{"id":3090,"date":"2021-09-17T09:57:36","date_gmt":"2021-09-17T09:57:36","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=3090"},"modified":"2026-01-13T15:05:14","modified_gmt":"2026-01-13T15:05:14","slug":"cryo-fib-milling-for-lamella-preparation","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/cryo-fib-milling-for-lamella-preparation\/","title":{"rendered":"Cryo-FIB Milling for Lamella Preparation"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-1\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-2076abb1-22a5-4086-92b5-3a6861bcc5dd\" data-vf-js-location-nearest-activation-target=\"\">Aquilos 2<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-2076abb1-22a5-4086-92b5-3a6861bcc5dd\"><h2>Aquilos 2<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Aquilos 2 (focused ion beam \u2013 scanning electron microscope) from Thermo Fisher Scientific enables the preparation of cellular lamellae from vitreous samples suitable for high-resolution cryo-electron tomography analyses. Plunge frozen as well as high-pressure frozen (HPF) samples can be processed at cryo temperature by standard FIB milling and cryo-lift out approaches, respectively. The lamellae production and lift out process can be guided with fluorescence signal, either performed on a cryo-light microscope prior to the session, or with integrated fluorescence microscope in Aquilos 2. Automated FIB milling routines based on commercial and in-house developed software packages enable unsupervised overnight operations for high-throughput milling.<\/p>\n\n\n\n<p><strong>Important specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Serial-FIB and AutoTEM software for automated lamella preparation<\/li>\n\n\n\n<li>EasyLift NanoManipulator<\/li>\n\n\n\n<li>Integrated Fluorescence Light Microscope (iFLM)<\/li>\n<\/ul>\n\n\n\n<p><strong>Offered workflows<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Automated over-night lamella preparation on plunge frozen samples.<\/li>\n\n\n\n<li>Waffle milling on HPF waffle grids.<\/li>\n\n\n\n<li>Serial lift out on HPF waffle or planchette carriers, followed with automated lamellae prepraration.<\/li>\n<\/ul>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"2032\" height=\"1213\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007.jpg\" alt=\"Lamella through plunge frozen yeast cells imaged with the iFLM inside the Aquilos 2. Credit Zhengyi Yang \/ EMBL.\" class=\"wp-image-45235\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007.jpg 2032w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-300x179.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-1024x611.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-768x458.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/08\/screenshot_007-1536x917.jpg 1536w\" sizes=\"auto, (max-width: 2032px) 100vw, 2032px\" \/><figcaption class=\"vf-figure__caption\">Lamella through plunge frozen yeast cells imaged with the iFLM inside the Aquilos 2.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-1024x683.jpg\" alt=\"\" class=\"wp-image-36657\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-1536x1025.jpg 1536w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2024\/03\/Imaging-Centre-_Aquilos-2-Edit-2048x1366.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Aquilos 2. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-3090","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3090","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=3090"}],"version-history":[{"count":15,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3090\/revisions"}],"predecessor-version":[{"id":71141,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3090\/revisions\/71141"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=3090"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=3090"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}