Structural and functional insights into tRNA recognition by human tRNA guanine transglycosylase.

Sievers K, Neumann P, Sušac L, Da Vela S, Graewert M, Trowitzsch S, Svergun D, Tampé R, Ficner R

Structure (London, England : 1993), 2023

doi:10.1016/j.str.2023.12.006.

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS).

Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D

Communications biology, 2023

doi:10.1038/s42003-023-05416-7.

Vibrio cholerae's ToxRS bile sensing system.

Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T

eLife, 2023

doi:10.7554/elife.88721.

Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering.

Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

Scientific reports, 2023

doi:10.1038/s41598-023-42274-z.

Small conformational changes in IgG1 detected as acidic charge variants by cation exchange chromatography.

Fukuda M, Graewert MA, Jeffries CM, Svergun DI, Yamazaki T, Koga A, Yamanaka Y

Analytical biochemistry, 2023

doi:10.1016/j.ab.2023.115302.

Structural and functional analysis of a plant nucleolar RNA chaperone-like protein.

Fernandes R, Ostendorp A, Ostendorp S, Mehrmann J, Falke S, Graewert MA, Weingartner M, Kehr J, Hoth S

Scientific reports, 2023

doi:10.1038/s41598-023-36426-4.

Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein.

Sacerdoti M, Gross LZF, Riley AM, Zehnder K, Ghode A, Klinke S, Anand GS, Paris K, Winkel A, Herbrand AK, Godage HY, Cozier GE, Süß E, Schulze JO, Pastor-Flores D, Bollini M, Cappellari MV, Svergun D, Gräwert MA, Aramendia PF, Leroux AE, Potter BVL, Camacho CJ, Biondi RM

Science signaling, 2023

doi:10.1126/scisignal.add3184.

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking.

Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland TE, Cowieson N, Crossett B, Duff AP, Franke D, Gabel F, Gillilan RE, Graewert M, Grishaev A, Guss JM, Hammel M, Hopkins J, Huang Q, Hub JS, Hura GL, Irving TC, Jeffries CM, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg DJ, Ryan TM, Seifert S, Sekiguchi H, Svergun D, Teixeira S, Thureau A, Weiss TM, Whitten AE, Wood K, Zuo X

Acta crystallographica. Section D, Structural biology, 2022

doi:10.1107/s2059798322009184.

Advanced sample environments and sample requirements for biological SAXS.

Graewert MA, Svergun DI

Methods in enzymology, 2022

doi:10.1016/bs.mie.2022.08.009.

Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF.

Corbeski I, Guo X, Eckhardt BV, Fasci D, Wiegant W, Graewert MA, Vreeken K, Wienk H, Svergun DI, Heck AJR, van Attikum H, Boelens R, Sixma TK, Mattiroli F, van Ingen H

Science advances, 2022

doi:10.1126/sciadv.abo0517.

EFAMIX, a tool to decompose inline chromatography SAXS data from partially overlapping components.

Konarev PV, Graewert MA, Jeffries CM, Fukuda M, Cheremnykh TA, Volkov VV, Svergun DI

Protein science : a publication of the Protein Society, 2021

doi:10.1002/pro.4237.

The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding.

Bernardini A, Lorenzo M, Chaves-Sanjuan A, Swuec P, Pigni M, Saad D, Konarev PV, Graewert MA, Valentini E, Svergun DI, Nardini M, Mantovani R, Gnesutta N

International journal of biological macromolecules, 2021

doi:10.1016/j.ijbiomac.2021.10.056.

Molecular basis of F-actin regulation and sarcomere assembly via myotilin.

Kostan J, Pavšič M, Puž V, Schwarz TC, Drepper F, Molt S, Graewert MA, Schreiner C, Sajko S, van der Ven PFM, Onipe A, Svergun DI, Warscheid B, Konrat R, Fürst DO, Lenarčič B, Djinović-Carugo K

PLoS biology, 2021

doi:10.1371/journal.pbio.3001148.

The allosteric modulation of Complement C5 by knob domain peptides.

Macpherson A, Laabei M, Ahdash Z, Graewert MA, Birtley JR, Schulze ME, Crennell S, Robinson SA, Holmes B, Oleinikovas V, Nilsson PH, Snowden J, Ellis V, Mollnes TE, Deane CM, Svergun D, Lawson AD, van den Elsen JM

eLife, 2021

doi:10.7554/eLife.63586.

Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.

Sajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K

PloS one, 2020

doi:10.1371/journal.pone.0242677.

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.

Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer MA, Gruzinov AY, Jeffries CM, Graewert MA, Svergun DI, Dobrev N, Remans K, Seeger MA, McInerney GM, Murrell B, Hällberg BM, Löw C

Nature communications, 2020

doi:10.1038/s41467-020-19204-y.

Adding size exclusion chromatography (SEC) and light scattering (LS) devices to obtain high-quality small angle X-ray scattering (SAXS) data.

Graewert MA, Da Vela S, Gräwert TW, Molodenskiy DS, Blanchet CE, Svergun DI, Jeffries CM

Crystals, 2020

doi:10.3390/cryst10110975.

Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.

Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A

Proceedings of the National Academy of Sciences of the United States of America, 2019

doi:10.1073/pnas.1913690116.

Conformational characterization of full-length X-chromosome-linked inhibitor of apoptosis protein (XIAP) through an integrated approach.

Polykretis P, Luchinat E, Bonucci A, Giachetti A, Graewert MA, Svergun DI, Banci L

IUCrJ, 2019

doi:10.1107/S205225251901073X.

The quaternary structure of insulin glargine and glulisine under formulation conditions.

Nagel N, Graewert MA, Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold, H.

Biophysical Chemistry, 2019

doi:10.1016/j.bpc.2019.106226.

Utilization of staphylococcal immune evasion protein Sbi as a novel vaccine adjuvant.

Yang Y, Back CR, Gräwert MA, Wahid AA, Denton H, Kildani R, Paulin J, Wörner K, Kaiser W, Svergun DI, Sartbaeva A, Watts AG, Marchbank KJ, van den Elsen JMH

Frontiers in immunology, 2018

doi:10.3389/fimmu.2018.03139.

Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions.

Schroer MA, Blanchet CE, Gruzinov AY, Gräwert MA, Brennich ME, Hajizadeh NR, Jeffries CM, Svergun DI

Journal of Synchrotron Radiation, 2018

doi:10.1107/S1600577518007907.

Multi-channel in situ dynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12.

Falke S, Dierks K, Blanchet C, Graewert M, Cipriani F, Meijers R, Svergun D, Betzel C

Journal of synchrotron radiation, 2018

doi:10.1107/S1600577517017568.

Sample and Buffer Preparation for SAXS.

Graewert MA, Jeffries CM

Advances in experimental medicine and biology, 2017

doi:10.1007/978-981-10-6038-0_2.

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments.

Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI

Nature protocols, 2016

doi:10.1038/nprot.2016.113.

Characterization of mAb dimers reveals predominant dimer forms common in therapeutic mAbs.

Plath F, Ringler P, Graff-Meyer A, Stahlberg H, Lauer ME, Rufer AC, Graewert MA, Svergun D, Gellermann G, Finkler C, Stracke JO, Koulov A, Schnaible V

mAbs, 2016

doi:10.1080/19420862.2016.1168960.

LabDisk for SAXS: a centrifugal microfluidic sample preparation platform for small-angle X-ray scattering.

Schwemmer F, Blanchet CE, Spilotros A, Kosse D, Zehnle S, Mertens HD, Graewert MA, Rössle M, Paust N, Svergun DI, von Stetten F, Zengerle R, Mark D

Lab on a chip, 2016

doi:10.1039/c5lc01580d.

Structural characterization of a Vatairea macrocarpa lectin in complex with a tumor-associated antigen: a new tool for cancer research.

Sousa BL, Silva-Filho JC, Kumar P, Graewert MA, Pereira RI, Cunha RM, Nascimento KS, Bezerra GA, Delatorre P, Djinovic-Carugo K, Nagano CS, Gruber K, Cavada BS

The international journal of biochemistry & cell biology, 2016

doi:10.1016/j.biocel.2015.12.016.

Structural basis for antigen recognition by transglutaminase 2-specific autoantibodies in celiac disease.

Chen X, Hnida K, Graewert MA, Andersen JT, Iversen R, Tuukkanen A, Svergun D, Sollid LM

The Journal of biological chemistry, 2015

doi:10.1074/jbc.M115.669895.

Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.

Graewert MA, Franke D, Jeffries CM, Blanchet CE, Ruskule D, Kuhle K, Flieger A, Schäfer B, Tartsch B, Meijers R, Svergun DI

Scientific reports, 2015

doi:10.1038/srep10734.

Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY).

Blanchet CE, Spilotros A, Schwemmer F, Graewert MA, Kikhney A, Jeffries CM, Franke D, Mark D, Zengerle R, Cipriani F, Fiedler S, Roessle M, Svergun DI

Journal of applied crystallography, 2015

doi:10.1107/s160057671500254x.