{"id":778,"date":"2024-03-26T23:55:12","date_gmt":"2024-03-26T23:55:12","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/?page_id=778"},"modified":"2024-06-22T17:28:58","modified_gmt":"2024-06-22T17:28:58","slug":"planexm","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/projects\/planexm\/","title":{"rendered":"PlanExM"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p class=\"vf-lede\">Revealing planktonic ultrastructural diversity with Expansion Microscopy.<\/p>\n<!--\/vf-lede-->\n\n\n\n\n<p><\/p>\n\n\n\n<p>The cellular physiology of microbial eukaryotic communities in the ocean remain largely unknown, as well as the impact of climate change on their ecology. In PlanExM we are coupling ultrastructure expansion microscopy (U-ExM) to fluorescence in situ hybridization (FISH) for species identification and the characterisation of cellular ultrastructure across mixed marine samples and key species drawn from TREC and the European culture collections. Plugging the gap between standard confocal imaging and EM techniques, we aim to create an atlas of native eukaryotic cellular biodiversity with unprecedented 3D spatial resolution, with a long-term view to assess the repercussions of climate change.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"575\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-content\/uploads\/2024\/05\/PlanExM_Dey_1.jpg\" alt=\"\" class=\"wp-image-1857\" srcset=\"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-content\/uploads\/2024\/05\/PlanExM_Dey_1.jpg 1024w, https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-content\/uploads\/2024\/05\/PlanExM_Dey_1-300x168.jpg 300w, https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-content\/uploads\/2024\/05\/PlanExM_Dey_1-768x431.jpg 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Diverse cell morphologies of Eutreptiella gymnastica post U-ExM and immunoflorescence. Tubulin (magenta), DNA (blue), Scale bar 10 microns. Image credit: Cl\u00e9mence Saint-Donat, Dey Group.<\/figcaption><\/figure>\n\n\n\n<div style=\"height:31px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Collaborators<\/h3>\n\n\n\n<p>Dudin Group, EPLF Lausanne<\/p>\n\n\n\n<p>Guichard and Hamel Group, University of Geneva<\/p>\n\n\n\n<p><a href=\"https:\/\/www.embl.org\/groups\/schwab\/\" data-type=\"link\" data-id=\"https:\/\/www.embl.org\/groups\/schwab\/\">Schwab team<\/a>, EMBL Heidelberg <\/p>\n\n\n\n<p><a href=\"https:\/\/www.embl.org\/groups\/saka\/\" data-type=\"link\" data-id=\"https:\/\/www.embl.org\/groups\/saka\/\">Saka group<\/a>, EMBL Heidelberg<\/p>\n\n\n\n<p class=\"vf-u-margin__bottom--800\"><\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Project lead<\/h3>\n\n\n<div class=\"vf-content-hub-html\" data-cache=\"3cb6beff\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-contains%5Bfield_person_full_name%5D=gautam%20dey&amp;filter-fields-empty=field_person_visible_internally&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bteam%2Corcid%5D=1&amp;limit=1&amp;pattern=vf-profile-inline -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"88934\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60034206.jpg?itok=hRHjXrUJ\" alt=\"image of Gautam Dey\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/gautam-dey\" class=\"vf-profile__link\">Gautam Dey<\/a>\n                  <\/h3>\n      \n              <p class=\"vf-profile__job-title\">\n          Group Leader\n        <\/p>\n      \n      \n      \n      \n      \n      \n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/88934\/88934\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":714,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-778","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/pages\/778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/comments?post=778"}],"version-history":[{"count":9,"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/pages\/778\/revisions"}],"predecessor-version":[{"id":2701,"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/pages\/778\/revisions\/2701"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/pages\/714"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/media?parent=778"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/planetary-biology\/wp-json\/wp\/v2\/embl_taxonomy?post=778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}