{"id":24849,"date":"2012-04-10T17:10:49","date_gmt":"2012-04-10T15:10:49","guid":{"rendered":"http:\/\/emblog.embl.de\/ells\/?page_id=1748"},"modified":"2021-06-18T14:06:31","modified_gmt":"2021-06-18T14:06:31","slug":"dna-isolation","status":"publish","type":"teachingbase","link":"https:\/\/www.embl.org\/ells\/teachingbase\/dna-isolation\/","title":{"rendered":"DNA isolation from bananas \u2013 kitchen experiment"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-9c155076-1252-49c8-a895-4ba6e9fa8181\">Overview<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-502c52b8-80bc-4338-8b99-9c762593b3c6\">Materials<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-73f30e7e-23ce-42b0-8185-7ce2724f17f3\">Protocol<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-9c155076-1252-49c8-a895-4ba6e9fa8181\"><h2>Overview<\/h2>\n<h3 class=\"wp-block-heading\"><strong>DNA isolation from bananas \u2013 kitchen experiment<\/strong><\/h3>\n\n\n\n<p>Deoxyribonucleic acid (DNA) is the carrier of heritable information in all living organisms. In humans, DNA can be found inside almost every cell of the body. As in bananas, DNA is located within the cell in an even smaller compartment, the cell nucleus. With a few simple household items, you can isolate the DNA of bananas in your kitchen. In doing so, you follow the same principles as the biological researchers when they isolate DNA in the laboratory.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-502c52b8-80bc-4338-8b99-9c762593b3c6\"><h2>Materials<\/h2>\n<p>To run the experiment, you will need:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>3 cm piece of a ripe banana<\/li><li>8 g table salt (NaCl)<\/li><li>10 ml washing-up liquid<\/li><li>16 ml ice-cold 95-100% alcohol (e.g. burnspiritus)<\/li><li>600 ml water<\/li><li>1 fork<\/li><li>3 table spoons<\/li><li>4 tea spoons<\/li><li>1 small plate<\/li><li>2 small bowls (min. 200 ml volume)<\/li><li>2 glasses<\/li><li>small, narrow glass (ca. 30 ml volume, e.g. shot glass)<\/li><li>small glass or plastic container, optional (ca. 10 ml volume)<\/li><li>1 funnel1 piece of kitchen roll<\/li><li>1 wooden toothpick or wooden stick<\/li><li>1 kitchen scale<\/li><li>1 measuring cup<\/li><\/ul>\n\n\n\n<p><\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-73f30e7e-23ce-42b0-8185-7ce2724f17f3\"><h2>Protocol<\/h2>\n<p>View and download protocol: <\/p>\n\n\n\n<div class=\"wp-block-file\"><a  href=\"https:\/\/www.embl.org\/ells\/wp-content\/uploads\/2021\/05\/Banana-DNA-isolation_TeachingBASE_EN.pdf\" class=\"wp-block-file__button vf-button vf-button--primary vf-button--pill vf-button--s\" download style=\"margin:0 0 0 .75em;\">Download<\/a><\/div>\n<\/section>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Simple kitchen-proof experiment about isolating DNA from bananas<\/p>\n","protected":false},"featured_media":25888,"parent":0,"menu_order":13,"template":"","class_list":["post-24849","teachingbase","type-teachingbase","status-publish","has-post-thumbnail","hentry","age-group-05-10","age-group-10-12","age-group-12-14","topic-area-genome-biology","topic-area-methods-in-molecular-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase\/24849","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/teachingbase"}],"about":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/types\/teachingbase"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media\/25888"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/ells\/wp-json\/wp\/v2\/media?parent=24849"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}