{"id":29165,"date":"2015-10-05T19:36:46","date_gmt":"2015-10-05T17:36:46","guid":{"rendered":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-2-comparison-of-nucleotide-sequences\/"},"modified":"2021-06-17T17:54:40","modified_gmt":"2021-06-17T17:54:40","slug":"step-2-comparison-of-nucleotide-sequences","status":"publish","type":"teachingbase","link":"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-2-comparison-of-nucleotide-sequences\/?lang=el","title":{"rendered":"\u0392\u03ae\u03bc\u03b1 2: \u03a3\u03cd\u03b3\u03ba\u03c1\u03b9\u03c3\u03b7 \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-da0d437d-5f99-4d34-b6fa-c991f58a5130\">\u03a3\u03a6\u0391\u0399\u03a1\u0399\u039a\u0397 \u0395\u0399\u039a\u039f\u039d\u0391<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-78981bee-9962-4382-92d1-7136ffb3d4e5\">\u0397 \u03b5\u03c1\u03b3\u03b1\u03c3\u03af\u03b1 \u03c3\u03bf\u03c5<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-9c0782e4-2aaa-4390-accc-3ea3048a359c\">\u039b\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03cc MUSCLE<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-d8ee530d-eb3f-41b4-9c68-04224aba4a8f\">\u0391\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 1-4<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-9dfee51b-0a31-47d0-a3de-4ab80431fd4d\">\u0395\u03c1\u03c9\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-1b962e56-7024-4712-9567-b19dfc6cf636\">\u03a0\u03bb\u03bf\u03ae\u03b3\u03b7\u03c3\u03b7 \u03b4\u03c1\u03b1\u03c3\u03c4\u03b7\u03c1\u03b9\u03cc\u03c4\u03b7\u03c4\u03b1\u03c2<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-da0d437d-5f99-4d34-b6fa-c991f58a5130\"><h2>\u03a3\u03a6\u0391\u0399\u03a1\u0399\u039a\u0397 \u0395\u0399\u039a\u039f\u039d\u0391<\/h2>\n<p>\u03a4\u03b1\u03c5\u03c4\u03cc\u03c7\u03c1\u03bf\u03bd\u03b7 \u03c3\u03cd\u03b3\u03ba\u03c1\u03b9\u03c3\u03b7 \u03cc\u03bb\u03c9\u03bd \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd \u03bc\u03c0\u03bf\u03c1\u03b5\u03af \u03bd\u03b1 \u03c0\u03c1\u03b1\u03b3\u03bc\u03b1\u03c4\u03bf\u03c0\u03bf\u03b9\u03b7\u03b8\u03b5\u03af \u03bc\u03ad\u03c3\u03c9 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03ae\u03c3\u03b7\u03c2 \u03c4\u03bf\u03c5 \u03b5\u03b9\u03b4\u03b9\u03ba\u03bf\u03cd \u03bb\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03bf\u03cd \u03c0\u03bf\u03c5 \u03ad\u03c7\u03b5\u03b9 \u03b1\u03bd\u03b1\u03c0\u03c4\u03cd\u03be\u03b5\u03b9 \u03c4\u03bf \u0395\u03c5\u03c1\u03c9\u03c0\u03b1\u03ca\u03ba\u03cc \u0399\u03bd\u03c3\u03c4\u03b9\u03c4\u03bf\u03cd\u03c4\u03bf \u0392\u03b9\u03bf\u03c0\u03bb\u03b7\u03c1\u03bf\u03c6\u03bf\u03c1\u03b9\u03ba\u03ae\u03c2 &#8211; EMBL-EBI \u03ba\u03b1\u03b9 \u03c4\u03bf \u03bf\u03c0\u03bf\u03af\u03bf \u03bf\u03bd\u03bf\u03bc\u03ac\u03b6\u03b5\u03c4\u03b1\u03b9 MUSCLE. (Multiple Sequence Comparison by Log-Expectation). \u03a4\u03bf \u03bb\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03cc MUSCLE \u03c0\u03b1\u03c1\u03ad\u03c7\u03b5\u03b9 <strong>\u03b5\u03c5\u03b8\u03c5\u03b3\u03c1\u03b1\u03bc\u03bc\u03af\u03c3\u03b5\u03b9\u03c2<\/strong><strong>\u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd<\/strong>. \u0391\u03c5\u03c4\u03ad\u03c2 \u03bf\u03b9 \u03b5\u03c5\u03b8\u03c5\u03b3\u03c1\u03b1\u03bc\u03bc\u03af\u03c3\u03b5\u03b9\u03c2 \u03bc\u03c0\u03bf\u03c1\u03bf\u03cd\u03bd \u03bd\u03b1 \u03c7\u03c1\u03b7\u03c3\u03b9\u03bc\u03bf\u03c0\u03bf\u03b9\u03b7\u03b8\u03bf\u03cd\u03bd \u03c4\u03cc\u03c3\u03bf \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03bf\u03c0\u03c4\u03b9\u03ba\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7 \u03cc\u03c3\u03bf \u03ba\u03b1\u03b9 \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03b5\u03c1\u03bc\u03b7\u03bd\u03b5\u03af\u03b1 \u03c4\u03c9\u03bd \u03c3\u03c7\u03ad\u03c3\u03b5\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03be\u03cd \u03c4\u03c9\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd &#8211; \u03ba\u03b1\u03b9 \u03bf\u03c1\u03b9\u03c3\u03bc\u03ad\u03bd\u03b5\u03c2 \u03c6\u03bf\u03c1\u03ad\u03c2 \u03b1\u03ba\u03cc\u03bc\u03b7 \u03ba\u03b1\u03b9 \u03bc\u03b5\u03c4\u03b1\u03be\u03cd \u03c4\u03c9\u03bd \u03b5\u03b9\u03b4\u03ce\u03bd \u03bf\u03c1\u03b3\u03b1\u03bd\u03b9\u03c3\u03bc\u03ce\u03bd \u03c3\u03c4\u03b1 \u03bf\u03c0\u03bf\u03af\u03b1 \u03b1\u03bd\u03ae\u03ba\u03bf\u03c5\u03bd.<\/p>\n\n\n\n<p>\u039c\u03b5\u03c4\u03ac \u03c4\u03b7\u03bd \u03bf\u03bb\u03bf\u03ba\u03bb\u03ae\u03c1\u03c9\u03c3\u03b7 \u03b1\u03c5\u03c4\u03ae\u03c2 \u03c4\u03b7\u03c2 \u03b4\u03c1\u03b1\u03c3\u03c4\u03b7\u03c1\u03b9\u03cc\u03c4\u03b7\u03c4\u03b1\u03c2 \u03b8\u03b1 \u03ad\u03c7\u03b5\u03b9\u03c2 \u03c0\u03b1\u03c1\u03ac\u03be\u03b5\u03b9 \u03bc\u03b9\u03b1 \u03c0\u03bf\u03bb\u03bb\u03b1\u03c0\u03bb\u03ae \u03b5\u03c5\u03b8\u03c5\u03b3\u03c1\u03ac\u03bc\u03bc\u03b9\u03c3\u03b7 \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd (\u03c3\u03b5 \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03cc \u03b5\u03c0\u03af\u03c0\u03b5\u03b4\u03bf) \u03ba\u03b1\u03b9 \u03b8\u03b1 \u03b5\u03af\u03c3\u03b1\u03b9 \u03c3\u03b5 \u03b8\u03ad\u03c3\u03b7 \u03bd\u03b1 \u03b5\u03ba\u03c4\u03b9\u03bc\u03ae\u03c3\u03b5\u03b9\u03c2 \u03c0\u03bf\u03b9\u03b5\u03c2 \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ad\u03c2 \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 \u03b5\u03af\u03bd\u03b1\u03b9 \u03c0\u03b9\u03bf \u03cc\u03bc\u03bf\u03b9\u03b5\u03c2 \u03bc\u03b5\u03c4\u03b1\u03be\u03cd \u03c4\u03bf\u03c5\u03c2. \u039c\u03c0\u03bf\u03c1\u03b5\u03af\u03c2 \u03bd\u03b1 \u03b4\u03b5\u03b9\u03c2 \u03c4\u03b9\u03c2 \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ad\u03c2 \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 \u03c0\u03bf\u03c5 \u03b5\u03b9\u03c3\u03ae\u03b3\u03b1\u03b3\u03b5\u03c2 (input) \u03c3\u03c4\u03bf \u03c0\u03b5\u03b4\u03af\u03bf &#8220;\u0391\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2&#8221;.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-78981bee-9962-4382-92d1-7136ffb3d4e5\"><h2>\u0397 \u03b5\u03c1\u03b3\u03b1\u03c3\u03af\u03b1 \u03c3\u03bf\u03c5<\/h2>\n<ol class=\"wp-block-list\"><li>\u039a\u03ac\u03bd\u03b5 \u03b1\u03bd\u03c4\u03b9\u03b3\u03c1\u03b1\u03c6\u03ae \u03cc\u03bb\u03c9\u03bd \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd \u03c0\u03bf\u03c5 \u03b2\u03c1\u03af\u03c3\u03ba\u03bf\u03bd\u03c4\u03b1\u03b9 \u03c3\u03c4\u03bf \u03c0\u03b5\u03b4\u03af\u03bf &#8220;\u0391\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2&#8221; (\u03b3\u03b9\u03b1 \u03c0\u03b1\u03c1\u03ac\u03b4\u03b5\u03b9\u03b3\u03bc\u03b1 \u03b5\u03c0\u03af\u03bb\u03b5\u03be\u03ad \u03c4\u03b5\u03c2 \u03cc\u03bb\u03b5\u03c2 \u03bc\u03b1\u03b6\u03af \u03ba\u03b1\u03b9 \u03c3\u03c4\u03b7 \u03c3\u03c5\u03bd\u03ad\u03c7\u03b5\u03b9\u03b1 \u03b1\u03bd\u03c4\u03af\u03b3\u03c1\u03b1\u03c8\u03ad \u03c4\u03b5\u03c2 \u03bc\u03b5 \u03c4\u03b1 \u03c0\u03bb\u03ae\u03ba\u03c4\u03c1\u03b1 Ctrl. + C).<\/li><li>\u039a\u03ac\u03bd\u03b5 \u03b5\u03c0\u03b9\u03ba\u03cc\u03bb\u03bb\u03b7\u03c3\u03b7 \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd \u03c3\u03c4\u03bf \u03c0\u03b5\u03b4\u03af\u03bf \u03b1\u03bd\u03b1\u03b6\u03ae\u03c4\u03b7\u03c3\u03b7\u03c2 \u03c4\u03bf\u03c5 \u03bb\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03bf\u03cd MUSCLE (\u03bc\u03c0\u03bf\u03c1\u03b5\u03af\u03c2 \u03bd\u03b1 \u03c4\u03bf \u03ba\u03ac\u03bd\u03b5\u03b9\u03c2 \u03b1\u03c5\u03c4\u03cc \u03bc\u03b5 \u03c4\u03b1 \u03c0\u03bb\u03ae\u03ba\u03c4\u03c1\u03b1 Ctrl. + V).<\/li><li>\u0391\u03ba\u03bf\u03bb\u03bf\u03cd\u03b8\u03b7\u03c3\u03b5 \u03c4\u03b9\u03c2 \u03bf\u03b4\u03b7\u03b3\u03af\u03b5\u03c2 \u03c4\u03bf\u03c5 \u03bb\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03bf\u03cd &#8220;MUSCLE&#8221; \u03b3\u03b9\u03b1 \u03bd\u03b1 \u03b5\u03c5\u03b8\u03c5\u03b3\u03c1\u03b1\u03bc\u03bc\u03af\u03c3\u03b5\u03b9\u03c2 \u03c4\u03b9\u03c2 \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 \u03c3\u03bf\u03c5.<\/li><li>\u0394\u03b5\u03c2 \u03c4\u03bf \u03b1\u03c0\u03bf\u03c4\u03ad\u03bb\u03b5\u03c3\u03bc\u03b1 \u03c4\u03b7\u03c2 \u03b5\u03c5\u03b8\u03c5\u03b3\u03c1\u03ac\u03bc\u03bc\u03b9\u03c3\u03b7\u03c2 \u03ba\u03b1\u03b9 \u03c0\u03c1\u03bf\u03c3\u03c0\u03ac\u03b8\u03b7\u03c3\u03b5 \u03bd\u03b1 \u03b1\u03c0\u03b1\u03bd\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2 \u03c4\u03b9\u03c2 \u03b5\u03c1\u03c9\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2 \u03c3\u03c4\u03bf \u03b1\u03bd\u03c4\u03af\u03c3\u03c4\u03bf\u03b9\u03c7\u03bf \u03c0\u03b5\u03b4\u03af\u03bf.<\/li><\/ol>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-9c0782e4-2aaa-4390-accc-3ea3048a359c\"><h2>\u039b\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03cc MUSCLE<\/h2>\n<ol class=\"wp-block-list\"><li>\u039a\u03ac\u03bd\u03b5 \u03b1\u03bd\u03c4\u03b9\u03b3\u03c1\u03b1\u03c6\u03ae \u03cc\u03bb\u03c9\u03bd \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd \u03c0\u03bf\u03c5 \u03b2\u03c1\u03af\u03c3\u03ba\u03bf\u03bd\u03c4\u03b1\u03b9 \u03c3\u03c4\u03bf \u03c0\u03b5\u03b4\u03af\u03bf &#8220;\u0391\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2&#8221; (\u03b3\u03b9\u03b1 \u03c0\u03b1\u03c1\u03ac\u03b4\u03b5\u03b9\u03b3\u03bc\u03b1 \u03b5\u03c0\u03af\u03bb\u03b5\u03be\u03ad \u03c4\u03b5\u03c2 \u03cc\u03bb\u03b5\u03c2 \u03bc\u03b1\u03b6\u03af \u03ba\u03b1\u03b9 \u03c3\u03c4\u03b7 \u03c3\u03c5\u03bd\u03ad\u03c7\u03b5\u03b9\u03b1 \u03b1\u03bd\u03c4\u03af\u03b3\u03c1\u03b1\u03c8\u03ad \u03c4\u03b5\u03c2 \u03bc\u03b5 \u03c4\u03b1 \u03c0\u03bb\u03ae\u03ba\u03c4\u03c1\u03b1 Ctrl. + C).<\/li><li>\u039a\u03ac\u03bd\u03b5 \u03b5\u03c0\u03b9\u03ba\u03cc\u03bb\u03bb\u03b7\u03c3\u03b7 \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd \u03c3\u03c4\u03bf \u03c0\u03b5\u03b4\u03af\u03bf \u03b1\u03bd\u03b1\u03b6\u03ae\u03c4\u03b7\u03c3\u03b7\u03c2 \u03c4\u03bf\u03c5 \u03bb\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03bf\u03cd MUSCLE (\u03bc\u03c0\u03bf\u03c1\u03b5\u03af\u03c2 \u03bd\u03b1 \u03c4\u03bf \u03ba\u03ac\u03bd\u03b5\u03b9\u03c2 \u03b1\u03c5\u03c4\u03cc \u03bc\u03b5 \u03c4\u03b1 \u03c0\u03bb\u03ae\u03ba\u03c4\u03c1\u03b1 Ctrl. + V).<\/li><li>\u03a3\u03c4\u03bf &#8220;\u0392\u03ae\u03bc\u03b1 2&#8221; \u03ad\u03bb\u03b5\u03b3\u03be\u03b5 \u03b1\u03bd \u03c4\u03bf &#8220;<strong>Output Format<\/strong>&#8221; \u03b5\u03af\u03bd\u03b1\u03b9 \u03c1\u03c5\u03b8\u03bc\u03b9\u03c3\u03bc\u03ad\u03bd\u03bf \u03c3\u03c4\u03bf<strong>&#8220;ClustalW&#8221;<\/strong>.<\/li><li>\u039a\u03ac\u03bd\u03b5 \u03ba\u03bb\u03b9\u03ba \u03c3\u03c4\u03bf \u03ba\u03bf\u03c5\u03bc\u03c0\u03af <strong>&#8220;Submit<\/strong>&#8221; \u03ba\u03b1\u03b9 \u03c4\u03bf \u03bb\u03bf\u03b3\u03b9\u03c3\u03bc\u03b9\u03ba\u03cc \u03b8\u03b1 \u03b5\u03c0\u03b5\u03be\u03b5\u03c1\u03b3\u03b1\u03c3\u03c4\u03b5\u03af \u03c4\u03b9\u03c2 \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 \u03c0\u03bf\u03c5 \u03b5\u03b9\u03c3\u03ae\u03b3\u03b1\u03b3\u03b5\u03c2.<\/li><li>\u0398\u03b1 \u03b4\u03b5\u03b9\u03c2 \u03c4\u03b1 \u03b1\u03c0\u03bf\u03c4\u03b5\u03bb\u03ad\u03c3\u03bc\u03b1\u03c4\u03b1 \u03c4\u03b7\u03c2 \u03b5\u03c0\u03b5\u03be\u03b5\u03c1\u03b3\u03b1\u03c3\u03af\u03b1\u03c2 \u03bc\u03b5\u03c4\u03ac \u03b1\u03c0\u03cc \u03bc\u03b5\u03c1\u03b9\u03ba\u03ac \u03b4\u03b5\u03c5\u03c4\u03b5\u03c1\u03cc\u03bb\u03b5\u03c0\u03c4\u03b1. \u03a0\u03c1\u03bf\u03c3\u03c0\u03ac\u03b8\u03b7\u03c3\u03b5 \u03bd\u03b1 \u03b1\u03c0\u03b1\u03bd\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2 \u03c4\u03b9\u03c2 \u03b5\u03c1\u03c9\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2 \u03c0\u03bf\u03c5 \u03c3\u03c7\u03b5\u03c4\u03af\u03b6\u03bf\u03bd\u03c4\u03b1\u03b9 \u03bc\u03b5 \u03c4\u03b1 \u03b1\u03c0\u03bf\u03c4\u03b5\u03bb\u03ad\u03c3\u03bc\u03b1\u03c4\u03ac \u03c3\u03bf\u03c5 \u03c3\u03c4\u03bf \u03b1\u03bd\u03c4\u03af\u03c3\u03c4\u03bf\u03b9\u03c7\u03bf \u03c0\u03b5\u03b4\u03af\u03bf.<\/li><\/ol>\n\n\n<div\n  class=\"vf-embed vf-embed--custom-ratio\"\n\n  style=\"--vf-embed-max-width: 100%;\n    --vf-embed-custom-ratio-x: 640;\n    --vf-embed-custom-ratio-y: 360;\"\n><iframe loading=\"lazy\" width=\"640\" height=\"360\" src=\"https:\/\/www.ebi.ac.uk\/Tools\/msa\/muscle\/\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-d8ee530d-eb3f-41b4-9c68-04224aba4a8f\"><h2>\u0391\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 1-4<\/h2>\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nA 1<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<pre class=\"wp-block-preformatted\"><span style=\"word-break:break-all\"<\/span>&gt;Sequence1_AVGFP\nATGAGTAAAGGAGAAGAACTTTTCACTGGAGTGGTCCCAGTTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAATTTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTCTCAAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGGAGACAAACCAAAGAATGGCATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTAGGATTACACATGGCATGGATGAACTATACAAA<\/pre>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nA 2<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<pre class=\"wp-block-preformatted\"> <span style=\"word-break:break-all\"<\/span>&gt;Sequence2_GFPm\nATGTCTAAAGGTGAAGAATTATTCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTCTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTTGTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTGTTCAATGTTTTGCTAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCATGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGACCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTATTGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCACAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAATTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTATCCAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGGTATTACCCATGGTATGGATGAATTGTACAAATAACTGCAG<\/pre>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nA 3<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<pre class=\"wp-block-preformatted\"><span style=\"word-break:break-all\"<\/span>&gt;Sequence3_YFP\nAATATTTTTATTAATTCATTAGAAAAATGAGAGGAAGGATTATTATGTTTAAAGGTATAGTAGAAGGTATAGGAATCATTGAAAAAATTGATATATATACTGACCTAGATAAGTATGCAATTCGATTTCCTGAAAATATGTTGAATGGAATTAAAAAGGAGTCGTCAATAATGTTTAACGGATGCTTCTTAACGGTAACTAGCGTGAATTCAAACATTGTCTGGTTTGATATATTTGAAAAAGAAGCACGTAAGCTTGATACTTTTCGGGAATATAAGGTAGGTGACCGAGTAAATTTAGGAACATTCCCAAAATTTGGCGCTGCATCTGGTGGGCATATATTATCAGCAAGGATTTCATGTGTAGCAAGTATTATTGAAATAATAGAAAATGAGGATTATCAACAAATGTGGATTCAAATTCCTGAAAATTTTACAGAGTTTCTTATTGATAAAGACTATATTGCTGTGGATGGTATTAGCTTAACTATTGACACTATAAAAAACAACCAATTTTTCATTAGTTTACCCTTAAAAATAGCACAAAATACAAATATGAAATGGCGAAAAAAAGGTGATAAGGTAAATGTTGAGTTATCAAACAAAATTAATGCTAACCAGTGTTGGTAATTTACTGAGGATAGTAAAAATGAACTGTTTAAAATAATATTTAAATTTTTATTTATAATACAGAGTCAGTTGTTGTAAATAGTCTGAGTGGTAAATAAGTTCTACCATTAATTAAATATTATCCATATTAAATAAAGGATCT<\/pre>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nA 4<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<pre class=\"wp-block-preformatted\"><span style=\"word-break:break-all\"<\/span>&gt;Sequence4_RFP\nAGTTTCAGCCAGTGACAGGGTGAGCTGCCAGGTATTCTAACAAGATGAGTTGTTCCAAGAATGTGATCAAGGAGTTCATGAGGTTCAAGGTTCGTATGGAAGGAACGGTCAATGGGCACGAGTTTGAAATAAAAGGCGAAGGTGAAGGGAGGCCTTACGAAGGTCACTGTTCCGTAAAGCTTATGGTAACCAAGGGTGGACCTTTGCCATTTGCTTTTGATATTTTGTCACCACAATTTCAGTATGGAAGCAAGGTATATGTCAAACACCCTGCCGACATACCAGACTATAAAAAGCTGTCATTTCCTGAGGGATTTAAATGGGAAAGGGTCATGAACTTTGAAGACGGTGGCGTGGTTACTGTATCCCAAGATTCCAGTTTGAAAGACGGCTGTTTCATCTACGAGGTCAAGTTCATTGGGGTGAACTTTCCTTCTGATGGACCTGTTATGCAGAGGAGGACACGGGGCTGGGAAGCCAGCTCTGAGCGTTTGTATCCTCGTGATGGGGTGCTGAAAGGAGACATCCATATGGCTCTGAGGCTGGAAGGAGGCGGCCATTACCTCGTTGAATTCAAAAGTATTTACATGGTAAAGAAGCCTTCAGTGCAGTTGCCAGGCTACTATTATGTTGACTCCAAACTGGATATGACGAGCCACAACGAAGATTACACAGTCGTTGAGCAGTATGAAAAAACCCAGGGACGCCACCATCCGTTCATTAAGCCTCTGCAGTGAACTCGGCTCAGTCATGGATTAGCGGTAATGGCCACAAAAGGCACGATGATCGTTTTTTAGGAATGCAGCCAAAAATTGAAGGTTATGACAGTAGAAATACAAGCAACAGGCTTTGCTTATTAAACATGTAATTGAAAAC<\/pre>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-9dfee51b-0a31-47d0-a3de-4ab80431fd4d\"><h2>\u0395\u03c1\u03c9\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2<\/h2>\n<p>\u0394\u03b5\u03c2 \u03c4\u03b7\u03bd \u03b5\u03c5\u03b8\u03c5\u03b3\u03c1\u03ac\u03bc\u03bc\u03b9\u03c3\u03b7 \u03c4\u03c9\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd \u03c3\u03bf\u03c5 \u03ba\u03b1\u03b9 \u03c0\u03c1\u03bf\u03c3\u03c0\u03ac\u03b8\u03b7\u03c3\u03b5 \u03bd\u03b1 \u03b1\u03c0\u03b1\u03bd\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2 \u03c3\u03c4\u03b9\u03c2 \u03b5\u03c0\u03cc\u03bc\u03b5\u03bd\u03b5\u03c2 \u03b5\u03c1\u03c9\u03c4\u03ae\u03c3\u03b5\u03b9\u03c2:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u03a0\u03c9\u03c2 \u03bc\u03c0\u03bf\u03c1\u03b5\u03af\u03c2 \u03bd\u03b1 \u03b3\u03bd\u03c9\u03c1\u03af\u03b6\u03b5\u03b9\u03c2 \u03b1\u03bd \u03c4\u03b1 \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03af\u03b4\u03b9\u03b1 \u03ba\u03ac\u03b8\u03b5 \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b1\u03c2 \u03b5\u03af\u03bd\u03b1\u03b9 \u03c4\u03b1 \u03af\u03b4\u03b9\u03b1 \u03ae \u03cc\u03c7\u03b9 \u03bc\u03b5 \u03b1\u03c5\u03c4\u03ac \u03c4\u03c9\u03bd \u03c5\u03c0\u03bf\u03bb\u03bf\u03af\u03c0\u03c9\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd;<\/li><li>\u03a0\u03b9\u03c3\u03c4\u03b5\u03cd\u03b5\u03b9\u03c2 \u03cc\u03c4\u03b9 \u03c5\u03c0\u03ac\u03c1\u03c7\u03b5\u03b9 \u03bc\u03b5\u03b3\u03ac\u03bb\u03bf\u03c2 \u03b1\u03c1\u03b9\u03b8\u03bc\u03cc\u03c2 \u03cc\u03bc\u03bf\u03b9\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03af\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03be\u03cd \u03c4\u03c9\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd; \u03a4\u03bf \u03c0\u03b5\u03c1\u03af\u03bc\u03b5\u03bd\u03b5\u03c2 \u03b1\u03c5\u03c4\u03cc; \u0395\u03be\u03ae\u03b3\u03b7\u03c3\u03b5.<\/li><li>\u03a0\u03bf\u03b9\u03b5\u03c2 \u03b4\u03cd\u03bf \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03af\u03b5\u03c2 \u03ba\u03c1\u03af\u03bd\u03b5\u03b9\u03c2 \u03cc\u03c4\u03b9 \u03b5\u03af\u03bd\u03b1\u03b9 \u03c0\u03b5\u03c1\u03b9\u03c3\u03c3\u03cc\u03c4\u03b5\u03c1\u03bf \u03cc\u03bc\u03bf\u03b9\u03b5\u03c2 \u03bc\u03b5\u03c4\u03b1\u03be\u03cd \u03c4\u03bf\u03c5\u03c2;\u0393\u03b9\u03b1\u03c4\u03af;<\/li><\/ul>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-1b962e56-7024-4712-9567-b19dfc6cf636\"><h2>\u03a0\u03bb\u03bf\u03ae\u03b3\u03b7\u03c3\u03b7 \u03b4\u03c1\u03b1\u03c3\u03c4\u03b7\u03c1\u03b9\u03cc\u03c4\u03b7\u03c4\u03b1\u03c2<\/h2>\n<p><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/?lang=el\">\u039a\u03c5\u03bd\u03ae\u03b3\u03b9 \u03b8\u03b7\u03c3\u03b1\u03c5\u03c1\u03bf\u03cd \u03c4\u03b7\u03c2 \u03c0\u03c1\u03ac\u03c3\u03b9\u03bd\u03b7\u03c2 \u03c6\u03b8\u03bf\u03c1\u03af\u03b6\u03bf\u03c5\u03c3\u03b1\u03c2 \u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b7\u03c2 &#8211; GFP<\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/gfp-treasure-hunt-introduction\/?lang=el\">\u0395\u03b9\u03c3\u03b1\u03b3\u03c9\u03b3\u03ae \u03c3\u03c4\u03bf \u03ba\u03c5\u03bd\u03ae\u03b3\u03b9 \u03b8\u03b7\u03c3\u03b1\u03c5\u03c1\u03bf\u03cd \u03c4\u03b7\u03c2 \u03c0\u03c1\u03ac\u03c3\u03b9\u03bd\u03b7\u03c2 \u03c6\u03b8\u03bf\u03c1\u03af\u03b6\u03bf\u03c5\u03c3\u03b1\u03c2 \u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b7\u03c2 &#8211; GFP<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-1-database-search\/?lang=el\">\u0392\u03ae\u03bc\u03b1 1: \u0391\u03bd\u03b1\u03b6\u03ae\u03c4\u03b7\u03c3\u03b7 \u03c3\u03c4\u03b7 \u03b2\u03ac\u03c3\u03b7 \u03b4\u03b5\u03b4\u03bf\u03bc\u03ad\u03bd\u03c9\u03bd \u201c\u0395\u039d\u0391\u201d<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-2-comparison-of-nucleotide-sequences\/?lang=el\">\u0392\u03ae\u03bc\u03b1 2: \u03a3\u03cd\u03b3\u03ba\u03c1\u03b9\u03c3\u03b7 \u03c4\u03c9\u03bd \u03bd\u03bf\u03c5\u03ba\u03bb\u03b5\u03bf\u03c4\u03b9\u03b4\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-3-translation-of-mrnas\/?lang=el\"><strong>\u0392\u03ae\u03bc\u03b1 3: \u039c\u03b5\u03c4\u03ac\u03c6\u03c1\u03b1\u03c3\u03b7 \u03c4\u03c9\u03bd mRNAs<\/strong><\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-4-comparison-of-amino-acid-sequences\/?lang=el\">\u0392\u03ae\u03bc\u03b1 4: \u03a3\u03cd\u03b3\u03ba\u03c1\u03b9\u03c3\u03b7 \u03b1\u03bc\u03b9\u03bd\u03bf\u03be\u03b9\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03bf\u03c5\u03c7\u03b9\u03ce\u03bd<\/a><\/li><li><a href=\"https:\/\/www.embl.org\/ells\/teachingbase\/gfp-treasure-hunt\/step-5-structure-of-gfp\/?lang=el\">\u0392\u03ae\u03bc\u03b1 5: \u0394\u03bf\u03bc\u03ae \u03c4\u03b7\u03c2 \u03c0\u03c1\u03ac\u03c3\u03b9\u03bd\u03b7\u03c2 \u03c6\u03b8\u03bf\u03c1\u03af\u03b6\u03bf\u03c5\u03c3\u03b1\u03c2 \u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b7\u03c2 &#8211; GFP<\/a><\/li><\/ul>\n<\/section>\n<\/div><\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":29166,"parent":28288,"menu_order":4,"template":"","class_list":["post-29165","teachingbase","type-teachingbase","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/29165","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase"}],"about":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/types\/teachingbase"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/28288"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media\/29166"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media?parent=29165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}