe loading=\"lazy\" width=\"640\" height=\"360\" src=\"https:\/\/www.ebi.ac.uk\/Tools\/msa\/muscle\/\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-1285552d-049a-4c11-bd79-cfaa40a8f7a7\"><h2>Aminokyselinov\u00e9 sekvence<\/h2>\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nProtein sequence 1<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<pre class=\"wp-block-preformatted\"><span style=\"word-break:break-all\"<\/span>&gt;Protein_Sequence1_AVGFP_1\nMSKGEELFTGVVPVLVELDGDVNGQKFSVSGEGEGDATYGKLTLNFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFYKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMGDKPKNGIKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMILLEFVTAARITHGMDELYK<\/pre>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nProtein sequence 2<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<pre class=\"wp-block-preformatted\"><span style=\"word-break:break-all\"<\/span>&gt;Protein_Sequence2_GFPm_1\nMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK<\/pre>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-7eb8e73c-ae74-4cfe-822f-3c7775beeee2\"><h2>Ot\u00e1zky<\/h2>\n<p>Pod\u00edvejte se na v\u00fdsledn\u00e9 sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed a zodpov\u011bzte n\u00e1sleduj\u00edc\u00ed ot\u00e1zky:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Jak pozn\u00e1te kdy jsou na dan\u00e9 pozici stejn\u00e9 aminokyseliny a kdy nejsou sekvence srovn\u00e1ny?<\/li><li>Je&nbsp; tam mnoho koresponduj\u00edc\u00edch aminokyselin? Obsahuje proteinov\u00e9 sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed v\u00edce podobnost\u00ed ne\u017e nukleotidov\u00e9 sekven\u010dn\u00ed srovn\u00e1v\u00e1n\u00ed?<\/li><li>Co zp\u016fsobuje rozd\u00edly mezi nukleotidov\u00fdm a aminokyselinov\u00fdm sekven\u010dn\u00edm srovn\u00e1v\u00e1n\u00edm?<\/li><\/ul>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-f9fcf67e-c0d4-45cc-b8bc-959d4ee50b10\"><h2>Navigace aktivity<\/h2>\n<p><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/?lang=cs\">Honba za pokladem &#8211; GFP<\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/gfp-treasure-hunt-introduction\/?lang=cs\">Honba za pokladem &#8211; GFP &#8211; \u00favod<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-1-database-search\/?lang=cs\">Krok 1: Vyhled\u00e1v\u00e1n\u00ed v datab\u00e1zi<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-2-comparison-of-nucleotide-sequences\/?lang=cs\">Krok 2: Porovn\u00e1n\u00ed nukleotidov\u00fdch sekvenc\u00ed<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-3-translation-of-mrnas\/?lang=cs\">Krok 3: P\u0159eklad mRNA<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-4-comparison-of-amino-acid-sequences\/?lang=cs\">Krok 4: Srovn\u00e1n\u00ed aminokyselinov\u00fdch sekvenc\u00ed<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/?lang=cs\"><strong>Krok 5: Struktura proteinu GFP<\/strong><\/a><\/li><\/ul>\n<\/section>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":28455,"parent":28284,"menu_order":2,"template":"","class_list":["post-29219","teachingbase","type-teachingbase","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/29219","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase"}],"about":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/types\/teachingbase"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/28284"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media\/28455"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media?parent=29219"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}