{"id":700,"date":"2023-10-18T10:36:55","date_gmt":"2023-10-18T10:36:55","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/?page_id=700"},"modified":"2023-11-03T23:51:48","modified_gmt":"2023-11-03T23:51:48","slug":"mitochondrial-thermal-proteome-profiling-for-the-study-of-oncometabolite-interactome","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/research\/mitochondrial-thermal-proteome-profiling-for-the-study-of-oncometabolite-interactome\/","title":{"rendered":"Mitochondrial thermal proteome Profiling for the study of oncometabolite interactome"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h2 class=\"wp-block-heading\">Background<\/h2>\n\n\n\n<p>Deregulation of mitochondrial metabolism is associated with several pathologies, including tumorigenesis. In particular, mutations in genes encoding tricarboxylic acid (TCA) cycle enzymes are present in a wide variety of human cancers. These mutations lead to the excessive accumulation of mitochondrial-derived metabolites that act as pro-oncogenic signaling molecules, the reason why they have been termed oncometabolites. Several mitochondrial and cytonuclear effects of oncometabolites have been described, including inhibition of mitochondrial respiration and \u03b1-ketoglutarate-dependent dioxygenases. However, these defects do not fully explain the pro-tumorigenic properties of oncometabolites, indicating additional mitochondrial or cellular proteome effects. Therefore, an unbiased interactome approach to studying oncometabolite-protein interactions within mitochondria and cancer cells is necessary to provide novel insights into the role of mitochondrial-derived metabolites in cancer.&nbsp;<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<h2 class=\"wp-block-heading\">Project<\/h2>\n\n\n\n<p>Our project aims to generate a new method to study mitochondrial-specific effects of oncometabolites by combining thermal proteome profiling (TPP, Savitski lab at EMBL) with rapid mitochondrial immunopurification (mito-IP, Abu-Remaileh lab at Stanford). Mitochondrial thermal proteome profiling (mito-TPP) will track direct targets of molecules and perturbations on the mitochondrial proteome. Therefore, mito-TPP will be a novel and powerful method to explore the effects of oncometabolites on mitochondria, providing new insights into their pro-oncogenic mechanisms and novel therapeutic targets to treat tumors harboring mutations in TCA cycle enzymes.&nbsp;<\/p>\n\n\n\n<p>This project is supported by a\u00a0<a href=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/activities\/fellowships\/\" data-type=\"page\" data-id=\"151\">Bridging Excellence Fellowship<\/a>, awarded to Pablo Rivera Mej\u00edas.\u00a0<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<h2 class=\"wp-block-heading\">Find out more:<\/h2>\n\n\n\n<p>Interested in finding out more about working at the interface of metabolism and organelle biology?&nbsp;<a href=\"mailto:stanford@embl.de\" target=\"_blank\" rel=\"noreferrer noopener\">Get in touch<\/a>, we would love to hear from you!<\/p>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">Collaborators:<\/h3>\n\n\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/abu-remaileh.png\" class=\"vf-profile__image\" alt=\"Headshot Monther Abu-Remaileh\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" >\n                    <a href=\"https:\/\/www.abu-remaileh.com\" target=\"_self\" class=\"vf-profile__link\">Abu-Remaileh Lab<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" >\n                Stanford            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"155\" src=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/savitski_misha.jpeg\" class=\"vf-profile__image\" alt=\"Headshot Misha Savitski\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/savitski_misha.jpeg 155w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/savitski_misha-150x150.jpeg 150w\" sizes=\"auto, (max-width: 155px) 100vw, 155px\" \/>\n    <h3 class=\"vf-profile__title\" >\n                    <a href=\"https:\/\/www.embl.org\/groups\/savitski\/\" target=\"_self\" class=\"vf-profile__link\">Savitski Team<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" >\n                EMBL            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"220\" height=\"220\" src=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/saka.jpg\" class=\"vf-profile__image\" alt=\"Headshot Sinem Saka\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/saka.jpg 220w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/saka-150x150.jpg 150w\" sizes=\"auto, (max-width: 220px) 100vw, 220px\" \/>\n    <h3 class=\"vf-profile__title\" >\n                    <a href=\"https:\/\/www.embl.org\/groups\/saka\/\" target=\"_self\" class=\"vf-profile__link\">Saka Group<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" >\n                EMBL            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<figure class=\"vf-figure wp-block-image  | vf-figure--align vf-figure--align-inline-start   size-medium is-resized\"><img loading=\"lazy\" decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/BridgingExcellenceLogo_BLACK-300x134.png\" alt=\"Logo Bridging Excellence Fellowships black\" class=\"wp-image-646\" width=\"225\" height=\"101\" srcset=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/BridgingExcellenceLogo_BLACK-300x134.png 300w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/BridgingExcellenceLogo_BLACK-1024x456.png 1024w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/BridgingExcellenceLogo_BLACK-768x342.png 768w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/BridgingExcellenceLogo_BLACK.png 1064w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/figure>\n\n\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-300x300.jpg\" class=\"vf-profile__image\" alt=\"Headshot ablo Rivera Mejias\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-300x300.jpg 300w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-1024x1024.jpg 1024w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-150x150.jpg 150w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-768x768.jpg 768w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-1536x1536.jpg 1536w, https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-content\/uploads\/2023\/10\/RiveraMejias_Pablo_220x-2048x2048.jpg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\n    <h3 class=\"vf-profile__title\" >\n                    Pablo Rivera Mejias            <\/h3>\n    \n                <p class=\"vf-profile__text\" >\n                Bridging Excellence Fellow            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":7,"featured_media":0,"parent":128,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-700","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/pages\/700","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/comments?post=700"}],"version-history":[{"count":21,"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/pages\/700\/revisions"}],"predecessor-version":[{"id":1677,"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/pages\/700\/revisions\/1677"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/pages\/128"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/media?parent=700"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/life-science-alliance\/wp-json\/wp\/v2\/embl_taxonomy?post=700"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}