{"id":515,"date":"2024-03-26T12:20:44","date_gmt":"2024-03-26T12:20:44","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/infection-biology\/?page_id=515"},"modified":"2024-10-04T03:51:18","modified_gmt":"2024-10-04T03:51:18","slug":"understanding-the-link-between-the-antiviral-activity-rna-binding-and-e3-ligase-activity-of-trim25","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/infection-biology\/projects\/understanding-the-link-between-the-antiviral-activity-rna-binding-and-e3-ligase-activity-of-trim25\/","title":{"rendered":"Understanding the link between the antiviral activity, RNA binding, and E3 ligase activity of TRIM25"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<h3 class=\"wp-block-heading\">TRIM25, an RNA-binding E3-ubiquitin ligase, plays a crucial role in the innate immune response against RNA viruses, yet the specifics of its mechanism remain unclear. <\/h3>\n\n\n\n<p>Preliminary data suggest that TRIM25&#8217;s impaired functions reduce antiviral activity, but the molecular details are unknown. This project aims to dissect the relationship between TRIM25&#8217;s RNA-binding, its E3-ligase activity, and antiviral efficacy. Employing a comprehensive approach, including protein proximity assays, genome-wide RNA binding analysis, and structural biology, the study seeks to identify TRIM25\u2019s ubiquitylation and RNA targets before and after viral infection. Additionally, experiments with various RNA viruses will determine if TRIM25&#8217;s defence mechanism is specific to Sindbis virus or applicable across different viral families. This collaborative effort will advance our understanding of TRIM25&#8217;s role in infection biology.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Janosch Hennig<\/strong> (EMBL), <strong>Mikhail Savitski<\/strong> (EMBL), <strong>Sagar Bhogaraju<\/strong> (EMBL), <strong>Alfredo Castello Palomares<\/strong> (CVR Glasgow)<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p class=\"vf-text--body vf-text-body--3\"><a href=\"https:\/\/doi.org\/10.1016\/j.molcel.2024.08.027\">Exploring the expanding universe of host-virus interactions mediated by viral RNA<\/a><br><em>Castello A, \u00c1lvarez L, Kamel W, Iselin L, Hennig J<\/em><br>Molecular Cell, Volume 84, Issue 19, 3706 \u2013 3721 (2024)<br>doi:10.1016\/j.molcel.2024.08.027<\/p>\n\n\n\n<p class=\"vf-text--body vf-text-body--3\"><a href=\"https:\/\/doi.org\/10.1038\/s41467-024-52918-x\">The molecular dissection of TRIM25\u2019s RNA-binding mechanism provides key insights into its antiviral activity<\/a><br><em>\u00c1lvarez L, [&#8230;], Castello A, Hennig J<\/em><br>Nature Communications, Volume 15, Article number: 8485 (2024)<br>doi:10.1038\/s41467-024-52918-x<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"580\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-content\/uploads\/2024\/02\/TRIM25_IBTT-image-1024x580.png\" alt=\"\" class=\"wp-image-407\" srcset=\"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-content\/uploads\/2024\/02\/TRIM25_IBTT-image-1024x580.png 1024w, https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-content\/uploads\/2024\/02\/TRIM25_IBTT-image-300x170.png 300w, https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-content\/uploads\/2024\/02\/TRIM25_IBTT-image-768x435.png 768w, https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-content\/uploads\/2024\/02\/TRIM25_IBTT-image.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":155,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-515","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/pages\/515","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/comments?post=515"}],"version-history":[{"count":2,"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/pages\/515\/revisions"}],"predecessor-version":[{"id":7653,"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/pages\/515\/revisions\/7653"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/pages\/155"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/media?parent=515"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/infection-biology\/wp-json\/wp\/v2\/embl_taxonomy?post=515"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}