{"id":34087,"date":"2024-01-29T11:25:58","date_gmt":"2024-01-29T11:25:58","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=34087"},"modified":"2024-07-03T10:58:13","modified_gmt":"2024-07-03T10:58:13","slug":"34087-2","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/34087-2\/","title":{"rendered":""},"content":{"rendered":"\n<figure class=\"vf-figure wp-block-image size-full\"><img decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/Logo-course3.tif\" alt=\"\" class=\"wp-image-34095\"\/><\/figure>\n\n\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-e4f74508-a8df-467f-886f-74e3d8af7d5d\" data-vf-js-location-nearest-activation-target=\"\">General Info<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-d72487b2-fe1b-4000-aa65-02ae3aa08cb1\" data-vf-js-location-nearest-activation-target=\"\">Lecture\/Practical Content<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-3826f559-9052-4a9c-9d65-abc6be32dac7\" data-vf-js-location-nearest-activation-target=\"\">Programme<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-1abf9384-a304-4e1d-8842-ddddc95cefdb\" data-vf-js-location-nearest-activation-target=\"\">Practical Information <\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-e4f74508-a8df-467f-886f-74e3d8af7d5d\"><h2 class=\"vf-u-sr-only\">General Info<\/h2>\n<p>EMBL Imaging Centre in collaboration with Heidelberg University is offering a 4-day summer school diving into basics of focused ion beam \u2013 scanning electron microscopy methods under cryogenic (cryo-FIB-SEM), which allows to study biological samples in their native environment at high-resolution.<\/p>\n\n\n\n<p>Content of the course: <\/p>\n\n\n\n<p>\u2022&nbsp; Theoretical background and practicals delivered by experts in the field<\/p>\n\n\n\n<p>\u2022&nbsp; Sample preparation, lamella preparation and cryo-electron tomography<\/p>\n\n\n\n<p>\u2022&nbsp; Open equipment day to explore further<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>External invited speaker:  <\/p>\n\n\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/Florent-Waltz-300x300.jpeg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/Florent-Waltz-300x300.jpeg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/Florent-Waltz-150x150.jpeg 150w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/Florent-Waltz.jpeg 512w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\n    <h3 class=\"vf-profile__title\" >\n                    <a href=\"https:\/\/www.biozentrum.unibas.ch\/research\/research-groups\/research-groups-a-z\/group\/unit\/research-group-ben-engel\" target=\"_self\" class=\"vf-profile__link\">Florent Waltz<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" >\n                University of Basel            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n\n\n<p>Organisers: <\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"vf-content-hub-html\" data-cache=\"131fa57a\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-contains%5Bfield_person_full_name%5D=Steyer&amp;filter-fields-empty=field_person_visible_internally&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bposition%2Cemail%2Corcid%5D=1&amp;limit=1&amp;pattern=vf-profile-inline -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"108398\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60017365.jpg?itok=x4ylfaXh\" alt=\"image of Anna Steyer\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/anna-steyer\" class=\"vf-profile__link\">Anna Steyer<\/a>\n                  <\/h3>\n      \n      \n      \n              <p class=\"vf-profile__text\">\n          Mattei Team\n        <\/p>\n      \n              \n              \n      \n      \n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/108398\/108398\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"vf-content-hub-html\" data-cache=\"50a623e1\">\n      <!-- Generated by: http:\/\/content.embl.org\/api\/v1\/pattern.html?filter-content-type=person&amp;filter-field-contains%5Bfield_person_full_name%5D=Mattei&amp;filter-fields-empty=field_person_visible_internally&amp;filter-ref-entity%5Bfield_person_positions%5D%5Btitle%5D=&amp;filter-ref-entity%5Bfield_person_positions%5D%5Bfield_position_primary%5D=1&amp;hide%5Bposition%2Cemail%2Corcid%5D=1&amp;limit=1&amp;pattern=vf-profile-inline -->\n                \n                            <article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline\" data-embl-js-conditional-edit=\"88796\">\n              <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/content.embl.org\/\/sites\/default\/files\/styles\/medium\/public\/persons\/CP-60016086.jpg?itok=JaLxGDkU\" alt=\"image of Simone Mattei\" \/>\n      \n              <h3 class=\"vf-profile__title\">\n                      <a href=\"https:\/\/www.embl.org\/people\/person\/simone-mattei\" class=\"vf-profile__link\">Simone Mattei<\/a>\n                  <\/h3>\n      \n      \n      \n              <p class=\"vf-profile__text\">\n          Mattei Team\n        <\/p>\n      \n              \n              \n      \n      \n            <a class=\"vf-text vf-text--body-r vf-link embl-conditional-edit\" rel=\"noopener noreferrer nofollow\" href=\"\/node\/88796\/88796\" target=\"_blank\">\n        Edit\n      <\/a>\n    <\/article>\n  <\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--inline | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"300\" height=\"284\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/128_crop-scaled-e1705931630921-300x284.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/128_crop-scaled-e1705931630921-300x284.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/128_crop-scaled-e1705931630921-1024x970.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/128_crop-scaled-e1705931630921-768x728.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/128_crop-scaled-e1705931630921-1536x1455.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/128_crop-scaled-e1705931630921-2048x1940.jpg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\n    <h3 class=\"vf-profile__title\" >\n                    <a href=\"https:\/\/www.bioquant.uni-heidelberg.de\/members\/petr-chlanda.html\" target=\"_self\" class=\"vf-profile__link\">Petr Chlanda <\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" >\n                BioQuant &#8211; Heidelberg University            <\/p>\n    \n    \n      \n\n    \n<\/article>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:32px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Contact: Anna Steyer anna.steyer@embl.de<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>To participate to the summer school please register by clicking on the following button. Participation is limited to 9 participants.<strong> Deadline for registration is April 1st 2024<\/strong>. <\/p>\n\n\n\n<p>Selected participants will be announced on April 8th 2024. <\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<a href=\"https:\/\/easy-feedback.com\/SummerSchoolCryo-FIB2024\/1772049\/W8aI07\" target=\"\">\n    <button class=\"vf-button vf-button--primary\">\n        Register here!     <\/button>\n<\/a>\n<!--\/vf-button-->\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-top is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Sponsor: <\/p>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"258\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/logo-Alliance-HD-e1706527212264-1024x258.png\" alt=\"\" class=\"wp-image-34617\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/logo-Alliance-HD-e1706527212264-1024x258.png 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/logo-Alliance-HD-e1706527212264-300x76.png 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/logo-Alliance-HD-e1706527212264-768x193.png 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/01\/logo-Alliance-HD-e1706527212264.png 1445w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-d72487b2-fe1b-4000-aa65-02ae3aa08cb1\"><h2 class=\"vf-u-sr-only\">Lecture\/Practical Content<\/h2>\n<p><strong>Lectures:<\/strong><br><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Florent Waltz (external speaker, university of Basel):&nbsp;<em>Exploring the architecture of the cell at high-resolution.&nbsp;<\/em>The lecture will dive into different molecular machines at high-resolution using cryo-FIB-SEM as well as cryo-tomography.<\/li>\n\n\n\n<li>Petr Chlanda (University of Heidelberg):&nbsp;<em>Cryo-CLEM: Structural analysis of virus-infected cells.<\/em> This lecture will demonstrate how the combination of cryo-LM and cryo-EM can be used to perform structural analysis on pathogens.<\/li>\n\n\n\n<li>Georg Wolff (EMBL):&nbsp;<em>Plunging vs. high-pressure: How to prepare your FIB-SEM sample?&nbsp;<\/em>General basics of sample preparation methods covering the vitrification part of small specimen using a plunge freezer or larger specimen using high-pressure freezing.<\/li>\n\n\n\n<li>Zhengyi Yang (EMBL):&nbsp;<em>Cryo-Tomography: Tools and concepts in cryo electron tomography.&nbsp;<\/em>The lecture will dive into the basic principles of cryo-tomography, what can be achieved with it and a general overview of pipelines, including software that could be used.<\/li>\n\n\n\n<li>Anna Steyer (EMBL):&nbsp;<em>Breaking the ice: Basic principals of the FIB-SEM in cryo.&nbsp;<\/em>General basics of the Focused ion beam \u2013 scanning electron microscope hardware and software related will be given as well as practical aspects of lamella preparation.<\/li>\n<\/ol>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>Practicals:<\/strong><\/p>\n\n\n\n<p><br>Practical 1: Sample preparation (Plunge freezing and high-pressure freezing)<\/p>\n\n\n\n<p>High-resolution electron microscopy requires well vitrified specimen. Depending on the thickness of the sample this can be either accomplished by plunge freezing for thinner specimen, up to a few \u00b5m or high-pressure freezing up to ~200 \u00b5m.<\/p>\n\n\n\n<p>Transmission electron microscopy (TEM) grids are plasma cleaned to make them less hydrophobic, allowing an even spread of sample. Two different plunge freezers allowing one-side and two-sided blotting used for cells or purified proteins are used to vitrify cells\/protein suspensions. A high-pressure freezer is used to freeze thicker specimen e.g.&nbsp;<em>C elegans.<\/em><\/p>\n\n\n\n<p>The participants will have the opportunity to use:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Vitrobot Mark 4 (Thermo Fischer Scientific) for freezing protein suspensions<\/li>\n\n\n\n<li>GP2 (Leica Microsystems) for freezing adherent cells<\/li>\n\n\n\n<li>EM ICE (Leica Microsystems) high-pressure freezer to vitrify&nbsp;<em>C. elegans<\/em>&nbsp;in metal carrier<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<p>Practical 2: Lamella preparation: Serial-FIB\/AutoTEM\/iFLM<\/p>\n\n\n\n<p>After sample vitrification the TEM grid is introduced into the FIB-SEM and the region of interest is identified. Different milling steps with decreasing beam currents are applied to gradually remove cell material (starting from 1 nA down to 30 pA). Understanding the geometry and cutting approaches while practicing on a real sample will give the best understanding of the challenges, throughput and the possibilities imposed by this method.<\/p>\n\n\n\n<p>The participants will have the opportunity to use:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>ThermoScientific Aquilos to prepare lamellae using SerialFIB<\/li>\n\n\n\n<li>ThermoScientific Aquilos 2 with an integrated fluorescence light microscope (iFLM) to prepare lamellae<\/li>\n\n\n\n<li>Zeiss Crossbeam 550 to prepare lamellae using SerialFIB<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<p>Practical 3: Cryo-Tomography setup<\/p>\n\n\n\n<p>The TEM grid is introduced into the transmission electron microscope and an overview image of the full grid is taken. After identifying the regions with the lamellae, medium magnification montages are taken to acquire images of the lamella being able to identify and target regions of interest for high-resolution tilt series.<\/p>\n\n\n\n<p>The participants will have the opportunity to use:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>ThermoScientific Titan Krios<\/li>\n<\/ul>\n\n\n\n<p>Practical 4: Open equipment practical<\/p>\n\n\n\n<p>This practical will allow everybody to spend some more time practice operating an instrument of their choice.<\/p>\n\n\n\n<p>The participants will have the opportunity to use all the equipment used during duration of the course.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-3826f559-9052-4a9c-9d65-abc6be32dac7\"><h2 class=\"vf-u-sr-only\">Programme<\/h2>\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 1 &#8211; Monday 17th June 2024<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-stripes\"><table><thead><tr><th>Time<br>(Europe\/Berlin)<\/th><th>Speaker<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>9:00-9:15<\/td><td><strong>Welcome and Introduction <\/strong><br>Simone Mattei &#8211; EMBL <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>9:15-10:00<\/td><td>Round table introductions<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>10:00-10:15<\/td><td>Q&amp;A <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>10:15-10:45<\/td><td>Coffee Break <\/td><td><\/td><\/tr><tr><td>10:45-11:30 <\/td><td><strong>Breaking the ice: Basic principals of the FIB-SEM in cryo<\/strong><br>Anna Steyer &#8211; EMBL<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>11:30-11:45 <\/td><td>Q&amp;A<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>11:45-13:00<\/td><td>Lunch break <\/td><td>EMBL Canteen<\/td><\/tr><tr><td>13:15-18:15<\/td><td><strong>Practical on Sample preparation plunge freezing and high-pressure freezing<\/strong> (sample preparation rotations of 90&#8242;)<\/td><td>EMBL Imaging Centre <br>Labs<\/td><\/tr><tr><td>19:00 <\/td><td>Dinner<\/td><td>Restaurant Kulturbrauerei<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 2 &#8211; Tuesday 18th June 2024<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-stripes\"><table><thead><tr><th>Time<br>(Europe\/Berlin)<\/th><th>Speaker<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>9:00-9:15<\/td><td>Feedback discussion and exchanges on practicals <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>9:15-10:00<\/td><td><strong>Plunging vs. high-pressure: How to prepare your FIB-SEM sample? <\/strong><br>Georg Wolff &#8211; EMBL <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>10:00-10:15<\/td><td>Q&amp;A <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>10:15-10:45<\/td><td>Coffee Break <\/td><td><\/td><\/tr><tr><td>10:45-11:30 <\/td><td><strong>Cryo-CLEM: Structural analysis of virus -infected cells. <\/strong><br>Petr Chlanda &#8211; Heidelberg University <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>11:30-11:45 <\/td><td>Q&amp;A <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>11:45-13:00<\/td><td>Lunch break <\/td><td>EMBL Canteen<\/td><\/tr><tr><td>13:15-18:15<\/td><td><strong>Practical on lamella preparation with a cryo-FIB instrument<\/strong> (lamella preparation rotations of 90&#8242;)<\/td><td>EMBL Imaging Centre <br>Labs<\/td><\/tr><tr><td>19:00<\/td><td>Free evening<\/td><td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 3 &#8211; Wednesday 19th June 2024<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-stripes\"><table><thead><tr><th>Time<br>(Europe\/Berlin)<\/th><th>Speaker<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>9:00-9:15<\/td><td>Feedback discussion and exchanges on practicals<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>9:15-10:00<\/td><td><strong>Cryo-Tomography: Tools and concepts in cryo electron tomography<\/strong>. <br>Zhengyi Yang &#8211; EMBL <\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>10:00-10:15<\/td><td>Q &amp; A<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>10:15-10:45<\/td><td>Coffee break<\/td><td><\/td><\/tr><tr><td>10:45-11:30<\/td><td><strong>Exploring the architecture of the cell at high-resolution.<\/strong><br>Florent Waltz &#8211; University of Basel<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>11:30-11:45<\/td><td>Q &amp; A<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>11:45-13:00<\/td><td>Lunch break<\/td><td>EMBL Canteen<\/td><\/tr><tr><td>13:15-18:15<\/td><td><strong>Practical cryo electron tomography data collection<\/strong> (cryo-tomography setup)<\/td><td>EMBL Imaging Centre <br>Labs<\/td><\/tr><tr><td>19:00<\/td><td>Free evening<\/td><td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 4 &#8211; Thursday 20th June 2024 <\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-stripes\"><table><thead><tr><th>Time<br>(Europe\/Berlin)<\/th><th>Speaker<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>9:00-9:15<\/td><td>Feedback discussion and exchanges on practicals<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><tr><td>9:15-10:15<\/td><td><strong>Pratical Open Equipment &#8211; free Pratical slot<\/strong><\/td><td>EMBL Imaging Centre <br>Labs<\/td><\/tr><tr><td>10:15-10:45<\/td><td>Coffee break<\/td><td><\/td><\/tr><tr><td>10:45-11:45<\/td><td><strong>Pratical Open Equipment &#8211; free Pratical slot<\/strong><\/td><td>EMBL Imaging Centre <br>Labs<\/td><\/tr><tr><td>11:45-13:00<\/td><td>Lunch break<\/td><td>EMBL Canteen<\/td><\/tr><tr><td>13:15-14:45<\/td><td>Feedback and Closing remarks<\/td><td>EMBL Imaging Centre <br>room 15-104<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-1abf9384-a304-4e1d-8842-ddddc95cefdb\"><h2>Practical Information <\/h2>\n<p>The course is <strong>limited to 9 participants<\/strong>. For selection purposes, please note that your application will not be considered without CV and a letter of motivation, stating your background and how this course would be beneficial for your future work.<\/p>\n\n\n\n<p>Selected participants will be announced on <strong>April 8th 2024<\/strong>. <\/p>\n\n\n\n<p>There is <strong>no registration fee<\/strong>.<\/p>\n\n\n\n<p>Contact: Anna Steyer anna.steyer@embl.de<\/p>\n<\/section>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":16,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-34087","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/34087","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=34087"}],"version-history":[{"count":98,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/34087\/revisions"}],"predecessor-version":[{"id":42607,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/34087\/revisions\/42607"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=34087"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=34087"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}