{"id":3102,"date":"2021-09-17T10:09:49","date_gmt":"2021-09-17T10:09:49","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=3102"},"modified":"2022-06-20T08:18:06","modified_gmt":"2022-06-20T08:18:06","slug":"high-end-data-collection","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/high-end-data-collection\/","title":{"rendered":"High-End Data Collection"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-1\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-f7a77b3f-ce92-44e0-9cf6-190701f505a3\" data-vf-js-location-nearest-activation-target=\"\">Titan Krios G4<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-fb851827-c6ee-44a3-97a5-8a4b3257f48b\" data-vf-js-location-nearest-activation-target=\"\">Glacios<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-f7a77b3f-ce92-44e0-9cf6-190701f505a3\"><h2>Titan Krios G4<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Titan Krios G4 from Thermo Fisher Scientific is a 300 kV transmission electron microscope (TEM) with a three-condenser lens system. It features the latest technology for high-end single-particle and tomography data acquisition including a cold field emission gun (C-FEG), SelectrisX energy filter, Falcon 4 electron counting camera and volta phase plate. It\u2019s configured for high-throughput automated single particle and tomography data acquisition.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>C-FEG electron source with narrow energy spread<\/li><li>State-of-the-art energy filter and electron counting camera<\/li><li>phase plate<\/li><li>Autoloader system for easy sample transfer between TFS TEMs<\/li><\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>300 kV C-FEG electron source<\/li><li>SelectrisX energy filter<\/li><li>Falcon 4EC direct electron detector<\/li><li>Volta phase plate<\/li><li>SerialEM software for tailored, automated data acquisition<\/li><\/ul>\n\n\n\n<div style=\"height:19px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/krios_image01.png\" alt=\"\" class=\"wp-image-3110\"\/><figcaption class=\"vf-figure__caption\"><em>Cryo-EM density map after 3D reconstruction with an atomic coordinate model fitted.<\/em><\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1019\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-1024x1019.jpg\" alt=\"Credit: Stuart Ingham\/EMBL.\" class=\"wp-image-4616\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-1024x1019.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-300x300.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-150x150.jpg 150w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2-768x764.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_202-Edit-Edit-Edit-Edit-2.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Titan Krios. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-fb851827-c6ee-44a3-97a5-8a4b3257f48b\"><h2>Glacios<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Glacios from Thermo Fisher Scientific is a 200 kV transmission electron microscope with a two-condenser lens system. It\u2019s equipped with an autoloader system which allows for quick screening of up to 12 cryo-EM samples. The system also features the latest state-of-the-art SelectrisX energy filter and Falcon 4 electron counting camera which enables high-quality data collection for suitable single particle samples.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Unique features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Autoloader system for easy sample transfer between TFS TEMs<\/li><li>State-of-the-art energy filter and electron counting camera<\/li><\/ul>\n\n\n\n<p><strong>Important specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>200 kV FEG electron source<\/li><li>SelectrisX energy filter<\/li><li>Falcon 4EC direct electron detector<\/li><li>SerialEM software for tailored, automated data acquisition<\/li><\/ul>\n\n\n\n<div style=\"height:19px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"793\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651.png\" alt=\"\" class=\"wp-image-3086\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651.png 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651-300x232.png 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651-768x595.png 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Cryo-EM micrograph of an exemplary single-particle sample.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-1024x683.jpg\" alt=\"Credit: Stuart Ingham\/EMBL\" class=\"wp-image-4624\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Glacios. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-3102","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3102","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=3102"}],"version-history":[{"count":16,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3102\/revisions"}],"predecessor-version":[{"id":7822,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3102\/revisions\/7822"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=3102"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=3102"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}