Can a hydroxynitrile lyase catalyze an oxidative cleavage?

Coloma J, Hagedoorn PL, Bento I, Hanefeld U

ACS Catalysis, 2023

doi:10.1021/acscatal.3c02249.

Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans.

Hassani Nia F, Woike D, Bento I, Niebling S, Tibbe D, Schulz K, Hirnet D, Skiba M, Hönck HH, Veith K, Günther C, Scholz T, Bierhals T, Driemeyer J, Bend R, Failla AV, Lohr C, Alai MG, Kreienkamp HJ

Molecular psychiatry, 2022

doi:10.1038/s41380-022-01882-3.

Substrate induced movement of the metal cofactor between active and resting state.

Marsden SR, Wijma HJ, Mohr MKF, Justo I, Hagedoorn PL, Laustsen J, Jeffries CM, Svergun D, Mestrom L, McMillan DGG, Bento I, Hanefeld U

Angewandte Chemie (International ed. in English), 2022

doi:10.1002/anie.202213338.

Structural insights into the pSer/pThr dependent regulation of the SHP2 tyrosine phosphatase in insulin and CD28 signaling.

Zeke A, Takács T, Sok P, Németh K, Kirsch K, Egri P, Póti ÁL, Bento I, Tusnády GE, Reményi A

Nature communications, 2022

doi:10.1038/s41467-022-32918-5.

Cosolvent sites-based discovery of Mycobacterium tuberculosis protein kinase G inhibitors.

Burastero O, Defelipe LA, Gola G, Tateosian NL, Lopez ED, Martinena CB, Arcon JP, Traian MD, Wetzler DE, Bento I, Barril X, Ramirez J, Marti MA, Garcia-Alai MM, Turjanski AG

Journal of medicinal chemistry, 2022

doi:10.1021/acs.jmedchem.1c02012.

A non-catalytic herpesviral protein reconfigures ERK-RSK signaling by targeting kinase docking systems in the host.

Alexa A, Sok P, Gross F, Albert K, Kobori E, Póti ÁL, Gógl G, Bento I, Kuang E, Taylor SS, Zhu F, Ciliberto A, Reményi A

Nature communications, 2022

doi:10.1038/s41467-022-28109-x.

Identification of molecular basis that underlie enzymatic specificity of AzoRo from Rhodococcus opacus 1CP: A potential NADH:quinone oxidoreductase.

Ngo ACR, Qi J, Juric C, Bento I, Tischler D

Archives of biochemistry and biophysics, 2022

doi:10.1016/j.abb.2022.109123.

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease.

Günther S, Reinke PYA, Fernández-García Y, Lieske J, Lane TJ, Ginn HM, Koua FHM, Ehrt C, Ewert W, Oberthuer D, Yefanov O, Meier S, Lorenzen K, Krichel B, Kopicki JD, Gelisio L, Brehm W, Dunkel I, Seychell B, Gieseler H, Norton-Baker B, Escudero-Pérez B, Domaracky M, Saouane S, Tolstikova A, White TA, Hänle A, Groessler M, Fleckenstein H, Trost F, Galchenkova M, Gevorkov Y, Li C, Awel S, Peck A, Barthelmess M, Schluenzen F, Lourdu Xavier P, Werner N, Andaleeb H, Ullah N, Falke S, Srinivasan V, França BA, Schwinzer M, Brognaro H, Rogers C, Melo D, Zaitseva-Doyle JJ, Knoska J, Peña-Murillo GE, Mashhour AR, Hennicke V, Fischer P, Hakanpää J, Meyer J, Gribbon P, Ellinger B, Kuzikov M, Wolf M, Beccari AR, Bourenkov G, von Stetten D, Pompidor G, Bento I, Panneerselvam S, Karpics I, Schneider TR, Garcia-Alai MM, Niebling S, Günther C, Schmidt C, Schubert R, Han H, Boger J, Monteiro DCF, Zhang L, Sun X, Pletzer-Zelgert J, Wollenhaupt J, Feiler CG, Weiss MS, Schulz EC, Mehrabi P, Karničar K, Usenik A, Loboda J, Tidow H, Chari A, Hilgenfeld R, Uetrecht C, Cox R, Zaliani A, Beck T, Rarey M, Günther S, Turk D, Hinrichs W, Chapman HN, Pearson AR, Betzel C, Meents A

Science (New York, N.Y.), 2021

doi:10.1126/science.abf7945.

Immobilization of the highly active UDP-glucose pyrophosphorylase from Thermocrispum agreste provides a highly efficient biocatalyst for the production of UDP-glucose.

Kumpf A, Kowalczykiewicz D, Szymańska K, Mehnert M, Bento I, Łochowicz A, Pollender A, Jarzȩbski A, Tischler D

Frontiers in bioengineering and biotechnology, 2020

doi:10.3389/fbioe.2020.00740.

Co-regulation of the transcription controlling ATF2 phosphoswitch by JNK and p38.

Kirsch K, Zeke A, Tőke O, Sok P, Sethi A, Sebő A, Kumar GS, Egri P, Póti ÁL, Gooley P, Peti W, Bento I, Alexa A, Reményi A

Nature communications, 2020

doi:10.1038/s41467-020-19582-3.

Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis.

Kozak S, Bloch Y, De Munck S, Mikula A, Bento I, Savvides SN, Meijers R

Acta crystallographica. Section D, Structural biology, 2020

doi:10.1107/S2059798320013753.

Anomeric selectivity of trehalose transferase with rare L-sugars.

Mestrom L, Marsden SR, van der Eijk H, Laustsen JU, Jeffries CM, Svergun DI, Hagedoorn PL, Bento I, Hanefeld U

ACS catalysis, 2020

doi:10.1021/acscatal.0c02117.

Two homologous enzymes of the GalU family in Rhodococcus opacus 1CP-RoGalU1 and RoGalU2.

Kumpf A, Partzsch A, Pollender A, Bento I, Tischler D

International journal of molecular sciences, 2019

doi:10.3390/ijms20225809.

Leloir glycosyltransferases in applied biocatalysis: a multidisciplinary approach.

Mestrom L, Przypis M, Kowalczykiewicz D, Pollender A, Kumpf A, Marsden SR, Bento I, Jarzębski AB, Szymańska K, Chruściel A, Tischler D, Schoevaart R, Hanefeld U, Hagedoorn PL

International journal of molecular sciences, 2019

doi:10.3390/ijms20215263.

Correction for Mestrom et al., "Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability".

Mestrom L, Marsden SR, Dieters M, Achterberg P, Stolk L, Bento I, Hanefeld U, Hagedoorn PL

Applied and environmental microbiology, 2019

doi:10.1128/AEM.00942-19.

CH‐π interactions promote the conversion of hydroxypyruvate in a class II pyruvate aldolase.

Marsden SR, Mestrom L, Bento I, Hagedoorn PL, McMillan DGG, Hanefeld U

Advanced Synthesis and Catalysis, 2019

doi:10.1002/adsc.201900205.

Artificial fusion of mCherry enhanced solubility and stability of Trehalose transferase.

Mestrom L, Marsden SR, Dieters M, Achterberg P, Stolk L, Bento I, Hanefeld U, Hagedoorn PL

Applied and environmental microbiology, 2019

doi:10.1128/AEM.03084-18.

P13, the EMBL macromolecular crystallography beamline at the low-emittance PETRA III ring for high- and low-energy phasing with variable beam focusing.

Cianci M, Bourenkov G, Pompidor G, Karpics I, Kallio J, Bento I, Roessle M, Cipriani F, Fiedler S, Schneider TR

Journal of synchrotron radiation, 2017

doi:10.1107/s1600577516016465.

Copper(II) Complexes of Phenanthroline and Histidine Containing Ligands: Synthesis, Characterization and Evaluation of their DNA Cleavage and Cytotoxic Activity

Leite SMG, Lima LMP, Gama S, Mendes F, Orio M, Bento I, Paulo A, Delgado R, Iranzo O

INORGANIC CHEMISTRY, 2016

doi:10.1021/acs.inorgchem.6b01884.

The Aza-Wharton Reaction: Syntheses of Cyclic Allylic Amines and Vicinal Hydroxyamines from the Respective Acylaziridines

Silva S, Rodrigues P, Bento I, Maycock CD

JOURNAL OF ORGANIC CHEMISTRY, 2015

doi:10.1021/jo5029387.

The effect of specific modifications of the amine ligands on the solubility, stability, CO release to myoglobin and whole blood, cell toxicity and haemolytic index of [Mo(CO)(4)(NR3)(2)] complexes

Kromer L, Coelho AC, Bento I, Marques AR, Romao CC

JOURNAL OF ORGANOMETALLIC CHEMISTRY, 2014

doi:10.1016/j.jorganchem.2013.12.009.

Copper(II) and Gallium(III) Complexes of trans-Bis(2-hydroxybenzyl) Cyclen Derivatives: Absence of a Cross-Bridge Proves Surprisingly More Favorable

Esteves CV, Madureira J, Lima LMP, Mateus P, Bento I, Delgado R

INORGANIC CHEMISTRY, 2014

doi:10.1021/ic403156h.

The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis

McVey, C., Ferreira M.J. , Correia B., Lahiri S., de Sanctis D., Carrondo M.A, J., Lindley P., I. de-Sá-Nogueira, Soares C.M., Bento, I.

2014

doi:10.1007/s00775-014-1105-x.

The Crystal Structure of Pseudomonas putida AzoR: the active site revisited

Goncalves AM, Mendes S, de Sanctis D, Martins LO, Bento I

2013

doi:10.1111/febs.12568.

Copper-Catalyzed Regioselective Intramolecular Oxidative a-Functionalization of Tertiary Amines: An Efficient Synthesis of Dihydro-1,3-Oxazines

Deb M. L., Dey S. S., Bento I., Barros M. T. , Maycock C. D.

2013

doi:10.1002/anie.201304654.

Endo-beta-D-1,4-mannanase from Chrysonilia sitophila displays a novel loop arrangement for substrate selectivity

Goncalves AMD, Silva CS, Madeira TI, Coelho R, de Sanctis D, San Romao MV, Bento I

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2012

doi:10.1107/S0907444912034646.

Generation of Carbon Monoxide Releasing Molecules (CO-RMs) as Drug Candidates for the Treatment of Acute Liver Injury: Targeting of CO-RMs to the Liver

Marques AR, Kromer L, Gallo DJ, Penacho N, Rodrigues SS, Seixas JD, Bernardes GJL, Reis PM, Otterbein SL, Ruggieri RA, Goncalves ASG, Goncalves AML, De Matos MN, Bento I, Otterbein LE, Blattler WA, Romao CC

ORGANOMETALLICS, 2012

doi:10.1021/om300360c.

The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: assistance during the proton-transfer mechanism

Silva CS, Damas JM, Chen ZJ, Brissos V, Martins LO, Soares CM, Lindley PF, Bento I

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2012

doi:10.1107/S0907444911054503.

Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of a metallo-oxidase

Silva CS, Durao P, Fillat A, Lindley PF, Martins LO, Bento I

METALLOMICS, 2012

doi:10.1039/c1mt00156f.

The removal of a disulfide bridge in CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability

Fernandes AT, Pereira MM, Silva CS, Lindley PF, Bento I, Melo EP, Martins LO

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2011

doi:10.1007/s00775-011-0768-9.

Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94

Correia B, Chen ZJ, Mendes S, Martins LO, Bento I

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 2011

doi:10.1107/S1744309110048220.

New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases

de Sanctis D, Inacio JM, Lindley PF, de Sa-Nogueira I, Bento I

FEBS JOURNAL, 2010

doi:10.1111/j.1742-4658.2010.07870.x.

Mechanisms underlying dioxygen reduction in laccases. Structural and modelling studies focusing on proton transfer

Bento I, Silva CS, Chen ZJ, Martins LO, Lindley PF, Soares CM

BMC STRUCTURAL BIOLOGY, 2010

doi:10.1186/1472-6807-10-28.

The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis

Chen Z, Durão P, Silva CS, Pereira MM, Todorovic S, Hildebrandt P, Bento I, Lindley PF, Martins LO

2010

doi:10.1039/B922734B.

Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis

de Sanctis D, Bento I, Inacio JM, Custodio S, de Sa-Nogueira I, Carrondo MA

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 2008

doi:10.1107/S1744309108016321.

Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants

Durao P, Chen ZJ, Silva CS, Soares CM, Pereira MM, Todorovic S, Hildebrandt P, Bento I, Lindley PF, Martins LO

BIOCHEMICAL JOURNAL, 2008

doi:10.1042/BJ20080166.

Crystallographic evidence for dioxygen interactions with iron proteins

Carrondo MA, Bento I, Matias PM, Lindley PF

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2007

doi:10.1007/s00775-007-0213-2.

Ceruloplasmin revisited: structural and functional roles of various metal cation-binding sites

Bento I, Peixoto C, Zaitsev VN, Lindley PF

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2007

doi:10.1107/S090744490604947X.

Synthesis and Structural Characterization of 1- and 2-Substituted Indazoles: Ester and Carboxylic Acid Derivatives

Teixeira FC, Ramos H, Antunes IF, Curto MJ, Duarte MT, Bento I

2006

doi:10.3390/11110867.

X-ray structural studies of the fungal laccase from Cerrena maxima

Lyashenko AV, Bento I, Zaitsev VN, Zhukhlistova NE, Zhukova YN, Gabdoulkhakov AG, Morgunova EY, Voelter W, Kachalova GS, Stepanova EV, Koroleva OV, Lamzin VS, Tishkov VI, Betzel C, Lindley PF, Mikhailov AM

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2006

doi:10.1007/s00775-006-0158-x.

Synthesis and structural characterization of 1-and 2-substituted indazoles: Ester and carboxylic acid derivatives

Teixeira FC, Ramos H, Antunes IF, Curto MJM, Duarte MT, Bento I

MOLECULES, 2006

doi:10.3390/11110867.

Purification, crystallization and preliminary X-ray study of the fungal laccase from Cerrena maxima

Lyashenko AV, Zhukhlistova NE, Gabdoulkhakov AG, Zhukova YN, Voelter W, Zaitsev VN, Bento I, Stepanova EV, Kachalova GS, Koroleva OV, Cherkashyn EA, Tishkov VI, Lamzin VS, Schirwitz K, Morgunova EY, Betzel C, Lindley PF, Mikhailov AM

Acta crystallographica. Section F, Structural biology and crystallization communications, 2006

doi:10.1107/s1744309106036578.