Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome.

Meechan K, Guan W, Riedinger A, Stankova V, Yoshimura A, Pipitone R, Milberger A, Schaar H, Romero-Brey I, Templin R, Peddie CJ, Schieber NL, Jones ML, Collinson L, Schwab Y

eLife, 2022

doi:10.7554/elife.80899.

Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies.

Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R

Cell host & microbe, 2020

doi:10.1016/j.chom.2020.11.003.

Lipid droplet quantification based on iterative image processing.

Exner T, Beretta CA, Gao Q, Afting C, Romero-Brey I, Bartenschlager R, Fehring L, Poppelreuther M, Füllekrug J

Journal of lipid research, 2019

doi:10.1194/jlr.D092841.

Spatiotemporal coupling of the hepatitis C virus replication cycle by creating a lipid droplet-proximal membranous replication compartment.

Lee JY, Cortese M, Haselmann U, Tabata K, Romero-Brey I, Funaya C, Schieber NL, Qiang Y, Bartenschlager M, Kallis S, Ritter C, Rohr K, Schwab Y, Ruggieri A, Bartenschlager R

Cell reports, 2019

doi:10.1016/j.celrep.2019.05.063.

An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1.

Exner T, Romero-Brey I, Yifrach E, Rivera-Monroy J, Schrul B, Zouboulis CC, Stremmel W, Honsho M, Bartenschlager R, Zalckvar E, Poppelreuther M, Füllekrug J

Journal of cell science, 2019

doi:10.1242/jcs.223016.

3D electron microscopy (EM) and correlative light electron microscopy (CLEM) methods to study virus-host interactions.

Romero-Brey I

Methods in molecular biology (Clifton, N.J.), 2018

doi:10.1007/978-1-4939-8678-1_11.

Correlative light electron microscopy (CLEM) for tracking and imaging viral protein associated structures in cryo-immobilized cells.

Santarella-Mellwig R, Haselmann U, Schieber NL, Walther P, Schwab Y, Antony C, Bartenschlager R, Romero-Brey I

Journal of visualized experiments : JoVE, 2018

doi:10.3791/58154.

Hepatitis C virus replication depends on endosomal cholesterol homeostasis.

Stoeck IK, Lee JY, Tabata K, Romero-Brey I, Paul D, Schult P, Lohmann V, Kaderali L, Bartenschlager R

Journal of virology, 2018

doi:10.1128/JVI.01196-17.

Membrane alterations induced by nonstructural proteins of human norovirus.

Doerflinger SY, Cortese M, Romero-Brey I, Menne Z, Tubiana T, Schenk C, White PA, Bartenschlager R, Bressanelli S, Hansman GS, Lohmann V

PLoS pathogens, 2017

doi:10.1371/journal.ppat.1006705.

Immune protection against reinfection with nonprimate hepacivirus.

Pfaender S, Walter S, Grabski E, Todt D, Bruening J, Romero-Brey I, Gather T, Brown RJ, Hahn K, Puff C, Pfankuche VM, Hansmann F, Postel A, Becher P, Thiel V, Wagner B, Kalinke U, Baumgärtner W, Bartenschlager R, Pietschmann T, Feige K, Steinmann E, Cavalleri JM

Proceedings of the National Academy of Sciences of the United States of America, 2017

doi:10.1073/pnas.1619380114.

Erratum: Tuning a cellular lipid kinase activity adapts hepatitis C virus to replication in cell culture.

Harak C, Meyrath M, Romero-Brey I, Schenk C, Gondeau C, Schult P, Esser-Nobis K, Saeed M, Neddermann P, Schnitzler P, Gotthardt D, Perez-Del-Pulgar S, Neumann-Haefelin C, Thimme R, Meuleman P, De Francesco R, Florian WR, Bartenschlager R, Rice CM, Lohmann V

Nature microbiology, 2017

doi:10.1038/nmicrobiol.2017.12.

Tuning a cellular lipid kinase activity adapts hepatitis C virus to replication in cell culture.

Harak C, Meyrath M, Romero-Brey I, Schenk C, Gondeau C, Schult P, Esser-Nobis K, Saeed M, Neddermann P, Schnitzler P, Gotthardt D, Perez-Del-Pulgar S, Neumann-Haefelin C, Thimme R, Meuleman P, De Francesco R, Vondran FW, Bartenschlager R, Rice CM, Lohmann V

Nature microbiology, 2016

doi:10.1038/nmicrobiol.2016.247.

Dengue virus perturbs mitochondrial morphodynamics to dampen innate immune responses.

Chatel-Chaix L, Cortese M, Romero-Brey I, Bender S, Neufeldt CJ, Fischl W, Scaturro P, Schieber N, Schwab Y, Fischer B, Ruggieri A, Bartenschlager R

Cell host & microbe, 2016

doi:10.1016/j.chom.2016.07.008.

Endoplasmic reticulum: The favorite intracellular niche for viral replication and assembly.

Romero-Brey I, Bartenschlager R

Viruses, 2016

doi:10.3390/v8060160.

Viral infection at high magnification: 3D electron microscopy methods to analyze the architecture of infected cells.

Romero-Brey I, Bartenschlager R

Viruses, 2015

doi:10.3390/v7122940.

Soraphen A: A broad-spectrum antiviral natural product with potent anti-hepatitis C virus activity.

Koutsoudakis G, Romero-Brey I, Berger C, Pérez-Vilaró G, Monteiro Perin P, Vondran FW, Kalesse M, Harmrolfs K, Müller R, Martinez JP, Pietschmann T, Bartenschlager R, Brönstrup M, Meyerhans A, Díez J

Journal of hepatology, 2015

doi:10.1016/j.jhep.2015.06.002.

Interferon-inducible cholesterol-25-hydroxylase restricts hepatitis C virus replication through blockage of membranous web formation.

Anggakusuma, Romero-Brey I, Berger C, Colpitts CC, Boldanova T, Engelmann M, Todt D, Perin PM, Behrendt P, Vondran FW, Xu S, Goffinet C, Schang LM, Heim MH, Bartenschlager R, Steinmann E, Pietschmann T

Hepatology (Baltimore, Md.), 2015

doi:10.1002/hep.27913.

NS5A domain 1 and polyprotein cleavage kinetics are critical for induction of double-membrane vesicles associated with hepatitis C virus replication.

Romero-Brey I, Berger C, Kallis S, Kolovou A, Paul D, Lohmann V, Bartenschlager R

mBio, 2015

doi:10.1128/mBio.00759-15.

Daclatasvir-like inhibitors of NS5A block early biogenesis of hepatitis C virus-induced membranous replication factories, independent of RNA replication.

Berger C, Romero-Brey I, Radujkovic D, Terreux R, Zayas M, Paul D, Harak C, Hoppe S, Gao M, Penin F, Lohmann V, Bartenschlager R

Gastroenterology, 2014

doi:10.1053/j.gastro.2014.07.019.

Membranous replication factories induced by plus-strand RNA viruses.

Romero-Brey I, Bartenschlager R

Viruses, 2014

doi:10.3390/v6072826.

Three-dimensional architecture of tick-borne encephalitis virus replication sites and trafficking of the replicated RNA.

Miorin L, Romero-Brey I, Maiuri P, Hoppe S, Krijnse-Locker J, Bartenschlager R, Marcello A

Journal of virology, 2013

doi:10.1128/JVI.03456-12.

The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A.

Reiss S, Harak C, Romero-Brey I, Radujkovic D, Klein R, Ruggieri A, Rebhan I, Bartenschlager R, Lohmann V

PLoS pathogens, 2013

doi:10.1371/journal.ppat.1003359.

Analysis of hepatitis C virus resistance to silibinin in vitro and in vivo points to a novel mechanism involving nonstructural protein 4B.

Esser-Nobis K, Romero-Brey I, Ganten TM, Gouttenoire J, Harak C, Klein R, Schemmer P, Binder M, Schnitzler P, Moradpour D, Bartenschlager R, Polyak SJ, Stremmel W, Penin F, Eisenbach C, Lohmann V

Hepatology (Baltimore, Md.), 2013

doi:10.1002/hep.26260.

Three-dimensional architecture and biogenesis of membrane structures associated with hepatitis C virus replication.

Romero-Brey I, Merz A, Chiramel A, Lee JY, Chlanda P, Haselman U, Santarella-Mellwig R, Habermann A, Hoppe S, Kallis S, Walther P, Antony C, Krijnse-Locker J, Bartenschlager R

PLoS pathogens, 2012

doi:10.1371/journal.ppat.1003056.

NS4B self-interaction through conserved C-terminal elements is required for the establishment of functional hepatitis C virus replication complexes.

Paul D, Romero-Brey I, Gouttenoire J, Stoitsova S, Krijnse-Locker J, Moradpour D, Bartenschlager R

Journal of virology, 2011

doi:10.1128/JVI.00502-11.

Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.

Reiss S, Rebhan I, Backes P, Romero-Brey I, Erfle H, Matula P, Kaderali L, Poenisch M, Blankenburg H, Hiet MS, Longerich T, Diehl S, Ramirez F, Balla T, Rohr K, Kaul A, Bühler S, Pepperkok R, Lengauer T, Albrecht M, Eils R, Schirmacher P, Lohmann V, Bartenschlager R

Cell host & microbe, 2011

doi:10.1016/j.chom.2010.12.002.

Genetic analysis of aquabirnaviruses isolated from wild fish reveals occurrence of natural reassortment of infectious pancreatic necrosis virus.

Romero-Brey I, Bandín I, Cutrín JM, Vakharia VN, Dopazo CP

Journal of fish diseases, 2009

doi:10.1111/j.1365-2761.2009.01020.x.

Composition and three-dimensional architecture of the dengue virus replication and assembly sites.

Welsch S, Miller S, Romero-Brey I, Merz A, Bleck CK, Walther P, Fuller SD, Antony C, Krijnse-Locker J, Bartenschlager R

Cell host & microbe, 2009

doi:10.1016/j.chom.2009.03.007.

Molecular characterization of birnaviruses isolated from wild marine fishes at the Flemish Cap (Newfoundland).

Romero-Brey I, Batts WN, Bandín I, Winton JR, Dopazo CP

Diseases of aquatic organisms, 2004

doi:10.3354/dao061001.