{"id":44827,"date":"2024-08-15T04:55:24","date_gmt":"2024-08-15T04:55:24","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=44827"},"modified":"2026-01-29T12:36:09","modified_gmt":"2026-01-29T12:36:09","slug":"live-cell-imaging","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/live-cell-imaging\/","title":{"rendered":"Live-cell imaging"},"content":{"rendered":"<style>\n      #wp-block-1 .vf-card-container::before {\n  background:url(https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/02\/Screenshot-2022-02-11-at-10.41.00.png);\n  background-position: 50%;\n  background-size: cover; }\n <\/style>\n\n<section id=\"wp-block-1\">\n  <div class=\"vf-card-container vf-card-container__col-3 | vf-u-fullbleed  \n\">\n        <div class=\"vf-card-container__inner\">\n            <div class=\"vf-section-header | vf-u-margin__bottom--600\">\n        <h2 class=\"vf-section-header__heading\" >\n        Services offered    <\/h2>\n                <p class=\"vf-section-header__text\">For live-cell and time-lapse imaging we offer a wide range of technologies crossing the scales of biology including confocal, light-sheet and widefield microscopes.<\/p>\n              <\/div>\n      \n\n<p><\/p>\n\n\n          <\/div>\n      <\/div>\n<\/section>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-1\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-81d31ac1-ceb6-4531-ab61-322b229b4f4f\" data-vf-js-location-nearest-activation-target=\"\">Oblique Plain Microscope (OPM)<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-55ca62bd-2f7d-48c2-868e-5b89a7dd7512\" data-vf-js-location-nearest-activation-target=\"\">STELLARIS 8 STED Falcon<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-f7c3be18-6d8a-4021-a00d-1a8382e3163c\" data-vf-js-location-nearest-activation-target=\"\">MICA<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-88cadd0c-4e86-4adb-9d68-f766baf1354b\" data-vf-js-location-nearest-activation-target=\"\">THUNDER Imager Live Cell<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-297bd44f-71d5-4404-aada-ab6edf34f3c4\" data-vf-js-location-nearest-activation-target=\"\">DMi8 S with TIRF module<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-81d31ac1-ceb6-4531-ab61-322b229b4f4f\"><h2>Oblique Plain Microscope (OPM)<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>Oblique Plane Microscopy is a fast and gentle technique for volumetric imaging of living samples (Dunsby, Optics Express, 2008) and belongs to the family of <strong>light-sheet fluorescence microscopy<\/strong> techniques, such as the archetypal SPIM (Huisken et al., Science, 2004) and MuVi-SPIM (Krzic et al., Nature Methods, 2012) developed at EMBL. Light sheet techniques are fast as they capture 3D volumes in a plane-wise manner and gentle since laser illumination is confined purely within the plane.<\/p>\n\n\n\n<p>While light sheet microscopes generally use separate objectives for illumination and imaging and have specific requirements for sample mounting, OPM uses a single objective lens to illuminate an obliquely oriented plane and collect the emitted fluorescence.&nbsp; Light-sheet imaging can therefore be performed on an inverted microscope for samples on standard coverslips\/dishes. The specific implementation at EMBL allows for high resolution imaging via a high NA objective lens and is suited to thin 3D samples with a thickness of up to 30 \u00b5m. The volumetric imaging speed can be very high, allowing imaging of fast sub cellular processes on the timescale of seconds.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Sub-cellular spatial resolution, rapid all-optical volumetric imaging<\/li>\n\n\n\n<li>Dual-colour simultaneous imaging over &gt;100 \u00b5m diameter field of view&nbsp;<\/li>\n\n\n\n<li>Automated high-throughput, time-lapse imaging<\/li>\n\n\n\n<li>Temperature and gas control for living specimens (e.g. 37 C\/5% CO2)<\/li>\n\n\n\n<li>Built-in wide field epi-fluorescence microscope for \u2018scouting\u2019<\/li>\n\n\n\n<li>Light sheet dimensions can be tuned for the specimen to optimise sectioning vs. imaging depth<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Nikon 100x\/1.35 silicone oil objective&nbsp;<\/li>\n\n\n\n<li>3 lasers for fluorescence imaging (488, 561, 640 nm, Omicron LightHub Ultra)<\/li>\n\n\n\n<li>Back-thinned sCMOS camera (Photometrics Kinetix)<\/li>\n\n\n\n<li>Focus locking based on NIR laser (no interaction with the sample) (Toptica, iBeam Smart 808 nm)<\/li>\n\n\n\n<li>Motorised XYZ positioning stages (Physik Instrumente)<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-video\"><video style=\"max-width: 100%;\" height=\"1000\" style=\"aspect-ratio: 800 \/ 1000;\" width=\"800\" controls src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Test_Cropped.mp4\"><\/video><figcaption class=\"vf-figure__caption\">Live-cell imaging of the Golgi apparatus (blue) and histone H2B (orange) in the GaIT-EGFP h2b-mCherry cell line (Held et al.,&nbsp;Nature Methods, 2010). Cells were imaged over 48 hours of development at 30-minute intervals. Credit: Rory Power\/EMBL<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Oblique-Plane-Microscope-IC-2025-001-1024x683.jpg\" alt=\"\" class=\"wp-image-71151\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Oblique-Plane-Microscope-IC-2025-001-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Oblique-Plane-Microscope-IC-2025-001-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Oblique-Plane-Microscope-IC-2025-001-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Oblique-Plane-Microscope-IC-2025-001-1536x1024.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2026\/01\/Oblique-Plane-Microscope-IC-2025-001-2048x1365.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">OPM (oblique plane microscope). Credit: Kinga Lubowiecka\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-55ca62bd-2f7d-48c2-868e-5b89a7dd7512\"><h2>STELLARIS 8 STED Falcon<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>STELLARIS 8 STED with&nbsp;TauSTED&nbsp;from Leica Microsystems allows the study of multiple dynamic events simultaneously and investigation of molecular relationships and mechanisms within the cellular context.<\/p>\n\n\n\n<p>FALCON (FAst&nbsp;Lifetime&nbsp;CONtrast) includes phasors for quantitative FLIM analysis. It is a fully integrated solution for fluorescence lifetime imaging (FLIM) and enables video-rate image acquisition for rapid kinetic studies in live cells.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Resolving molecular relationships in specimens<\/li>\n\n\n\n<li>Multiple simultaneous events can be studied at the nanoscale<\/li>\n\n\n\n<li>2D and 3D STED imaging<\/li>\n\n\n\n<li>Significantly reduced light doses due to&nbsp;TauSTED<\/li>\n\n\n\n<li>Advanced STED-FLIM options with STED and FALCON (full FLIM quantitative tools, phasor FLIM, species separation based on phasor analysis)<\/li>\n\n\n\n<li>Advanced STED-FCS options<\/li>\n\n\n\n<li>Fast validation of results on a single platform, from confocal to super-resolution LIGHTNING and STED<\/li>\n\n\n\n<li>With FALCON it is possible to:\n<ul class=\"wp-block-list\">\n<li>follow fast molecular interactions via FLIM-FRET (F\u00f6rster&nbsp;resonance energy transfer)<\/li>\n\n\n\n<li>use biosensors to detect microenvironmental changes, such as pH or ion concentration<\/li>\n\n\n\n<li>apply lifetime contrast to separate multiple fluorophores<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>Analysis using phasor FLIM provides a 2D visualisation of lifetime components. With FLIM phasors you can follow microenvironmental changes, select components to multiplex signal (phasor separation), and determine FRET efficiency<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Five spectrally tunable Power&nbsp;HyD&nbsp;sensitive photon counting detectors (2&nbsp;HyD&nbsp;S, 2&nbsp;HyD&nbsp;X, 1&nbsp;HyD&nbsp;R)<\/li>\n\n\n\n<li>Confocal: tunable White Light Lasers (WLL) 440-790 nm, 405 nm STED depletion: 592 nm, 660 nm, 775 nm<\/li>\n\n\n\n<li>8kHz Resonant Scanner<\/li>\n\n\n\n<li>Total system dead-time: 1.5 ns<\/li>\n\n\n\n<li>TauSTED: Tunable resolution based on lifetime (depending on sample and fluorophore: &lt;30 nm (lateral) and &lt;100 nm (axial). Automatic lifetime-based background suppression algorithm. Light dose reduction (WLL excitation) for all STED lines (592, 660, 775 nm). Available for 2D and 3D STED in live and in fixed specimens, also for multicolor applications. Automated workflow integrated in the LAS X software<\/li>\n\n\n\n<li>The STED WHITE glycerol and water objective lenses with&nbsp;motCORR&nbsp;technology provide adaptive optical correction for aberrations introduced by sample inhomogeneities and refractive-index mismatch. The STED WHITE objective lenses provide a working distance of 300&nbsp;\u00b5m:\n<ul class=\"wp-block-list\">\n<li>HC PL APO 86x\/1.20 W&nbsp;motCORR&nbsp;STED WHITE<\/li>\n\n\n\n<li>HC PL APO 93x\/1.30 GLYC&nbsp;motCORR&nbsp;STED WHITE<\/li>\n\n\n\n<li>HC PL APO 100x\/1.40 OIL STED WHITE<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"541\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Stellaris-8-STED-Falcon-figure-new-541x1024.jpg\" alt=\"Credit: Timo Zimmermann\/EMBL.\" class=\"wp-image-4424\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Stellaris-8-STED-Falcon-figure-new-541x1024.jpg 541w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Stellaris-8-STED-Falcon-figure-new-158x300.jpg 158w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/Stellaris-8-STED-Falcon-figure-new.jpg 569w\" sizes=\"auto, (max-width: 541px) 100vw, 541px\" \/><figcaption class=\"vf-figure__caption\">U2OS cells: FLIM images under confocal and STED conditions of nuclear pore protein Nup96 and their corresponding phasor plots of lifetime information. TauSTED image of the same cell. Images taken with Leica Stellaris 8 STED Falcon. Credit: Timo Zimmermann\/EMBL.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_003-Edit-1024x683.jpg\" alt=\"Credit: Stuart Ingham\/EMBL.\" class=\"wp-image-4422\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_003-Edit-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_003-Edit-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_003-Edit-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_003-Edit.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Leica STELLARIS 8 STED Falcon. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-f7c3be18-6d8a-4021-a00d-1a8382e3163c\"><h2>MICA<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>Mica from Leica Microsystems is a highly automated microscope that unites widefield and confocal imaging. The user interface automates sample-finding, parameter setting and image focus, reducing imaging time. Through intelligent automation, all opto-digital components are fully motorized and automated, reducing the number of setup steps needed to generate images. The Mica has full environmental control capabilities.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Simultaneously visualize four colors in widefield or confocal, rapidly switching between modalities<\/li>\n\n\n\n<li>FluoSync\u2122 spectral unmixing<\/li>\n\n\n\n<li>Integrated incubation system<\/li>\n\n\n\n<li>On-board image analysis features (e.g pixel classifier and identification of parameters required for segmentation)<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Integrated modulation contrast (IMC) and brightfield transmitted light imaging in RGB or gray scale mode<\/li>\n\n\n\n<li>Incident fluorescence illumination: LED 365 nm, 470 nm, 555 nm, 625 nm<\/li>\n\n\n\n<li>4 simultaneous widefield detection channels with FluoSync spectral unmixing<\/li>\n\n\n\n<li>Confocal illumination: Laser diode 405 nm, 488 nm, 561 nm, 638 nm<\/li>\n\n\n\n<li>4 simultaneous confocal detection channels (HyD FS) with FluoSync spectral unmixing<\/li>\n\n\n\n<li>Environmental control: Temperature (room temperature +3 \u00b0C to 45 \u00b0C), CO2 (0 &#8211; 10 %), humidity<\/li>\n\n\n\n<li>Closed loop water dispenser for objective immersion. Water immersion for one objective is feedback controlled and does not require any user interaction<\/li>\n\n\n\n<li>THUNDER Methods: Instant Computational Clearing (ICC), Small Volume Computational Clearing (SVCC), Large Volume Computational Clearing (LVCC)<\/li>\n<\/ul>\n\n\n\n<p>LIGHTNING Methods Basic, LIGHTNING Expert<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"621\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/Golgi3zoom-1024x621.jpg\" alt=\"Credit: George Galea \/ EMBL\" class=\"wp-image-44875\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/Golgi3zoom-1024x621.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/Golgi3zoom-300x182.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/Golgi3zoom-768x466.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/Golgi3zoom-1536x932.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/Golgi3zoom-2048x1242.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Untreated Hela Kyoto cells stained to show the nucleus (Hoechst, blue), the cis-golgi matrix protein GM130 (AF488, green), and the trans-golgi network membrane protein TGN46 (AF647, red). Images were acquired on Mica with HC PL APO CS2 63x\/1.20 water objective using THUNDER grade and processing with +7 sample protection.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-88cadd0c-4e86-4adb-9d68-f766baf1354b\"><h2>THUNDER Imager Live Cell<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>THUNDER is an opto-digital technology from Leica Microsystems that uses the Computational Clearing method for haze removal to generate high-resolution and high-contrast images.&nbsp;<\/p>\n\n\n\n<p>A THUNDER Imager Live Cell&nbsp;provides a complete microscopy imaging system for minimally invasive and precise live-cell imaging. High-speed and\/or long term examination of specimens, e.g. cell cultures and organoids, can be performed at low phototoxicity and photobleaching.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>sCMOS&nbsp;technology<\/li>\n\n\n\n<li>Efficient removal of out-of-focus blur in real time with Computational Clearing<\/li>\n\n\n\n<li>Automation of the imaging and analysis workflow within the LAS X software<\/li>\n\n\n\n<li>Further processing and reconstruction using the&nbsp;Aivia&nbsp;software platform<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Based on the fully motorised, high-end, inverted research microscope DMi8<\/li>\n\n\n\n<li>High-speed positioning with the Quantum stage and Synapse real-time controller<\/li>\n\n\n\n<li>High-speed illumination with a multi-line LED light source<\/li>\n\n\n\n<li>Fast switching external filter wheel<\/li>\n\n\n\n<li>Adaptive Focus Control (AFC) with closed loop focus<\/li>\n\n\n\n<li>Climate chamber ensures optimal physiological conditions for living cells<\/li>\n<\/ul>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p>THUNDER Imager Live Cell is part of the Coral Life live cell CLEM workflow consisting of the THUNDER Imager Live Cell, the EM ICE high pressure freezer and the EM AFS2 for automatic freeze substitution.<\/p>\n\n\n\n<a href=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/clem-sample-preparation\/\" target=\"\">\n    <button class=\"vf-button vf-button--link\">\n        More details on CLEM sample preparation devices    <\/button>\n<\/a>\n<!--\/vf-button-->\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"716\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/11\/Vimentin_mCling_Thunder-detail-e1638454850639.jpg\" alt=\"Credit: Dietrich Walsh\/EMBL.\" class=\"wp-image-3872\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/11\/Vimentin_mCling_Thunder-detail-e1638454850639.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/11\/Vimentin_mCling_Thunder-detail-e1638454850639-300x210.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/11\/Vimentin_mCling_Thunder-detail-e1638454850639-768x537.jpg 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Hela cells: Vimentin (red), Membrane (green). Image before and after computational clearing. Image taken with Leica Thunder Imager Live Cell. Credit: Dietrich Walsh\/EMBL.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"641\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_092-Edit-1024x641.jpg\" alt=\"Credit: Stuart Ingham\/EMBL.\" class=\"wp-image-4498\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_092-Edit-1024x641.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_092-Edit-300x188.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_092-Edit-768x481.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_092-Edit.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Leica THUNDER Imager Live Cell. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-297bd44f-71d5-4404-aada-ab6edf34f3c4\"><h2>DMi8 S with TIRF module<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>DMi8 S from Leica Microsystems is a live cell imaging system to execute advanced experiments with imaging modalities like TIRF.&nbsp; For dynamic processes at the cell surface TIRF (Total Internal Reflection Fluorescence) microscopy is the method of choice to visualise single molecules with super-resolution by maximising the fluorescent signal- to-noise-ratio. <\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p>DMi8 S from Leica Microsystems is a live cell imaging system to execute advanced experiments with imaging modalities like TIRF.&nbsp; For dynamic processes at the cell surface TIRF (Total Internal Reflection Fluorescence) microscopy is the method of choice to visualise single molecules with super-resolution by maximising the fluorescent signal- to-noise-ratio. <\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Infinity TIRF module with azimuth shifting capability to optimise the illumination field.<\/li>\n\n\n\n<li>LAS X Navigator software to create quick overviews of the sample and identify the important details instantly. High resolution image acquisition can be set up using templates for slides, dishes, and multi-well plates.<\/li>\n<\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fully motorised DMi8 inverted research microscope with integrated real-time controller to operate the system with microsecond precision<\/li>\n\n\n\n<li>Adaptive Focus Control (AFC)<\/li>\n\n\n\n<li>Equipped with a high-end sCMOS camera<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"960\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/DMI8-Widefield-TIRF-comparison-960x1024.jpg\" alt=\"Credit: Dietrich Walsh\/EMBL\" class=\"wp-image-15072\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/DMI8-Widefield-TIRF-comparison-960x1024.jpg 960w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/DMI8-Widefield-TIRF-comparison-281x300.jpg 281w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/DMI8-Widefield-TIRF-comparison-768x819.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/DMI8-Widefield-TIRF-comparison.jpg 992w\" sizes=\"auto, (max-width: 960px) 100vw, 960px\" \/><figcaption class=\"vf-figure__caption\">Hela cells: Microtubules (magenta); Mitochondria (cyan). Comparison of widefield and TIRF imaging in living cells. Image taken with Leica DMi8 S Infinity TIRF. Credit: Dietrich Walsh\/EMBL.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/IC_Equipment_110-Edit-1024x683.jpg\" alt=\"Credit: Stuart Bailey\/EMBL.\" class=\"wp-image-15088\" style=\"object-fit:cover\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/IC_Equipment_110-Edit-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/IC_Equipment_110-Edit-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/IC_Equipment_110-Edit-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/IC_Equipment_110-Edit-1536x1025.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2022\/11\/IC_Equipment_110-Edit-2048x1366.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">DMi8 S with TIRF module at EMBL IC.Credit: Stuart Bailey\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":10,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-44827","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/44827","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=44827"}],"version-history":[{"count":19,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/44827\/revisions"}],"predecessor-version":[{"id":72393,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/44827\/revisions\/72393"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=44827"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=44827"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}