{"id":3260,"date":"2021-09-17T13:36:00","date_gmt":"2021-09-17T13:36:00","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=3260"},"modified":"2026-01-13T15:07:50","modified_gmt":"2026-01-13T15:07:50","slug":"cellular-tomography","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/cellular-tomography\/","title":{"rendered":"Cellular cryo-tomography"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-1\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-644f1b78-6d9e-4ed0-a592-5b29c3dab209\" data-vf-js-location-nearest-activation-target=\"\">Aquilos 2<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-46e411be-b58c-4290-9ef3-82a78925ed84\" data-vf-js-location-nearest-activation-target=\"\">Titan Krios G4<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-644f1b78-6d9e-4ed0-a592-5b29c3dab209\"><h2>Aquilos 2<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Aquilos 2 (focused ion beam \u2013 scanning electron microscope) from Thermo Fisher Scientific enables the preparation of cellular lamellae from vitreous samples suitable for high-resolution cryo-electron tomography analyses. Plunge frozen as well as high-pressure frozen (HPF) samples can be processed at cryo temperature by standard FIB milling and cryo-lift out approaches, respectively. The lamellae production and lift out process can be guided with fluorescence signal, either performed on a cryo-light microscope prior to the session, or with integrated fluorescence microscope in Aquilos 2.Automated FIB milling routines based on commercial and in-house developed software packages enable unsupervised overnight operations for high-throughput milling.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Important specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Serial-FIB and AutoTEM software for automated lamella preparation<\/li>\n\n\n\n<li>EasyLift NanoManipulator<\/li>\n\n\n\n<li>Integrated Fluorescence Light Microscope (iFLM)<\/li>\n<\/ul>\n\n\n\n<p><strong>Offered workflows<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Automated over-night lamella preparation on plunge frozen samples.<\/li>\n\n\n\n<li>Waffle milling on HPF waffle grids.<\/li>\n\n\n\n<li>Serial lift out on HPF waffle or planchette carriers, followed with automated lamellae prepraration.<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"2032\" height=\"1213\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007.jpg\" alt=\"Lamella through plunge frozen yeast cells imaged with the iFLM inside the Aquilos 2. Credit Zhengyi Yang \/ EMBL.\" class=\"wp-image-45235\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007.jpg 2032w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-300x179.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-1024x611.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-768x458.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-1536x917.jpg 1536w\" sizes=\"auto, (max-width: 2032px) 100vw, 2032px\" \/><figcaption class=\"vf-figure__caption\">Lamella through plunge frozen yeast cells imaged with the iFLM inside the Aquilos 2<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-1024x683.jpg\" alt=\"\" class=\"wp-image-36657\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-1536x1025.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-2048x1366.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Aquilos 2. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-46e411be-b58c-4290-9ef3-82a78925ed84\"><h2>Titan Krios G4<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Titan Krios G4 from Thermo Fisher Scientific is a 300 kV transmission electron microscope (TEM) with a three-condenser lens system. It features the latest technology for high-end single-particle and tomography data acquisition including a cold field emission gun (C-FEG), SelectrisX energy filter, Falcon 4 electron counting camera and volta phase plate. It\u2019s configured for high-throughput automated single particle and tomography data acquisition.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Unique features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>C-FEG electron source with narrow energy spread<\/li>\n\n\n\n<li>State-of-the-art energy filter and electron counting camera<\/li>\n\n\n\n<li>phase plate<\/li>\n\n\n\n<li>Autoloader system for easy sample transfer between TFS TEMs<\/li>\n<\/ul>\n\n\n\n<p><strong>Important specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>300 kV C-FEG electron source<\/li>\n\n\n\n<li>SelectrisX energy filter<\/li>\n\n\n\n<li>Falcon 4EC direct electron detector<\/li>\n\n\n\n<li>Volta phase plate<\/li>\n\n\n\n<li>SerialEM software for tailored, automated data acquisition<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/krios_image01.png\" alt=\"\" class=\"wp-image-3110\"\/><figcaption class=\"vf-figure__caption\"><em>Cryo-EM density map after 3D reconstruction with an atomic coordinate model fitted.<\/em><\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1019\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-1024x1019.jpg\" alt=\"Credit: Stuart Ingham\/EMBL.\" class=\"wp-image-4616\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-1024x1019.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-300x300.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-150x150.jpg 150w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-768x764.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Titan Krios. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-1\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-3260","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=3260"}],"version-history":[{"count":20,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3260\/revisions"}],"predecessor-version":[{"id":71145,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3260\/revisions\/71145"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=3260"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=3260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}