{"id":76563,"date":"2026-04-09T07:45:34","date_gmt":"2026-04-09T07:45:34","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=76563"},"modified":"2026-04-15T08:35:31","modified_gmt":"2026-04-15T08:35:31","slug":"abberior-minflux-workshop-minflux-imaging-and-tracking-on-the-nanoscale","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/abberior-minflux-workshop-minflux-imaging-and-tracking-on-the-nanoscale\/","title":{"rendered":"Abberior MINFLUX Workshop: MINFLUX Imaging and Tracking on the Nanoscale"},"content":{"rendered":"\n

Nickels Jensen<\/strong>, Abberior Instruments GmbH<\/a>, Hans-Adolf-Krebs-Weg 6, 37077 G\u00f6ttingen<\/p>\n\n\n\n

<\/p>\n\n\n\n

Understanding the structure and dynamics of biological systems at the molecular scale is essential for revealing the principles of cellular function. Imaging and tracking are therefore two of the most important approaches for studying biological systems: imaging reveals molecular architecture, while tracking captures dynamic processes, often occurring on rapid timescales. To address both, microscopy methods must combine molecular specificity with nanometer-scale spatial resolution and high temporal precision.<\/p>\n\n\n\n

MINFLUX nanoscopy meets this challenge by enabling both the precise imaging of biological structures and the tracking of molecular dynamics with exceptional accuracy [1]. Through iterative localization, MINFLUX determines fluorophore positions with exceptional precision while maximizing the information gained from each detected photon, making it the most photon-efficient approach in super-resolution microscopy. This enables isotropic 3D localization precisions of a few nanometers and temporal resolutions down to 100 \u00b5s in tracking experiments [Fig.1].<\/p>\n\n\n\n

Here, we present the MINFLUX microscope, implemented on a conventional fluorescence microscope stand to bring this powerful technology into standard experimental workflows and make it more broadly accessible for biological research [2].<\/p>\n\n\n\n

Combining ultra-high localization precision with user-friendly operation, the system enables a wider community of researchers to apply MINFLUX to biological questions. In line with Abberior\u2019s vision of science beyond barriers, this workshop offers participants the opportunity to gain deeper insight into the capabilities of MINFLUX and to explore how it is helping to broaden access to nanoscale imaging and tracking.<\/p>\n\n\n\n

In addition to the symposium programme, participants are warmly invited to schedule a demonstration of MINFLUX. <\/p>\n\n\n\n

\"\"
Fig 1. Isotropic 3D Image of a Nuclear Pore (Nup96-AlexaFluor647) [A] and Tracking of Atto 647N-coupled lipid in a bilayer [B].<\/figcaption><\/figure>\n\n\n\n

References:<\/strong>
[1] Balzarotti, F., et al. : Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science. 2017 Feb 10;355(6325):606-612.<\/a><\/strong><\/p>\n\n\n\n

[2] Schmidt, R., et al.: MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat Commun. 2021 Mar 5;12(1):1478.<\/a>
<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Nickels Jensen, Abberior Instruments GmbH, Hans-Adolf-Krebs-Weg 6, 37077 G\u00f6ttingen Understanding the structure and dynamics of biological systems at the molecular scale is essential for revealing the principles of cellular function. Imaging and tracking are therefore two of the most important…<\/p>\n","protected":false},"author":16,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-76563","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/76563","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=76563"}],"version-history":[{"count":3,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/76563\/revisions"}],"predecessor-version":[{"id":76957,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/76563\/revisions\/76957"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=76563"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=76563"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}