{"id":59553,"date":"2025-05-30T12:37:28","date_gmt":"2025-05-30T12:37:28","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?p=59553"},"modified":"2025-05-30T12:37:28","modified_gmt":"2025-05-30T12:37:28","slug":"join-the-basics-of-cryo-fib-milling-and-in-situ-cryo-electron-tomography-course-13-17-october-2025","status":"publish","type":"post","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/blog\/2025\/05\/join-the-basics-of-cryo-fib-milling-and-in-situ-cryo-electron-tomography-course-13-17-october-2025\/","title":{"rendered":"Join the Basics of cryo-FIB milling and in situ cryo-electron tomography course (13\u201317 October 2025)"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\"><\/h3>\n\n\n\n<p>The&nbsp;<strong>EMBL Imaging Centre<\/strong>&nbsp;invites applications for an immersive&nbsp;<strong>5-day hands-on course<\/strong>&nbsp;focused on the&nbsp;<strong>cryo-lamella tomography workflow<\/strong>&nbsp;\u2014 a cutting-edge technique to study cellular samples in their native environment at high resolution.<\/p>\n\n\n\n<p>\ud83d\udcc5&nbsp;<strong>Course Dates:<\/strong>&nbsp;13 \u2013 17 October 2025<br>\ud83d\udccd&nbsp;<strong>Location:<\/strong>&nbsp;EMBL Imaging Centre, Heidelberg, Germany<br>\ud83d\udc65&nbsp;<strong>Participants:<\/strong>&nbsp;Limited to 8<br>\ud83d\udcdd&nbsp;<strong>Registration Deadline:<\/strong>&nbsp;15 July 2025<br>\ud83d\udcac&nbsp;<strong>Announcement of Participants:<\/strong>&nbsp;25 July 2025<br>\ud83d\udcb0&nbsp;<strong>Registration Fee:<\/strong>&nbsp;Free (travel and accommodation must be covered by participants)<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\ud83d\udd0d\u00a0<strong>Course highlights<\/strong><\/h3>\n\n\n\n<p>This course will provide\u00a0<strong>theoretical foundations<\/strong>\u00a0and\u00a0<strong>practical training<\/strong>\u00a0in:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Sample vitrification<\/strong>: Plunge- and high-pressure freezing techniques<\/li>\n\n\n\n<li><strong>Cryo-FIB milling<\/strong>: On-grid lamella preparation using state-of-the-art instruments<\/li>\n\n\n\n<li><strong>Cryo-electron tomography (cryo-ET)<\/strong>: Data acquisition and high-resolution imaging<\/li>\n\n\n\n<li><strong>Computational analysis<\/strong>: Tomogram reconstruction, segmentation, and sub-tomogram averaging<\/li>\n<\/ul>\n\n\n\n<p>An&nbsp;<strong>open equipment day<\/strong>&nbsp;will offer further exploration opportunities with expert guidance.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\ud83c\udf93\u00a0<strong>Lectures from leading scientists<\/strong><\/h3>\n\n\n\n<p>Featuring invited talks by:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Dr. Petr Chlanda<\/strong>\u00a0\u2013 Cryo-CLEM studies on Ebola virus<\/li>\n\n\n\n<li><strong>Dr. Bettina Zens<\/strong>\u00a0\u2013 Cryo-lift-out and ECM analysis<\/li>\n<\/ul>\n\n\n\n<p>And lectures by EMBL IC experts on sample prep, cryo-FIB milling, tomography tools, 3D modeling, and molecular-level analyses.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\ud83d\udee0\ufe0f&nbsp;<strong>Hands-On Practicals<\/strong><\/h3>\n\n\n\n<p>You will gain hands-on experience with world-class instruments:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>GP2 &amp; EM ICE (Leica Microsystems)<\/strong>\u00a0\u2013 Sample vitrification<\/li>\n\n\n\n<li><strong>Aquilos 2 (ThermoFisher Scientific)<\/strong>\u00a0\u2013 Automated cryo-FIB milling<\/li>\n\n\n\n<li><strong>Titan Krios G4 (ThermoFisher Scientific)<\/strong>\u00a0\u2013 High-resolution tomography<\/li>\n\n\n\n<li><strong>AreTomo3, MIB, SerialEM<\/strong>\u00a0\u2013 Computational tools for 3D reconstruction and segmentation<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">\ud83d\udcdd&nbsp;<strong>How to Apply<\/strong><\/h3>\n\n\n\n<p>Interested participants must submit:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>A\u00a0<strong>CV<\/strong><\/li>\n\n\n\n<li>A\u00a0<strong>motivation letter<\/strong>\u00a0describing your background, access to cryo-lamella instrumentation, and how this course aligns with your research goals<\/li>\n<\/ul>\n\n\n\n<p>\ud83d\udce9&nbsp;<strong>Apply now!<\/strong>&nbsp;Space is limited and highly competitive.<\/p>\n\n\n\n<p>\ud83d\udc49\u00a0<strong><a href=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/basicsfibcourse2025\/\">https:\/\/www.embl.org\/about\/info\/imaging-centre\/basicsfibcourse2025\/<\/a><\/strong><\/p>\n\n\n\n<p>For more information, contact:<br><strong>Georg Wolff<\/strong>&nbsp;\u2013&nbsp;<a>georg.wolff@embl.de<\/a><br><strong>Anna Steyer<\/strong>&nbsp;\u2013&nbsp;<a>anna.steyer@embl.de<\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Don&#8217;t miss this opportunity to master cryo-FIB milling and in situ cryo-electron tomography!<\/h3>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The&nbsp;EMBL Imaging Centre&nbsp;invites applications for an immersive&nbsp;5-day hands-on course&nbsp;focused on the&nbsp;cryo-lamella tomography workflow&nbsp;\u2014 a cutting-edge technique to study cellular samples in their native environment at high resolution. \ud83d\udcc5&nbsp;Course Dates:&nbsp;13&hellip;<\/p>\n","protected":false},"author":16,"featured_media":59555,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"embl_taxonomy":[],"class_list":["post-59553","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general"],"acf":[],"embl_taxonomy_terms":[],"featured_image_src":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2025\/05\/EMBL-IC-homepage-photo-size.jpg","_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/posts\/59553","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=59553"}],"version-history":[{"count":1,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/posts\/59553\/revisions"}],"predecessor-version":[{"id":59557,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/posts\/59553\/revisions\/59557"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media\/59555"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=59553"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/categories?post=59553"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/tags?post=59553"},{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=59553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}