Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response.

Ruperti F, Becher I, Stokkermans A, Wang L, Marschlich N, Potel C, Maus E, Stein F, Drotleff B, Schippers KJ, Nickel M, Prevedel R, Musser JM, Savitski MM, Arendt D

Current biology : CB, 2023

doi:10.1016/j.cub.2023.12.021.

STPP-UP: an alternative method for drug target identification using protein thermal stability.

Zijlmans DW, Hernández-Quiles M, Jansen PWTC, Becher I, Stein F, Savitski MM, Vermeulen M

The Journal of biological chemistry, 2023

doi:10.1016/j.jbc.2023.105279.

Dual truncation of tau by caspase-2 accelerates its CHIP-mediated degradation.

Reinhardt L, Musacchio F, Bichmann M, Behrendt A, Ercan-Herbst E, Stein J, Becher I, Haberkant P, Mader J, Schöndorf DC, Schmitt M, Korffmann J, Reinhardt P, Pohl C, Savitski M, Klein C, Gasparini L, Fuhrmann M, Ehrnhoefer DE

Neurobiology of disease, 2023

doi:10.1016/j.nbd.2023.106126.

Defining basic rules for hardening influenza A virus liquid condensates.

Etibor TA, Vale-Costa S, Sridharan S, Brás D, Becher I, Mello VH, Ferreira F, Alenquer M, Savitski MM, Amorim MJ

eLife, 2023

doi:10.7554/elife.85182.

Molecular mechanisms of stress-induced reactivation in mumps virus condensates.

Zhang X, Sridharan S, Zagoriy I, Eugster Oegema C, Ching C, Pflaesterer T, Fung HKH, Becher I, Poser I, Müller CW, Hyman AA, Savitski MM, Mahamid J

Cell, 2023

doi:10.1016/j.cell.2023.03.015.

Deep thermal profiling for detection of functional proteoform groups.

Kurzawa N, Leo IR, Stahl M, Kunold E, Becher I, Audrey A, Mermelekas G, Huber W, Mateus A, Savitski MM, Jafari R

Nature chemical biology, 2023

doi:10.1038/s41589-023-01284-8.

Author Correction: A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles.

Kurzawa N, Becher I, Sridharan S, Franken H, Mateus A, Anders S, Bantscheff M, Huber W, Savitski MM

Nature communications, 2021

doi:10.1038/s41467-021-27561-5.

Impact of phosphorylation on thermal stability of proteins.

Potel CM, Kurzawa N, Becher I, Typas A, Mateus A, Savitski MM

Nature methods, 2021

doi:10.1038/s41592-021-01177-5.

Cell surface thermal proteome profiling tracks perturbations and drug targets on the plasma membrane.

Kalxdorf M, Günthner I, Becher I, Kurzawa N, Knecht S, Savitski MM, Eberl HC, Bantscheff M

Nature methods, 2021

doi:10.1038/s41592-020-01022-1.

A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles.

Kurzawa N, Becher I, Sridharan S, Franken H, Mateus A, Anders S, Bantscheff M, Huber W, Savitski MM

Nature communications, 2020

doi:10.1038/s41467-020-19529-8.

Meltome atlas-thermal proteome stability across the tree of life.

Jarzab A, Kurzawa N, Hopf T, Moerch M, Zecha J, Leijten N, Bian Y, Musiol E, Maschberger M, Stoehr G, Becher I, Daly C, Samaras P, Mergner J, Spanier B, Werner T, Angelov A, Wilhelm M, Bantscheff M, Lemeer S, Klingenspor M, Hahne H, Liebl W, Kuster B, Savitski MM

Nature methods, 2020

doi:10.1038/s41592-020-0801-4.

Thermal proteome profiling for interrogating protein interactions.

Mateus A, Kurzawa N, Becher I, Sridharan S, Helm D, Stein F, Typas A, Savitski MM

Molecular systems biology, 2020

doi:10.15252/msb.20199232.

Identifying drug targets in tissues and whole blood with thermal-shift profiling.

Perrin J, Werner T, Kurzawa N, Rutkowska A, Childs DD, Kalxdorf M, Poeckel D, Stonehouse E, Strohmer K, Heller B, Thomson DW, Krause J, Becher I, Eberl HC, Vappiani J, Rau CE, Sevin DC, Huber W, Franken H, Savitski MM, Faelth-Savitski M, Bergamini G, Bantscheff M

Nature biotechnology, 2020

doi:10.1038/s41587-019-0388-4.

Impact of phosphorylation on thermal stability of proteins.

Potel CM, Kurzawa N, Becher I, Typas A, Mateus A, Savitski MM

bioRxiv, 2020

doi:10.1101/2020.01.14.903849.

Target discovery using thermal proteome profiling.

Sridharan S, Günthner I, Becher I, Savitski M, Bantscheff M

2019

doi:10.1002/9781118970195.ch11.

Pervasive protein thermal stability variation during the cell cycle.

Becher I, Andrés-Pons A, Romanov N, Stein F, Schramm M, Baudin F, Helm D, Kurzawa N, Mateus A, Mackmull MT, Typas A, Müller CW, Bork P, Beck M, Savitski MM

Cell, 2018

doi:10.1016/j.cell.2018.03.053.

Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis.

Savitski MM, Zinn N, Faelth-Savitski M, Poeckel D, Gade S, Becher I, Muelbaier M, Wagner AJ, Strohmer K, Werner T, Melchert S, Petretich M, Rutkowska A, Vappiani J, Franken H, Steidel M, Sweetman GM, Gilan O, Lam EYN, Dawson MA, Prinjha RK, Grandi P, Bergamini G, Bantscheff M

Cell, 2018

doi:10.1016/j.cell.2018.02.030.

Systematic analysis of protein turnover in primary cells.

Mathieson T, Franken H, Kosinski J, Kurzawa N, Zinn N, Sweetman G, Poeckel D, Ratnu VS, Schramm M, Becher I, Steidel M, Noh KM, Bergamini G, Beck M, Bantscheff M, Savitski MM

Nature communications, 2018

doi:10.1038/s41467-018-03106-1.

Thermal profiling reveals phenylalanine hydroxylase as an off-target of panobinostat.

Becher I, Werner T, Doce C, Zaal EA, Tögel I, Khan CA, Rueger A, Muelbaier M, Salzer E, Berkers CR, Fitzpatrick PF, Bantscheff M, Savitski MM

Nature chemical biology, 2016

doi:10.1038/nchembio.2185.

Functional interdependence of BRD4 and DOT1L in MLL leukemia.

Gilan O, Lam EYN, Becher I, Lugo D, Cannizzaro E, Joberty G, Ward A, Wiese M, Fong CY, Ftouni S, Tyler D, Stanley K, MacPherson L, Weng CF, Chan YC, Ghisi M, Smil D, Carpenter C, Brown P, Garton N, Blewitt ME, Bannister AJ, Kouzarides T, Huntly BJP, Johnstone RW, Drewes G, Dawson SJ, Arrowsmith CH, Grandi P, Prinjha RK, Dawson MA, Dawson MA

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2016

doi:10.1038/nsmb.3249.

GSK6853, a chemical probe for inhibition of the BRPF1 bromodomain.

Bamborough P, Barnett HA, Becher I, Bird MJ, Chung CW, Craggs PD, Demont EH, Diallo H, Fallon DJ, Gordon LJ, Grandi P, Hobbs CI, Hooper-Greenhill E, Jones EJ, Law RP, Le Gall A, Lugo D, Michon AM, Mitchell DJ, Prinjha RK, Sheppard RJ, Watson AJB, Watson RJ, Watson RJ

ACS MEDICINAL CHEMISTRY LETTERS, 2016

doi:10.1021/acsmedchemlett.6b00092.

Discovery of I-BRD9, a selective cell active chemical probe for bromodomain containing protein 9 inhibition.

Theodoulou NH, Bamborough P, Bannister AJ, Becher I, Bit RA, Che KH, Chung CW, Dittmann A, Drewes G, Drewry DH, Gordon L, Grandi P, Leveridge M, Lindon M, Michon AM, Molnar J, Robson SC, Tomkinson NCO, Kouzarides T, Prinjha RK, Humphreys PG, Humphreys PG

JOURNAL OF MEDICINAL CHEMISTRY, 2015

doi:10.1021/acs.jmedchem.5b00256.

Discovery of I-BRD9, a Selective Cell Active Chemical Probe for Bromodomain Containing Protein 9 Inhibition.

Theodoulou NH, Bamborough P, Bannister AJ, Becher I, Bit RA, Che KH, Chung CW, Dittmann A, Drewes G, Drewry DH, Gordon L, Grandi P, Leveridge M, Lindon M, Michon AM, Molnar J, Robson SC, Tomkinson NC, Kouzarides T, Prinjha RK, Humphreys PG

Journal of medicinal chemistry, 2015

doi:10.1021/acs.jmedchem.5b00256.

Ion Mobility Tandem Mass Spectrometry Enhances Performance of Bottom-up Proteomics

Helm D, Vissers JPC, Hughes CJ, Hahne H, Ruprecht B, Pachl F, Grzyb A, Richardson K, Wildgoose J, Maier SK, Marx H, Wilhelm M, Becher I, Lemeer S, Bantscheff M, Langridge JI, Kuster B

MOLECULAR & CELLULAR PROTEOMICS, 2014

doi:10.1074/mcp.M114.041038.

Chemoproteomics reveals time-dependent binding of histone deacetylase inhibitors to endogenous repressor complexes.

Becher I, Dittmann A, Savitski MM, Hopf C, Drewes G, Bantscheff M

ACS chemical biology, 2014

doi:10.1021/cb500235n.

Quantifying small molecule-induced changes in cellular protein expression and posttranslational modifications using isobaric mass tags.

Becher I, Savitski MF, Bantscheff M

Methods in molecular biology (Clifton, N.J.), 2014

doi:10.1007/978-1-4939-0685-7_29.

Measuring and managing ratio compression for accurate iTRAQ/TMT quantification.

Savitski MM, Mathieson T, Zinn N, Sweetman G, Doce C, Becher I, Pachl F, Kuster B, Bantscheff M

Journal of proteome research, 2013

doi:10.1021/pr400098r.

Affinity profiling of the cellular kinome for the nucleotide cofactors ATP, ADP, and GTP.

Becher I, Savitski MM, Savitski MF, Hopf C, Bantscheff M, Drewes G

ACS chemical biology, 2013

doi:10.1021/cb3005879.

High-resolution enabled TMT 8-plexing.

Werner T, Becher I, Sweetman G, Doce C, Savitski MM, Bantscheff M

Analytical chemistry, 2012

doi:10.1021/ac301553x.

Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes.

Bantscheff M, Hopf C, Savitski MM, Dittmann A, Grandi P, Michon AM, Schlegl J, Abraham Y, Becher I, Bergamini G, Boesche M, Delling M, Dümpelfeld B, Eberhard D, Huthmacher C, Mathieson T, Poeckel D, Reader V, Strunk K, Sweetman G, Kruse U, Neubauer G, Ramsden NG, Drewes G

Nature biotechnology, 2011

doi:10.1038/nbt.1759.

H-score, a mass accuracy driven rescoring approach for improved peptide identification in modification rich samples.

Savitski MM, Mathieson T, Becher I, Bantscheff M

Journal of proteome research, 2010

doi:10.1021/pr1006813.