{"id":44879,"date":"2024-08-15T04:56:22","date_gmt":"2024-08-15T04:56:22","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=44879"},"modified":"2026-01-29T12:35:39","modified_gmt":"2026-01-29T12:35:39","slug":"intravital-and-tissue-imaging","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/intravital-and-tissue-imaging\/","title":{"rendered":"Intravital and Tissue imaging"},"content":{"rendered":"<style>\n      #wp-block-1 .vf-card-container::before {\n  background:url(https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/02\/Screenshot-2022-02-11-at-10.41.00.png);\n  background-position: 50%;\n  background-size: cover; }\n <\/style>\n\n<section id=\"wp-block-1\">\n  <div class=\"vf-card-container vf-card-container__col-3 | vf-u-fullbleed  \n\">\n        <div class=\"vf-card-container__inner\">\n            <div class=\"vf-section-header | vf-u-margin__bottom--600\">\n        <h2 class=\"vf-section-header__heading\" >\n        Services offered    <\/h2>\n                <p class=\"vf-section-header__text\">For organismal and intravital imaging we offer light-sheet, multi-photon, and Brillouin microscopy modalities, enabling longitudinal imaging studies and quantitative mapping of mechanical properties deep within living organisms. Imaging of tissue sections is additionally supported on widefield and microdissection microscopes.<\/p>\n              <\/div>\n      \n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nLearn more about light-sheet microscopy<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>Selective plane illumination microscopy (SPIM) or light-sheet microscopy is characterised by orthogonal illumination with respect to detection and is in particular <strong>suited for gentle long-term imaging by optical sectioning of thick living specimens<\/strong>.&nbsp;<\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nLearn more about multi-photon microscopy<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>Nonlinear microscopy methods like multiphoton fluorescence microscopy and also a range of label-free imaging methods use local nonlinear interactions between the excitation light and the sample matter to create highly specific signals that allow a better understanding of thick and complex samples, both alive and fixed.<\/p>\n\n\n\n<p>In two-photon microscopy a much deeper penetration of the sample is achieved for imaging by the use of less scattering excitation light in the near infrared range and by the use of non-descanned detection for the emission light that is returning from the sample. Since the laser excitation is pulsed to achieve high local photon densities for nonlinear effects while keeping the total energy dosage low, the method can be efficiently combined with fluorescence lifetime imaging for additional information and signal separation. The use of longer wavelengths for the multiphoton effect makes this technique <strong>very suitable for in-vivo imaging in thick and complex samples and for intravital imaging<\/strong>.<\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nLearn more about Brillouin microscopy<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>Brillouin microscopy is a non-contact, label-free optical technique that <strong>maps the mechanical properties of materials and biological tissues at microscopic resolution<\/strong>. By analysing the interaction between light and naturally occurring acoustic waves within a sample, it provides quantitative insight into stiffness and viscoelasticity without physical perturbation. This makes Brillouin microscopy a powerful tool for studying biomechanics, disease progression, and material behavior in living systems and soft matter.<\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nLearn more about laser microdissection<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>Regions of interest (ROI) can be isolated from entire areas of tissue, like cell clusters or tumours, down to single cells or even subcellular structures, such as chromosomes. This live cell dissection <strong>enables downstream analysis via omics technologies or re-cultivation<\/strong>.<\/p>\n\n<\/div>\n<\/details>\n\n\n          <\/div>\n      <\/div>\n<\/section>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-1\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-0a185209-0a3b-4b7e-a5ae-157aae031d04\" data-vf-js-location-nearest-activation-target=\"\">Brillouin Microscope<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-e78cc4ec-b849-4550-8d08-3065843edc69\" data-vf-js-location-nearest-activation-target=\"\">STELLARIS 8 DIVE Falcon<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-9e031da5-4d51-4e8a-a613-8eb064023626\" data-vf-js-location-nearest-activation-target=\"\">LMD7<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-db56f577-aa08-45a6-b619-e6afd8fcfb0e\" data-vf-js-location-nearest-activation-target=\"\">MultiView SPIM<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-508e7352-7859-4e14-9fad-f5722a47e965\" data-vf-js-location-nearest-activation-target=\"\">THUNDER Imager 3D Tissue<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-0a185209-0a3b-4b7e-a5ae-157aae031d04\"><h2>Brillouin Microscope<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p><strong>Brillouin microscopy<\/strong> is a label-free form of imaging that allows to measure a sample&#8217;s mechanical properties. Molecular vibrations inside a sample generate acoustic waves (phonons) whose velocity depends on the stiffness and viscosity of the material. Phonons can interact with photons in the sample and exchange energy, resulting in a slight frequency shift of the light wave. This phenomenon is called Brillouin scattering. By measuring the frequency shift, one can estimate the stiffness and viscosity of the materials at the position where a laser beam is focused.&nbsp;<\/p>\n\n\n\n<p>The confocal Brillouin microscope in Imaging Centre is based on Brillouin microscopy developments in <strong><a href=\"https:\/\/www.embl.org\/groups\/prevedel\/\" data-type=\"link\" data-id=\"https:\/\/www.embl.org\/groups\/prevedel\/\">Robert Prevedel\u2019s group<\/a><\/strong> at EMBL Heidelberg. The IC\u2019s Brillouin microscope is combined with a fully equipped confocal fluorescence microscope for multimodal imaging.&nbsp;&nbsp;&nbsp;<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>More stable and fast scanning mechanism: a beam-steering scanning mechanism for Brillouin imaging instead of stage scanning.<\/li>\n\n\n\n<li>More&nbsp;precise but simpler calibration for frequency shift: electro-optical modulation for reference measurement<\/li>\n\n\n\n<li>Further suppression of Rayleigh scattering: an additional 60 dB suppression by Birefringence-Induced phase delay (BIPD) filter<\/li>\n\n\n\n<li>High end confocal fluorescence microscope: Commercially available module with fully integrated software<\/li>\n\n\n\n<li>Hyperspectral and multiplexing imaging: An array detector with a spectrometer to measure the spectra of fluorescence<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<p>Brillouin imaging<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Probing wavelength: 660 nm<\/li>\n\n\n\n<li>40X magnification<\/li>\n\n\n\n<li>Pixel dwell time: 50-100&nbsp;ms<\/li>\n\n\n\n<li>Frequency shift precision : 20 MHz<\/li>\n<\/ul>\n\n\n\n<p>Confocal fluorescence imaging<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Objective: 40X\/1.2 water immersion, 40X\/1.3 oil&nbsp;immersion<\/li>\n\n\n\n<li>Laser: 405\/488\/561\/633 nm<\/li>\n\n\n\n<li>Quasar detector for hyperspectral imaging&nbsp;<\/li>\n<\/ul>\n\n\n\n<p><strong>References<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Prevedel, R., Diz-Mu\u00f1oz, A., Ruocco, G. &amp; Antonacci, G. Brillouin microscopy: an emerging tool for mechanobiology. Nat. Methods 16, 969\u2013977 (2019).&nbsp;<\/li>\n\n\n\n<li>Zhang, J. &amp; Scarcelli, G. Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes. Nat. Protoc. 16, 1251\u20131275 (2021).&nbsp;<\/li>\n\n\n\n<li>Antonacci, G. et al. Birefringence-induced phase delay enables Brillouin mechanical imaging in turbid media. Nat. Commun. 15, 5202 (2024).&nbsp;<\/li>\n\n\n\n<li>Testi, C. et al. Electro-Optic Modulator source as sample-free calibrator and frequency stabilizer for Brillouin Microscopy. Preprint at https:\/\/doi.org\/10.48550\/arXiv.2412.20516 (2025).<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"726\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Slide1-1024x726.jpeg\" alt=\"\" class=\"wp-image-72159\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Slide1-1024x726.jpeg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Slide1-300x213.jpeg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Slide1-768x544.jpeg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Slide1-1536x1088.jpeg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Slide1.jpeg 1767w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Brillouin image of HeLa cells, where image intensity represents the frequency shift arising from Brillouin scattering. This frequency shift is used to evaluate the real part of the longitudinal modulus, providing a measure of local cellular stiffness. Credit: Tzu-Lun Wang\/EMBL<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/EM-IC-7484-1024x683.jpg\" alt=\"\" class=\"wp-image-71303\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/EM-IC-7484-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/EM-IC-7484-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/EM-IC-7484-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/EM-IC-7484-1536x1025.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/EM-IC-7484-2048x1366.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Brillouin Microscope. Credit: Joseph Franciosa\/EMBL <\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-e78cc4ec-b849-4550-8d08-3065843edc69\"><h2>STELLARIS 8 DIVE Falcon<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>STELLARIS 8 DIVE with the 4Tune detection system is a <strong>spectrally tunable multi-photon microscope<\/strong> from Leica Microsystems. Four non-descanned spectrally flexible channels provide multicolour multiphoton imaging at &gt; 1 mm depth.&nbsp;<\/p>\n\n\n\n<p>Lifetime-based information can be directly obtained in addition to distinguish spectrally similar fluorophores, separate second harmonic (SHG) signals from fluorescence or visualise the metabolic state of cells. Lifetime analysis and quantification can be done FALCON.&nbsp;<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong>:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>4 spectrally tunable NDD detectors in the visible range (380 \u2013 800 nm)<\/li>\n\n\n\n<li>Qualitative and semi-quantitative lifetime-based information with&nbsp;TauSense<\/li>\n\n\n\n<li>Fully quantitative FLIM analysis with FALCON, including phasors&nbsp;<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong>:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Laser lines: IR: tunable 680 \u2013 1080 nm and 680 \u2013 1300 nm, fixed 1040 nm; Confocal: White Light Lasers (WLL) 440 \u2013 790 nm, 405 nm, and 488 nm<\/li>\n\n\n\n<li>Four NDD channels equipped with Power&nbsp;HyD&nbsp;X (tunable from 380 \u2013 800 nm), five spectrally tunable internal counting detectors (3&nbsp;HyD&nbsp;S, 1&nbsp;HyD&nbsp;X, and 1&nbsp;HyD&nbsp;R)<\/li>\n\n\n\n<li>Upright confocal fixed-stage (DM6 CFS) stand furnished with&nbsp;Scientifica&nbsp;scanning stage<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"805\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/IC-Microscope-Leica_DIVE-1024x805.jpg\" alt=\"\" class=\"wp-image-23644\" style=\"object-fit:cover\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/IC-Microscope-Leica_DIVE-1024x805.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/IC-Microscope-Leica_DIVE-300x236.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/IC-Microscope-Leica_DIVE-768x604.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/IC-Microscope-Leica_DIVE-1536x1208.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/IC-Microscope-Leica_DIVE-2048x1610.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">STELLARIS 8 DIVE Falcon. Credit: Stuart Bailey\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-9e031da5-4d51-4e8a-a613-8eb064023626\"><h2>LMD7<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The LMD7 <strong>laser microdissection microscope<\/strong> from Leica Microsystems enables users to isolate specific microscopic target areas under visual control for downstream molecular biology analysis. Regions of interest (ROI) can be isolated from entire areas of tissue, like cell clusters or tumours, down to single cells or even subcellular structures, such as chromosomes. Live cell dissection for downstream analysis or re-cultivation (cloning) as well as laser manipulation is possible. The LMD7 is equipped with a LMT350 scanning stage which allows collection of&nbsp;dissectates&nbsp;directly into regular PCR tubes or multi-well plates, such as 96-well PCR plates. Downstream analysis is typically used for genomics (DNA), transcriptomics (mRNA, miRNA) by qPCR, Microarray, RNA-seq, next generation sequencing (NGS), proteomics, metabolomics done with Western blots, and mass spectrometry.&nbsp;Lipidomics&nbsp;can be done with laser microdissection.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Laser movement by optics<\/li>\n\n\n\n<li>Direct collection into 96-well plates simply by gravity<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Wavelength: 349 nm<\/li>\n\n\n\n<li>Pulse frequency: 10\u20135,000 Hz<\/li>\n\n\n\n<li>Pulse length: &lt;4 ns<\/li>\n\n\n\n<li>Average pulse energy: 120&nbsp;\u03bcJ<\/li>\n\n\n\n<li>Range of dedicated LMD objectives: 2.5x, 5x, 10x, 20x, 40x, 63x, and 150x<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/08\/LMD7_Application_1440x1080_2-1024x768.jpg\" alt=\"Credit: Falk Schlaudraff\/Leica Microsystems\" class=\"wp-image-2460\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/08\/LMD7_Application_1440x1080_2-1024x768.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/08\/LMD7_Application_1440x1080_2-300x225.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/08\/LMD7_Application_1440x1080_2-768x576.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/08\/LMD7_Application_1440x1080_2.jpg 1440w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Liver (pig): 16 \u00b5m cryo-section stained with cresyl violet. Image series of the laser excision and collection of a tissue subregion. Image taken with Leica LMD7. Credit: Falk Schlaudraff\/Leica Microsystems<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"583\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_123-Edit-1024x583.jpg\" alt=\"Credit: Stuart Bailey\/EMBL\" class=\"wp-image-4536\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_123-Edit-1024x583.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_123-Edit-300x171.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_123-Edit-768x437.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_123-Edit.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Leica LMD7. Credit: Stuart Bailey\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-db56f577-aa08-45a6-b619-e6afd8fcfb0e\"><h2>MultiView SPIM<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The MultiView-SPIM or<strong>MuVi-SPIM<\/strong> (selective plane illumination microscope) is an advanced <strong>light-sheet fluorescence microscope<\/strong> utilising multiple orthogonal optical paths for in vivo imaging of small embryos, organisms and cell cultures. The MuVi-SPIM available at the EMBL IC has originally been developed by the lab of Lars Hufnagel at EMBL and further developed by the lab of Robert Prevedel at EMBL. Owing to its plane wise illumination confinement and fast imaging on multiple cameras, the MultiView-SPIM is able to capture developmental dynamics with minimal photodamage to the living subjects.<\/p>\n\n\n\n<a href=\"https:\/\/doi.org\/10.1038\/ncomms9881\" target=\"\">\n    <button class=\"vf-button vf-button--link vf-button--sm\">\n        Publication describing the confocal MuVi-SPIM    <\/button>\n<\/a>\n<!--\/vf-button-->\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Multi-view reconstruction via fusion of multiple camera views<\/li>\n\n\n\n<li>Environmental control (Temperatures, CO2 etc.)<\/li>\n\n\n\n<li>Variable magnification 22 \u2013 33x (600 \u2013 400 um field of view)<\/li>\n\n\n\n<li>Sub-micron resolution for superficial imaging, performance at depth dependent on sample optical properties.<\/li>\n\n\n\n<li>Imaging typically at 50 \u2013 100 frames per second, volume rate typically 0.1 \u2013 1 volume per second<\/li>\n\n\n\n<li>Multi-color imaging achieved sequentially with fast filter wheels<\/li>\n\n\n\n<li>Live samples can be imaged over several days<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Light sheets available at 488, 515, 594 nm (and 685, 808 nm on request)<\/li>\n\n\n\n<li>Light sheets generated by beam scanning and combined with camera-based confocal line detection to reject blur and enhance contrast<\/li>\n\n\n\n<li>Motorised x,y,z and rotation stages<\/li>\n\n\n\n<li>Sample mounting in refractive index matching tubes (FEP n = 1.33) or extruded in gelated column from glass capillaries<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"326\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/dimitri_kromm-take_a_breath-1024x326.jpg\" alt=\"Credit: Dimitri Kromm\/EMBL\" class=\"wp-image-15042\" style=\"object-fit:cover\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/dimitri_kromm-take_a_breath-1024x326.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/dimitri_kromm-take_a_breath-300x95.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/dimitri_kromm-take_a_breath-768x244.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/dimitri_kromm-take_a_breath-1536x489.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/dimitri_kromm-take_a_breath.jpg 1678w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Projection image of the tracheal system of a&nbsp;<em>Drosophila melanogaster<\/em>&nbsp;larva. Credit: Dimitri Kromm\/EMBL.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/Dimitri-Kromm-Oryzias-latipes-from-head-to-fin-1024x576.jpg\" alt=\"credit: Dimitri Kromm, EMBL\" class=\"wp-image-15044\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/Dimitri-Kromm-Oryzias-latipes-from-head-to-fin-1024x576.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/Dimitri-Kromm-Oryzias-latipes-from-head-to-fin-300x169.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/Dimitri-Kromm-Oryzias-latipes-from-head-to-fin-768x432.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/Dimitri-Kromm-Oryzias-latipes-from-head-to-fin.jpg 1167w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Projection image of a&nbsp;<em>Oryzias latipes<\/em>&nbsp;fish larva. Credit: Dimitri Kromm\/EMBL.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/MuVi-SPIM-1024x683.jpg\" alt=\"\" class=\"wp-image-23648\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/MuVi-SPIM-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/MuVi-SPIM-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/MuVi-SPIM-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/MuVi-SPIM-1536x1025.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/MuVi-SPIM-2048x1366.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">MultiView SPIM. Credit: Stuart Bailey\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-508e7352-7859-4e14-9fad-f5722a47e965\"><h2>THUNDER Imager 3D Tissue<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The THUNDER Imager Tissue from Leica Microsystems allows real-time fluorescence imaging of 3D tissue sections typically used for neuroscience and histology research. It also enables the acquisition of rich, detailed images of thick tissues. These images are free of haze from out-of-focus blur because of Computational&nbsp;Clearing for&nbsp;haze removal. Structures like axons and dendrites of neurons in a brain slice can be imaged.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Rapidly acquisition of blur-free images even deep within thick sections<\/li>\n\n\n\n<li>Get fast overviews of whole tissue sections<\/li>\n\n\n\n<li>Imaging and analysis workflow within the LAS X operation software<\/li>\n\n\n\n<li>Processing and reconstruction of complex images with the&nbsp;Aivia&nbsp;software platform<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Based on a fully automated upright research microscope for the acquisition of multi-color 3D images<\/li>\n\n\n\n<li>sCMOS&nbsp;camera system<\/li>\n\n\n\n<li>Software creates blur-free large overviews of the entire tissue specimen<\/li>\n\n\n\n<li>Precise motorised z-focus drive to capture images in the z-direction and visualise them with the 3D Viewer<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"630\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Thunder-3D-multiscale-2-1-1024x630.jpg\" alt=\"Credit: Falco Krueger\/Leica Microsystems\" class=\"wp-image-4652\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Thunder-3D-multiscale-2-1-1024x630.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Thunder-3D-multiscale-2-1-300x184.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Thunder-3D-multiscale-2-1-768x472.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Thunder-3D-multiscale-2-1.jpg 1132w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Mouse kidney: Nuclei (blue), glomeruli (green), actin (red). Tilescanned overview of the whole kidney section. Image taken with Leica Thunder 3D Tissue. Credit: Falco Krueger\/Leica Microsystems.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"747\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/THUNDER-Imager-3D-Tissue-1024x747.jpg\" alt=\"\" class=\"wp-image-23654\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/THUNDER-Imager-3D-Tissue-1024x747.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/THUNDER-Imager-3D-Tissue-300x219.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/THUNDER-Imager-3D-Tissue-768x560.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/THUNDER-Imager-3D-Tissue-1536x1120.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2023\/05\/THUNDER-Imager-3D-Tissue-2048x1494.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">THUNDER Imager 3D Tissue. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":10,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-44879","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/44879","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=44879"}],"version-history":[{"count":20,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/44879\/revisions"}],"predecessor-version":[{"id":72391,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/44879\/revisions\/72391"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=44879"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=44879"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}