Community recommendations on cryoEM data archiving and validation.

Kleywegt GJ, Adams PD, Butcher SJ, Lawson CL, Rohou A, Rosenthal PB, Subramaniam S, Topf M, Abbott S, Baldwin PR, Berrisford JM, Bricogne G, Choudhary P, Croll TI, Danev R, Ganesan SJ, Grant T, Gutmanas A, Henderson R, Heymann JB, Huiskonen JT, Istrate A, Kato T, Lander GC, Lok SM, Ludtke SJ, Murshudov GN, Pye R, Pintilie GD, Richardson JS, Sachse C, Salih O, Scheres SHW, Schroeder GF, Sorzano COS, Stagg SM, Wang Z, Warshamanage R, Westbrook JD, Winn MD, Young JY, Burley SK, Hoch JC, Kurisu G, Morris K, Patwardhan A, Velankar S.

IUCrJ, 2024

doi:10.1107/s2052252524001246.

EMDB-the Electron Microscopy Data Bank.

wwPDB Consortium .

Nucleic Acids Res, 2024

doi:10.1093/nar/gkad1019.

Announcing the launch of Protein Data Bank China as an Associate Member of the Worldwide Protein Data Bank Partnership.

Xu W, Velankar S, Patwardhan A, Hoch JC, Burley SK, Kurisu G.

Acta Crystallogr D Struct Biol, 2023

doi:10.1107/s2059798323006381.

Public archiving of volume EM data.

Iudin A, Hartley M, Kleywegt GJ, Patwardhan A.

Methods Cell Biol, 2023

doi:10.1016/bs.mcb.2023.02.002.

Volume EM: a quiet revolution takes shape.

Collinson LM, Bosch C, Bullen A, Burden JJ, Carzaniga R, Cheng C, Darrow MC, Fletcher G, Johnson E, Narayan K, Peddie CJ, Winn M, Wood C, Patwardhan A, Kleywegt GJ, Verkade P.

Nat Methods, 2023

doi:10.1038/s41592-023-01861-8.

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A.

Nucleic Acids Res, 2023

doi:10.1093/nar/gkac1062.

EMPIAR: The Electron Microscopy Public Image Archive

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A.

2022

doi:10.1101/2022.10.04.510785.

Validation analysis of EMDB entries.

Wang Z, Patwardhan A, Kleywegt GJ.

Acta Crystallogr D Struct Biol, 2022

doi:10.1107/s205979832200328x.

The BioImage Archive - building a home for life-sciences microscopy data

Hartley M, Kleywegt GJ, Patwardhan A, Sarkans U, Swedlow JR, Brazma A.

2021

doi:10.1101/2021.12.17.473169.

3D-Strudel - a novel model-dependent map-feature validation method for high-resolution cryo-EM structures

Istrate A, Wang Z, Murshudov GN, Patwardhan A, Kleywegt GJ.

2021

doi:10.1101/2021.12.16.472999.

Validation analysis of EMDB entries

Wang Z, Patwardhan A, Kleywegt GJ.

2021

doi:10.1101/2021.11.02.466942.

REMBI: Recommended Metadata for Biological Images-enabling reuse of microscopy data in biology.

Sarkans U, Chiu W, Collinson L, Darrow MC, Ellenberg J, Grunwald D, Hériché JK, Iudin A, Martins GG, Meehan T, Narayan K, Patwardhan A, Russell MRG, Saibil HR, Strambio-De-Castillia C, Swedlow JR, Tischer C, Uhlmann V, Verkade P, Barlow M, Bayraktar O, Birney E, Catavitello C, Cawthorne C, Wagner-Conrad S, Duke E, Paul-Gilloteaux P, Gustin E, Harkiolaki M, Kankaanpää P, Lemberger T, McEntyre J, Moore J, Nicholls AW, Onami S, Parkinson H, Parsons M, Romanchikova M, Sofroniew N, Swoger J, Utz N, Voortman LM, Wong F, Zhang P, Kleywegt GJ, Brazma A.

Nat Methods, 2021

doi:10.1038/s41592-021-01166-8.

Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge.

Lawson CL, Kryshtafovych A, Adams PD, Afonine PV, Baker ML, Barad BA, Bond P, Burnley T, Cao R, Cheng J, Chojnowski G, Cowtan K, Dill KA, DiMaio F, Farrell DP, Fraser JS, Herzik MA, Hoh SW, Hou J, Hung LW, Igaev M, Joseph AP, Kihara D, Kumar D, Mittal S, Monastyrskyy B, Olek M, Palmer CM, Patwardhan A, Perez A, Pfab J, Pintilie GD, Richardson JS, Rosenthal PB, Sarkar D, Schäfer LU, Schmid MF, Schröder GF, Shekhar M, Si D, Singharoy A, Terashi G, Terwilliger TC, Vaiana A, Wang L, Wang Z, Wankowicz SA, Williams CJ, Winn M, Wu T, Yu X, Zhang K, Berman HM, Chiu W.

Nat Methods, 2021

doi:10.1038/s41592-020-01051-w.

Outcomes of the 2019 EMDataResource model challenge: validation of cryo-EM models at near-atomic resolution

Lawson CL, Kryshtafovych A, Adams PD, Afonine PV, Baker ML, Barad BA, Bond P, Burnley T, Cao R, Cheng J, Chojnowski G, Cowtan K, Dill KA, DiMaio F, Farrell DP, Fraser JS, Herzik MA, Hoh SW, Hou J, Hung L, Igaev M, Joseph AP, Kihara D, Kumar D, Mittal S, Monastyrskyy B, Olek M, Palmer CM, Patwardhan A, Perez A, Pfab J, Pintilie GD, Richardson JS, Rosenthal PB, Sarkar D, Schäfer LU, Schmid MF, Schröder GF, Shekhar M, Si D, Singharoy A, Terashi G, Terwilliger TC, Vaiana A, Wang L, Wang Z, Wankowicz SA, Williams CJ, Winn M, Wu T, Yu X, Zhang K, Berman HM, Chiu W.

2020

doi:10.1101/2020.06.12.147033.

Comparing Cryo-EM Reconstructions and Validating Atomic Model Fit Using Difference Maps.

Joseph AP, Lagerstedt I, Jakobi A, Burnley T, Patwardhan A, Topf M, Winn M.

J Chem Inf Model, 2020

doi:10.1021/acs.jcim.9b01103.

Protein Data Bank: the single global archive for 3D macromolecular structure data.

wwPDB consortium.

Nucleic Acids Res, 2019

doi:10.1093/nar/gky949.

A call for public archives for biological image data.

Ellenberg J, Swedlow JR, Barlow M, Cook CE, Sarkans U, Patwardhan A, Brazma A, Birney E.

Nat Methods, 2018

doi:10.1038/s41592-018-0195-8.

The first single particle analysis Map Challenge: A summary of the assessments.

Heymann JB, Marabini R, Kazemi M, Sorzano COS, Holmdahl M, Mendez JH, Stagg SM, Jonic S, Palovcak E, Armache JP, Zhao J, Cheng Y, Pintilie G, Chiu W, Patwardhan A, Carazo JM.

J Struct Biol, 2018

doi:10.1016/j.jsb.2018.08.010.

EMDB Web Resources.

Abbott S, Iudin A, Korir PK, Somasundharam S, Patwardhan A.

Curr Protoc Bioinformatics, 2018

doi:10.1002/cpbi.48.

Structural biology data archiving - where we are and what lies ahead.

Kleywegt GJ, Velankar S, Patwardhan A.

FEBS Lett, 2018

doi:10.1002/1873-3468.13086.

Worldwide Protein Data Bank biocuration supporting open access to high-quality 3D structural biology data.

Young JY, Westbrook JD, Feng Z, Peisach E, Persikova I, Sala R, Sen S, Berrisford JM, Swaminathan GJ, Oldfield TJ, Gutmanas A, Igarashi R, Armstrong DR, Baskaran K, Chen L, Chen M, Clark AR, Di Costanzo L, Dimitropoulos D, Gao G, Ghosh S, Gore S, Guranovic V, Hendrickx PMS, Hudson BP, Ikegawa Y, Kengaku Y, Lawson CL, Liang Y, Mak L, Mukhopadhyay A, Narayanan B, Nishiyama K, Patwardhan A, Sahni G, Sanz-García E, Sato J, Sekharan MR, Shao C, Smart OS, Tan L, van Ginkel G, Yang H, Zhuravleva MA, Markley JL, Nakamura H, Kurisu G, Kleywegt GJ, Velankar S, Berman HM, Burley SK.

Database (Oxford), 2018

doi:10.1093/database/bay002.

Validation of Structures in the Protein Data Bank.

Gore S, Sanz García E, Hendrickx PMS, Gutmanas A, Westbrook JD, Yang H, Feng Z, Baskaran K, Berrisford JM, Hudson BP, Ikegawa Y, Kobayashi N, Lawson CL, Mading S, Mak L, Mukhopadhyay A, Oldfield TJ, Patwardhan A, Peisach E, Sahni G, Sekharan MR, Sen S, Shao C, Smart OS, Ulrich EL, Yamashita R, Quesada M, Young JY, Nakamura H, Markley JL, Berman HM, Burley SK, Velankar S, Kleywegt GJ.

Structure, 2017

doi:10.1016/j.str.2017.10.009.

Building bridges between cellular and molecular structural biology.

Patwardhan A, Brandt R, Butcher SJ, Collinson L, Gault D, Grünewald K, Hecksel C, Huiskonen JT, Iudin A, Jones ML, Korir PK, Koster AJ, Lagerstedt I, Lawson CL, Mastronarde D, McCormick M, Parkinson H, Rosenthal PB, Saalfeld S, Saibil HR, Sarntivijai S, Solanes Valero I, Subramaniam S, Swedlow JR, Tudose I, Winn M, Kleywegt GJ.

Elife, 2017

doi:10.7554/elife.25835.

Trends in the Electron Microscopy Data Bank (EMDB).

Patwardhan A.

Acta Crystallogr D Struct Biol, 2017

doi:10.1107/s2059798317004181.

Improved metrics for comparing structures of macromolecular assemblies determined by 3D electron-microscopy.

Joseph AP, Lagerstedt I, Patwardhan A, Topf M, Winn M.

J Struct Biol, 2017

doi:10.1016/j.jsb.2017.05.007.

EMPIAR: Quick tour

Iudin A, Patwardhan A.

2017

doi:10.6019/tol.empiar-qt.2017.00001.1.

OneDep: Unified wwPDB System for Deposition, Biocuration, and Validation of Macromolecular Structures in the PDB Archive.

Young JY, Westbrook JD, Feng Z, Sala R, Peisach E, Oldfield TJ, Sen S, Gutmanas A, Armstrong DR, Berrisford JM, Chen L, Chen M, Di Costanzo L, Dimitropoulos D, Gao G, Ghosh S, Gore S, Guranovic V, Hendrickx PMS, Hudson BP, Igarashi R, Ikegawa Y, Kobayashi N, Lawson CL, Liang Y, Mading S, Mak L, Mir MS, Mukhopadhyay A, Patwardhan A, Persikova I, Rinaldi L, Sanz-Garcia E, Sekharan MR, Shao C, Swaminathan GJ, Tan L, Ulrich EL, van Ginkel G, Yamashita R, Yang H, Zhuravleva MA, Quesada M, Kleywegt GJ, Berman HM, Markley JL, Nakamura H, Velankar S, Burley SK.

Structure, 2017

doi:10.1016/j.str.2017.01.004.

New insights into the GINS complex explain the controversy between existing structural models.

Carroni M, De March M, Medagli B, Krastanova I, Taylor IA, Amenitsch H, Araki H, Pisani FM, Patwardhan A, Onesti S.

Sci Rep, 2017

doi:10.1038/srep40188.

Databases and Archiving for CryoEM.

Patwardhan A, Lawson CL.

Methods Enzymol, 2016

doi:10.1016/bs.mie.2016.04.015.

EMPIAR: a public archive for raw electron microscopy image data.

Iudin A, Korir PK, Salavert-Torres J, Kleywegt GJ, Patwardhan A.

Nat Methods, 2016

doi:10.1038/nmeth.3806.

Web-based volume slicer for 3D electron-microscopy data from EMDB.

Salavert-Torres J, Iudin A, Lagerstedt I, Sanz-García E, Kleywegt GJ, Patwardhan A.

J Struct Biol, 2016

doi:10.1016/j.jsb.2016.02.012.

The Electron Microscopy eXchange (EMX) initiative.

Marabini R, Ludtke SJ, Murray SC, Chiu W, de la Rosa-Trevín JM, Patwardhan A, Heymann JB, Carazo JM.

J Struct Biol, 2016

doi:10.1016/j.jsb.2016.02.008.

EMDataBank unified data resource for 3DEM.

Lawson CL, Patwardhan A, Baker ML, Hryc C, Garcia ES, Hudson BP, Lagerstedt I, Ludtke SJ, Pintilie G, Sala R, Westbrook JD, Berman HM, Kleywegt GJ, Chiu W.

Nucleic Acids Res, 2016

doi:10.1093/nar/gkv1126.

PDBe: improved accessibility of macromolecular structure data from PDB and EMDB.

Velankar S, van Ginkel G, Alhroub Y, Battle GM, Berrisford JM, Conroy MJ, Dana JM, Gore SP, Gutmanas A, Haslam P, Hendrickx PM, Lagerstedt I, Mir S, Fernandez Montecelo MA, Mukhopadhyay A, Oldfield TJ, Patwardhan A, Sanz-García E, Sen S, Slowley RA, Wainwright ME, Deshpande MS, Iudin A, Sahni G, Salavert Torres J, Hirshberg M, Mak L, Nadzirin N, Armstrong DR, Clark AR, Smart OS, Korir PK, Kleywegt GJ.

Nucleic Acids Res, 2016

doi:10.1093/nar/gkv1047.

Outcome of the First wwPDB Hybrid/Integrative Methods Task Force Workshop.

Sali A, Berman HM, Schwede T, Trewhella J, Kleywegt G, Burley SK, Markley J, Nakamura H, Adams P, Bonvin AM, Chiu W, Peraro MD, Di Maio F, Ferrin TE, Grünewald K, Gutmanas A, Henderson R, Hummer G, Iwasaki K, Johnson G, Lawson CL, Meiler J, Marti-Renom MA, Montelione GT, Nilges M, Nussinov R, Patwardhan A, Rappsilber J, Read RJ, Saibil H, Schröder GF, Schwieters CD, Seidel CA, Svergun D, Topf M, Ulrich EL, Velankar S, Westbrook JD.

Structure, 2015

doi:10.1016/j.str.2015.05.013.

Collaborative computational project for electron cryo-microscopy.

Wood C, Burnley T, Patwardhan A, Scheres S, Topf M, Roseman A, Winn M.

Acta Crystallogr D Biol Crystallogr, 2015

doi:10.1107/s1399004714018070.

A 3D cellular context for the macromolecular world.

Patwardhan A, Ashton A, Brandt R, Butcher S, Carzaniga R, Chiu W, Collinson L, Doux P, Duke E, Ellisman MH, Franken E, Grünewald K, Heriche JK, Koster A, Kühlbrandt W, Lagerstedt I, Larabell C, Lawson CL, Saibil HR, Sanz-García E, Subramaniam S, Verkade P, Swedlow JR, Kleywegt GJ.

Nat Struct Mol Biol, 2014

doi:10.1038/nsmb.2897.

PDBe: Protein Data Bank in Europe.

Gutmanas A, Alhroub Y, Battle GM, Berrisford JM, Bochet E, Conroy MJ, Dana JM, Fernandez Montecelo MA, van Ginkel G, Gore SP, Haslam P, Haslam P, Hatherley R, Hendrickx PM, Hirshberg M, Lagerstedt I, Mir S, Mukhopadhyay A, Oldfield TJ, Patwardhan A, Rinaldi L, Sahni G, Sanz-García E, Sanz-García E, Sen S, Slowley RA, Velankar S, Wainwright ME, Kleywegt GJ.

Nucleic Acids Res, 2014

doi:10.1093/nar/gkt1180.

Emdatabank: Unified Data Resource for 3DEM

Catherine Lawson, Ardan Patwardhan, Grigore D Pintilie, Eduardo Sanz Garcia, Ingvar Lagerstedt, Matthew L Baker, Raul Sala, Steven J Ludtke, Helen M Berman, Gerard Kleywegt, Wah Chiu.

Biophys J, 2013

doi:10.1016/j.bpj.2012.11.1950.

Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB.

Lagerstedt I, Moore WJ, Patwardhan A, Sanz-García E, Best C, Swedlow JR, Kleywegt GJ.

J Struct Biol, 2013

doi:10.1016/j.jsb.2013.09.021.

The role of structural bioinformatics resources in the era of integrative structural biology.

Gutmanas A, Oldfield TJ, Patwardhan A, Sen S, Velankar S, Kleywegt GJ.

Acta Crystallogr D Biol Crystallogr, 2013

doi:10.1107/s0907444913001157.

Cryo-electron microscopy--a primer for the non-microscopist.

Milne JL, Borgnia MJ, Bartesaghi A, Tran EE, Earl LA, Schauder DM, Lengyel J, Pierson J, Patwardhan A, Subramaniam S.

FEBS J, 2013

doi:10.1111/febs.12078.

Data management challenges in three-dimensional EM.

Patwardhan A, Carazo JM, Carragher B, Henderson R, Heymann JB, Hill E, Jensen GJ, Lagerstedt I, Lawson CL, Ludtke SJ, Mastronarde D, Moore WJ, Roseman A, Rosenthal P, Sorzano CO, Sanz-García E, Scheres SH, Subramaniam S, Westbrook J, Winn M, Swedlow JR, Kleywegt GJ.

Nat Struct Mol Biol, 2012

doi:10.1038/nsmb.2426.

OMERO: flexible, model-driven data management for experimental biology.

Allan C, Burel JM, Moore J, Blackburn C, Linkert M, Loynton S, Macdonald D, Moore WJ, Neves C, Patterson A, Porter M, Tarkowska A, Loranger B, Avondo J, Lagerstedt I, Lianas L, Leo S, Hands K, Hay RT, Patwardhan A, Best C, Kleywegt GJ, Zanetti G, Swedlow JR.

Nat Methods, 2012

doi:10.1038/nmeth.1896.

PDBe: Protein Data Bank in Europe.

Velankar S, Alhroub Y, Best C, Caboche S, Conroy MJ, Dana JM, Fernandez Montecelo MA, van Ginkel G, Golovin A, Gore SP, Gutmanas A, Haslam P, Hendrickx PM, Heuson E, Hirshberg M, John M, Lagerstedt I, Mir S, Newman LE, Oldfield TJ, Patwardhan A, Rinaldi L, Sahni G, Sanz-García E, Sen S, Slowley R, Suarez-Uruena A, Swaminathan GJ, Symmons MF, Vranken WF, Wainwright M, Kleywegt GJ.

Nucleic Acids Res, 2012

doi:10.1093/nar/gkr998.

Atomic resolution insights into curli fiber biogenesis.

Taylor JD, Zhou Y, Salgado PS, Patwardhan A, McGuffie M, Pape T, Grabe G, Ashman E, Constable SC, Simpson PJ, Lee WC, Cota E, Chapman MR, Matthews SJ.

Structure, 2011

doi:10.1016/j.str.2011.05.015.

Mutations in subdomain B of the minichromosome maintenance (MCM) helicase affect DNA binding and modulate conformational transitions.

Jenkinson ER, Costa A, Leech AP, Patwardhan A, Onesti S, Chong JP.

J Biol Chem, 2009

doi:10.1074/jbc.m806973200.

Cryo-electron microscopy reveals a novel DNA-binding site on the MCM helicase.

Costa A, van Duinen G, Medagli B, Chong J, Sakakibara N, Kelman Z, Nair SK, Patwardhan A, Onesti S.

EMBO J, 2008

doi:10.1038/emboj.2008.135.

A novel mirror-symmetry analysis approach for the study of macromolecular assemblies imaged by electron microscopy.

Costa A, Patwardhan A.

J Mol Biol, 2008

doi:10.1016/j.jmb.2008.02.054.

Different quaternary structures of human RECQ1 are associated with its dual enzymatic activity.

Muzzolini L, Beuron F, Patwardhan A, Popuri V, Cui S, Niccolini B, Rappas M, Freemont PS, Vindigni A.

PLoS Biol, 2007

doi:10.1371/journal.pbio.0050020.

Structural basis of the Methanothermobacter thermautotrophicus MCM helicase activity.

Costa A, Pape T, van Heel M, Brick P, Patwardhan A, Onesti S.

Nucleic Acids Res, 2006

doi:10.1093/nar/gkl708.

Structural studies of the archaeal MCM complex in different functional states.

Costa A, Pape T, van Heel M, Brick P, Patwardhan A, Onesti S.

J Struct Biol, 2006

doi:10.1016/j.jsb.2006.04.001.

Magnification variations due to illumination curvature and object defocus in transmission electron microscopy.

van Duinen G, van Heel M, Patwardhan A.

Opt Express, 2005

doi:10.1364/opex.13.009085.

Conformational changes of Escherichia coli sigma54-RNA-polymerase upon closed-promoter complex formation.

Ray P, Hall RJ, Finn RD, Chen S, Patwardhan A, Buck M, van Heel M.

J Mol Biol, 2005

doi:10.1016/j.jmb.2005.09.057.

A measure for the angle between projections based on the extent of correlation between corresponding central sections.

Patwardhan A, Paul D, Al-Khayat HA, Morris EP.

J Mol Biol, 2004

doi:10.1016/j.jmb.2004.09.068.

Single particle analysis of filamentous and highly elongated macromolecular assemblies.

Paul D, Patwardhan A, Squire JM, Squire JM, Morris EP.

J Struct Biol, 2004

doi:10.1016/j.jsb.2004.05.004.

A two step approach for semi-automated particle selection from low contrast cryo-electron micrographs.

Hall RJ, Patwardhan A.

J Struct Biol, 2004

doi:10.1016/j.jsb.2003.10.024.

Transmission electron microscopy of weakly scattering objects described by operator algebra.

Patwardhan A.

J Opt Soc Am A Opt Image Sci Vis, 2003

doi:10.1364/josaa.20.001210.

Single-particle electron cryo-microscopy: towards atomic resolution.

van Heel M, Gowen B, Matadeen R, Orlova EV, Finn R, Pape T, Cohen D, Stark H, Schmidt R, Schatz M, Patwardhan A.

Q Rev Biophys, 2000

doi:10.1017/s0033583500003644.

The Escherichia coli large ribosomal subunit at 7.5 A resolution.

Matadeen R, Patwardhan A, Gowen B, Orlova EV, Pape T, Cuff M, Mueller F, Brimacombe R, van Heel M.

Structure, 1999

doi:10.1016/s0969-2126(00)88348-3.

Reconstructed human epidermis composed of keratinocytes, melanocytes and Langerhans cells.

Régnier M, Patwardhan A, Scheynius A, Schmidt R.

Med Biol Eng Comput, 1998

doi:10.1007/bf02518889.

HLA-DM is expressed on the cell surface and colocalizes with HLA-DR and invariant chain in human Langerhans cells.

Andersson T, Patwardhan A, Emilson A, Carlsson K, Scheynius A.

Arch Dermatol Res, 1998

doi:10.1007/s004030050372.

A study of the precision of confocal, ratiometric, Fura-2-based [Ca2+] measurements.

Helm PJ, Patwardhan A, Manders EM.

Cell Calcium, 1997

doi:10.1016/s0143-4160(97)90067-1.

[Transport and localization of troxerutin in the venous wall].

Carlsson K, Patwardhan A, Poullain JC, Gerentes I.

J Mal Vasc, 1996

doi:.

The affinity of troxerutin for the venous wall measured by laser scanning microscopy.

Patwardhan A, Carlsson K, Poullain JC, Taccoen A, Gerentes I.

J Cardiovasc Surg (Torino), 1995

doi:.