{"id":26848,"date":"2021-05-22T16:21:06","date_gmt":"2021-05-22T16:21:06","guid":{"rendered":"https:\/\/www.embl.org\/ells\/?page_id=26848"},"modified":"2021-06-17T16:32:00","modified_gmt":"2021-06-17T16:32:00","slug":"quarry-life-award-2016","status":"publish","type":"page","link":"https:\/\/www.embl.org\/ells\/projects\/quarry-life-award-2016\/","title":{"rendered":"Quarry Life Award 2016"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Quarry Life Award is an international scientific and education contest by HeidelbergCement and its subsidiaries which aims at promoting the awareness of the biodiversity value of quarrying sites. EMBL\u2019s European Learning Laboratory for the Life Sciences (ELLS) in collaboration with Marie-Baum-Schule Heidelberg took part in the contest in 2016. Our project \u201cQuarry biodiversity in focus \u2013 exploring the barcode of life\u201d won the <a rel=\"noreferrer noopener\" href=\"https:\/\/www.heidelbergcement.de\/de\/qla\/preistraeger2016\" target=\"_blank\">Third Prize of the German Quarry Life Award 2016<\/a>.<\/p>\n\n\n\n<p>You can read more about the project and watch the official project video in German language on the <a rel=\"noreferrer noopener\" href=\"https:\/\/www.heidelbergcement.de\/de\/artenvielfalt\/barcode-des-lebens\" target=\"_blank\">Quarry Life Award website<\/a>.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"wp-block-gallery columns-1 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\"><ul class=\"blocks-gallery-grid\"><li class=\"blocks-gallery-item\"><figure><img decoding=\"async\" src=\"https:\/\/www.embl.org\/ells\/wp-content\/uploads\/2021\/05\/Sample3_1_IMG_2166S-e1621703528924-300x180.png\" alt=\"\" data-id=\"1381\" data-full-url=\"https:\/\/www.embl.org\/ells\/wp-content\/uploads\/2021\/05\/Sample3_1_IMG_2166S-e1621703528924.png\" data-link=\"https:\/\/www.embl.org\/ells\/sample3_1_img_2166s\/\" class=\"wp-image-1381\"\/><\/figure><\/li><\/ul><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-c9305a43-ec25-4a05-869b-3ccb0d85644e\">Project description<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-97a5faae-6f89-4309-bb09-80a9ec905af3\">Students\u2019s activity reports<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-f70fdb69-09ac-4c31-8fd8-19d39b34acaa\">Project key dates<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-c9305a43-ec25-4a05-869b-3ccb0d85644e\"><h2>Project description<\/h2>\n<p>Our innovative project \u201cQuarry biodiversity in focus \u2013 exploring the barcode of life\u201d harnessed the power of DNA technology as analysis and science education tool to assess, with molecular resolution, the biodiversity in HeidelbergCement\u2019s restored quarry in Nussloch, Germany.<\/p>\n\n\n\n<p>To address the project\u2019s research question, we established a unique collaboration between science educators of the European Learning Laboratory for the Life Sciences (ELLS) at the European Molecular Biology Laboratory (EMBL) and senior secondary school students from four different classes and their teacher at the Marie-Baum-Schule Heidelberg (MBS).<\/p>\n\n\n\n<p>In order to assess the biodiversity of plants in different habitats within the restored quarry Nussloch, we employed a well-consolidated scientific technology \u2013 DNA barcoding. Using this technology, we analysed and compared plants based on their molecular DNA signature. More specifically, our project employed DNA barcoding to assess and compare plant biodiversity in two particular ecological niches in direct proximity: a heavy metal heap on a historical mining site and a rough pasture. The combination of field work with DNA barcoding and the use of modern biological databases providing open-access to DNA sequence data allowed us to increase our understanding of plant biodiversity in these specific ecological niches in the quarry. By successively carrying out all steps of the DNA barcoding process \u2013 from plant collection and molecular biology in the laboratory to bioinformatics analysis \u2013 the students\u2019 interest in biodiversity-related STEM topics was boosted and raised their awareness of the importance of biodiversity management, particularly in restored mining sites.<\/p>\n\n\n\n<p>Our aim has been to contribute knowledge and data to relevant biological questions relating to quarry biodiversity. The DNA sequence data we generated through our project work has been combined with geolocation data and allows tracking of the plant species identified with smartphone GPS accuracy. For the first time \u2013 and as an outcome of our student research project \u2013 a compilation of newly generated data sets from biological and geographical sources has been used to establish an online repository cataloguing plant biodiversity in the analysed quarry habitats. This open-access online resource could be extended in the future and might be used for HeidelbergCement\u2019s targeted conservation and restoration approach.<\/p>\n\n\n\n<p>The outcomes of this project are directly fostering the fascination for the uniqueness of quarry habitats. Based on the principle \u201cwe can only protect what we know\u201d, our project has further raised public awareness of the importance of biodiversity conservation to safeguard the rich ecosystems in restored quarries.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-97a5faae-6f89-4309-bb09-80a9ec905af3\"><h2>Students\u2019s activity reports<\/h2>\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nIntroductory session at the Marie-Baum-Schule Heidelberg<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>Science educators of the European Learning Laboratory for the Life Sciences (ELLS) organised an informative meeting for the group of MBS high-school students to familiarise themselves with the principle of DNA barcoding and to discuss a detailed project plan with them. Supervised by their biotechnology teacher Frank Luft the students will engage in this unique collaboration with the ELLS team and biodiversity experts from HeidelbergCement to assess plant biodiversity in the company\u2019s quarry in Nussloch. The question they hope to answer is related to plants growing on overburden dumps with high levels of heavy metals as they surface in this quarry: Which plant species are able to grow in this environment posing particular challenges to the vegetation? It will be interesting to find out if during evolution distinct (sub)species have evolved that are able to tolerate larger amounts of heavy metals in the substrate they are growing on. Apart from gaining a detailed understanding of plant biodiversity in specific ecological niches of the quarry the team will try to answer additional questions. The whole team is looking forward to collaborate on this exciting project during the coming months and to inform regularly about their progress via the Quarry Life Award website. Stay tuned!<\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nExcursion to the Nussloch quarry \u2013 green light for plant collection<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>On 9 May 2016 our QLA Project began with a field trip to the Nussloch quarry, where we collected as many different plant samples as possible. After school it finally started, we were picked up by the EMBL bus and driven to the quarry in Nussloch. After a short refreshment we received an informative biology lecture on the quarry by Tina G\u00f6lzer, an ecologist working at Heidelberg Cement. We then went in teams to work on collecting as many plant species as possible, to document their specific features and to take pictures of them. First we searched on the heavy metal heap and then on the rough pasture. If possible, we tried to find the same plant species in both areas, in order to afterwards analyze them in the EMBL lab. Exhausted after this successful day in the quarry \u2013 and full of anticipation for the coming lab day \u2013 we made our way home.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Exkursion zum Steinbruch Nussloch \u2013 alles im gr\u00fcnen Bereich!<\/em><\/h4>\n\n\n\n<p><em>Am 9. Mai 2016 startete die praktische Phase unseres Quarry Life Award Projektes mit einer Exkursion in den Steinbruch Nussloch, um dort m\u00f6glichst viele verschiedene Pflanzenproben zu sammeln. Nach der Schule ging es endlich los und wir wurden mit dem EMBL-Bus zum Steinbruch nach Nussloch gefahren. Nach einer kurzen St\u00e4rkung erhielten wir einen informativen, biologischen Vortrag \u00fcber den Steinbruch von Frau Tina G\u00f6lzer \u2013 sie arbeitet als \u00d6kologin bei HeidelbergCement. Anschlie\u00dfend machten wir uns in Teamarbeit an die Aufgabe, in renaturierten Teilen des Steinbruchs m\u00f6glichst viele verschiedene Pflanzenarten zu sammeln. Jede Pflanzenart wurde von uns fotografiert und die Merkmale in einer Tabelle dokumentiert. Dabei suchten wir zun\u00e4chst auf einer Schwermetallhalde im westlichen Teil des Steinbruchs und daraufhin auf einer nahegelegenen Magerwiese. Nach M\u00f6glichkeit versuchten wir immer, in beiden Habitaten die gleichen Pflanzenarten zu finden, um sie anschlie\u00dfend im EMBL-Labor zu analysieren. Mit vielen in Probenr\u00f6hrchen verpackten Pflanzen im Gepack, machten wir uns nach dem erfolgreichen Tag ersch\u00f6pft auf den Heimweg \u2013 voller Vorfreude auf den nachfolgend geplanten Labortag.<\/em><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nExploring the quarry\u2019s DNA in the laboratory<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>After we had collected various plants for the DNA barcoding at the quarry in Nussloch, we came to the highlight of the project on 13 May 2016: which was the greatly anticipated work in the EMBL laboratory. After a short introduction to the day and a lab safety briefing we started the first wet-lab practicals in the morning. With enthusiasm and in teams of two we set out to extract the DNA from the collected plant samples. Our aim was to amplify specific DNA sequences from the chloroplasts\u2019 genome of the plants by using the polymerase chain reaction (PCR). Small differences in the chloroplast genes allow to genetically discriminate between plant species \u2013 or even sub-species. On this day we concentrated on the rbcL gene. After we refreshed ourselves in the EMBL Cafeteria, we returned to the lab to separate the amplified DNA fragments through gel electrophoresis \u2013 a standard molecular biology procedure to visualize DNA of certain length. Each of us was concentrating on correctly pipetting the amplified DNA fragments onto the gel. Then we watched with great anticipation as to whether our efforts and commitment had paid off. Some results were very promising, others were a bit puzzling. But all in all the results were satisfactory and we sent the DNA off for sequencing to read out the genetic information and to be able to analyse it during the upcoming bioinformatics session.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Die DNA des Steinbruchs<\/em><\/h4>\n\n\n\n<p><em>Nachdem wir am 9. Mai 2016 im Steinbruch in Nussloch diverse Pflanzen f\u00fcr das DNA-Barcoding gesammelt hatten, stand am 13. Mai 2016 das absolute Highlight des Projektes an, n\u00e4mlich die mit voller Vorfreude erwartete Arbeit in den Laboren des EMBL. Um 10:00 Uhr stand nach einer kurzen Einf\u00fchrung und Laborsicherheitsbelehrung der erste Laborteil auf dem Plan. Mit Begeisterung machten wir uns in Zweiergruppen daran, zun\u00e4chst die DNA aus den gesammelten Pflanzenteilen zu extrahieren. Unser Ziel war es, ganz bestimmte DNA bzw. Genabschnitte aus dem Chloroplasten-Genom der Pflanzen mittels der Polymerase-Ketten-Reaktion (PCR) zu vervielf\u00e4ltigen. Kleine Unterschiede in den Genen der Chloroplasten erlauben es n\u00e4mlich, die Pflanzen genetisch einer Art oder sogar Unterart zuzuordnen. Wir konzentrierten uns an diesem Tag auf die rbcL-Fragmente (gro\u00dfe Untereinheit des Ribulose-bisphosphat- carboxylase Gens). Nachdem wir uns in der EMBL-Kantine gest\u00e4rkt hatten, ging es wieder ins Labor, um die in der PCR vervielf\u00e4ltigten Fragmente durch ein Agarosegel elektrophoretisch aufzutrennen. Mit Konzentration war jeder von uns damit besch\u00e4ftigt, die gewonnene DNA unversehrt in die Taschen des Gels zu pipettieren. Anschlie\u00dfend beobachteten wir mit gro\u00dfer (An-) Spannung, ob sich unser Bem\u00fchen und Engagement gelohnt hatte. Einige Ergebnisse waren sehr vielversprechend, andere wiederum zun\u00e4chst etwas r\u00e4tselhaft f\u00fcr uns. Doch alles in allem waren die Resultate zufriedenstellend und wir schickten die DNA zur Sequenzierung, um die darin enthaltene Erbinformation auszulesen und sie dann sp\u00e4ter bioinformatisch bearbeiten zu k\u00f6nnen.<\/em><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nBioinformatics analysis workshop \u2013 the key to species identification<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>The next step in our Quarry Life Award Project was waiting for us on the 2nd of June 2016: the bioinformatics analysis of our plant samples. Meeting at the computer lab of the Marie-Baum-Schule in Heidelberg, we started off with a short review of the lab work we had carried out previously at EMBL. As some of us are still in grade 11 and not yet familiar with the theory of molecular methods such as PCR, we discussed the theoretical basis of this method in greater detail before going on to look at the promising results of our gel electrophoresis. Then, the most important part of the day started: the analysis and interpretation of our DNA sequences. As we had asked the sequencing service to \u201cread\u201d our DNA sequence from both ends, we received two complementary sequences for each PCR product from them. To be able to compare the two, we first used a bioinformatics tool to translate one of the two reads into its reverse complement. We then went on to compare the two reads base by base, resolving any mismatches between them. The final step of the bioinformatics analysis involved searching for a match of our, now error-corrected, DNA sequence (contig) with entries in a biological database (European Nucleotide Archive). After discussing the theory and taking on analysing our real DNA sequences, we all left the session with new knowledge and a great sense of achievement.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Bioinformatik \u2013 der Schl\u00fcssel zur Artenbestimmung<\/em><\/h4>\n\n\n\n<p><em>Am 2. Juni 2016 kamen wir zum n\u00e4chsten Schritt unseres Quarry Life Award Projektes. Dies war der bioinformatische Teil zur Bestimmung der verschiedenen Pflanzenproben. In einem der Computerr\u00e4ume unserer Marie-Baum-Schule in Heidelberg begannen wir mit einer kurzen Wiederholung der Arbeiten bei unserem Laboreinsatz am EMBL. Da manche von uns noch die 11. Klasse besuchen und sich deshalb mit der Methode der PCR noch nicht so gut auskannten, wurde diese noch einmal ausf\u00fchrlich erkl\u00e4rt, sodass nun alle von uns auf dem gleichen Wissenstand waren. Anschlie\u00dfend besprachen wir die Gelelektrophorese, die bereits einige positive Ergebnisse angezeigt hatte. <\/em><em>Dann folgte der wichtigste Teil des Tages \u2013 die bioinformatische Auswertung der Sequenzen. Da bei der Sequenzierung beide DNA-Str\u00e4nge \u201egelesen\u201c werden, erh\u00e4lt man zwei komplement\u00e4re Sequenzen pro PCR-Produkt. Um diese zu vergleichen, muss zun\u00e4chst eine der beiden Sequenzen \u201eumgekehrt\u201c werden (revers komplement\u00e4r). Nun k\u00f6nnen die Sequenzen beider DNA-Str\u00e4nge Baustein f\u00fcr Baustein miteinander verglichen werden. Dies geschieht um unklare Sequenzbereiche zu kl\u00e4ren. Durch Abgleich der DNA-Sequenz mit einer biologischen Datenbank (European Nucleotide Archive) kann man nun mit der korrekturgelesenen Sequenz (Contig) die Artenbestimmung durchf\u00fchren. Nach der Theorie kam die Praxis und wir haben anhand von Beispielen das ganze Verfahren erfolgreich getestet. Somit gingen wir alle mit neuem Wissen und einem Erfolgserlebnis nach Hause.<\/em><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nVisit of the International Jury of the Quarry Life Award 2016<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>On the 21<sup>st<\/sup> July 2016 we had the honour to present our project updates to Professor Rademacher. He is member of the International Jury of the Quarry Life Award 2016, and on this day we met him at EMBL Heidelberg. Our team had prepared everything in advance and were looking forward to present the project. After welcoming our guest, we gave a talk about our project, answered Professor Rademacher\u2019s questions on the methods that we have used and talked about our experiences as participants of the award. We received very positive feedback on our presentation by Professor Rademacher, which we were, of course, happy to hear. After a short coffee break, in which our guest further discussed the project with us, we went to the laboratory. In the lab, we showed him the individual experimental steps involved in DNA barcoding, which we had carried out so far. After presenting our guest with a \u201cBarcode-Sunflower\u201d gift and taking a group picture, it was time to say goodbye. We would like to sincerely thank Professor Rademacher for his interest in the project and the many tips we received from him. \u201cWe hope you enjoyed it as much as we did.\u201d<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Besuch der International Jury des Quarry Life Award 2016<\/em><\/h4>\n\n\n\n<p><em>Am 21. Juli 2016 hatten wir die Ehre, den Stand unseres QLA-Projekts Herrn Professor Rademacher vorzustellen. Er ist Mitglied der internationalen Jury des Quarry Life Award 2016 und wir trafen ihn an diesem Tag am EMBL Heidelberg. Als Team hatten wir im Vorfeld bereits alles vorbereitet und freuten uns auf die Projektvorstellung . Nach einer Begr\u00fc\u00dfung unseres Gastes pr\u00e4sentierten wir unser Projekt zun\u00e4chst in einem Vortrag, beantworteten seine inhaltlichen Fragen zu unserem methodischen Vorgehen und sprachen auch \u00fcber unsere Erfahrungen und Erlebnisse bei der Teilnahme am Wettbewerb. F\u00fcr unsere Pr\u00e4sentation bekamen wir sehr positives Feedback von Herrn Rademacher, \u00fcber welches wir uns nat\u00fcrlich sehr gefreut haben. Nach einer kurzen Kaffeepause, in der sich Professor Rademacher weiter angeregt mit uns unterhielt und uns weitere Fragen \u00fcber unser Projekt stellte, ging es ins Labor. Dort zeigten wir ihm die verschiedenen experimentellen Arbeitsschritte des DNA Barcoding, wie wir sie bereits durchgef\u00fchrt hatten. Nach der \u00dcbergabe unserer \u201eBarcode\u2013Sonnenblume\u201c und einem gemeinsamen Gruppenfoto, endete dieser Einblick in den momentanen Stand unserer Projektarbeit. Wir m\u00f6chten uns noch einmal sehr herzlich bei Professor Rademacher f\u00fcr sein Interesse an unserem Projekt und die zahlreichen Tipps bedanken. \u201eWir hoffen es hat Ihnen genau so viel Freude bereitet wie uns.\u201c<\/em><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nVisit of the National Jury of the Quarry Life Award 2016<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>We were pleased to welcome Ms. Christiane Bohlmann, Head of Marketing at HeidelbergCement Germany and member of the National QLA Jury, on the 15<sup>th<\/sup> September 2016. She visited us in the Advanced Training Centre at EMBL to learn more about the up-to-date status of our QLA project. First, we presented an overview of our project \u201cQuarry biodiversity in focus \u2013 exploring the barcode of life\u201d. She showed great interest and we were very happy to answer all her questions. Before going to the laboratory, we had a short coffee break, which Ms. Bohlmann used to further quiz us about the project and tell us about her experiences when visiting other project presentations. In the lab, we presented and discussed the various experimental steps involved in DNA barcoding. The visit ended with presenting our guest with a \u201cBarcode-Flower\u201d gift and taking a group picture in the sunshine outside. We would like to sincerely thank Ms. Bohlmann for her interest in our project and hope she found the visit as enjoyable as we did.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><em>Besuch der Nationalen Jury des Quarry Life Award 2016<\/em><\/h4>\n\n\n\n<p><em>Mit Frau Christiane Bohlmann, Leiterin des Marketings von HeidelbergCement Deutschland, konnten wir am 15. September 2016 ein Mitglied der nationalen Jury des Quarry Life Award begr\u00fc\u00dfen. Sie stattete uns im Advanced Training Center des EMBL einen Besuch ab, um mehr \u00fcber den Stand unseres QLA-Projektes zu erfahren. Zun\u00e4chst einmal pr\u00e4sentierten wir Frau Bohlmann eine \u00dcbersicht \u00fcber unser Projekt \u201eDie Biodiversit\u00e4t von Abbaust\u00e4tten im Fokus \u2013 dem Barcode des Lebens auf der Spur\u201c. Wir freuten uns sehr, dass Sie nach dem Vortrag viele interessierte Fragen stellte, die wir ihr gerne beantworteten. Bevor wir zusammen ins Labor gingen, legten wir eine kleine Kaffeepause ein, bei der wir uns weiter angeregt unterhielten und Frau Bohlmann uns auch von ihren Erlebnissen bei anderen Projektvorstellungen erz\u00e4hlte. Im Labor stellten wir unserem Gast die verschiedenen experimentellen Arbeitsschritte des DNA Barcoding vor und erkl\u00e4rten diese. Nach dem \u00dcberreichen unserer \u201cBarcode-Blume\u201d gingen wir nach drau\u00dfen, um bei sch\u00f6nstem Wetter ein gemeinsames Gruppenbild zu machen und so den Einblick in unsere Projektarbeit zu beenden. Wir bedanken uns herzlichst bei Frau Bohlmann f\u00fcr Ihr Interesse an unserem Projekt und hoffen es hat ihr genauso viel Spa\u00df gemacht wie uns!<\/em><\/p>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-f70fdb69-09ac-4c31-8fd8-19d39b34acaa\"><h2>Project key dates<\/h2>\n<p><strong>Project coordinator:<\/strong> ELLS<br><strong>Funding: <\/strong>Quarry Life Award, HeidelbergCement<br><strong>Duration: <\/strong>5 months<br><strong>Start:<\/strong> 05\/2016<br><strong>End:<\/strong> 09\/2016<\/p>\n<\/section>\n<\/div><\/div>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"wp-block-gallery columns-2 is-cropped wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\"><ul class=\"blocks-gallery-grid\"><li class=\"blocks-gallery-item\"><figure><img decoding=\"async\" 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