{"id":3072,"date":"2021-09-17T13:06:48","date_gmt":"2021-09-17T13:06:48","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=3072"},"modified":"2022-06-20T08:17:56","modified_gmt":"2022-06-20T08:17:56","slug":"sample-vitrification-screening","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/sample-vitrification-screening\/","title":{"rendered":"Sample Vitrification &#038; Screening"},"content":{"rendered":"\n<div class=\"vf-grid | vf-grid__col-1\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-cf31a4a6-f92f-4ac3-95df-41727f8df1fe\" data-vf-js-location-nearest-activation-target=\"\">Vitrobot Mark IV<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-97af3868-1fc7-4270-b4b8-e45d2014bdd4\" data-vf-js-location-nearest-activation-target=\"\">EM GP2<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-f7a77b3f-ce92-44e0-9cf6-190701f505a3\" data-vf-js-location-nearest-activation-target=\"\">Glacios<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-cf31a4a6-f92f-4ac3-95df-41727f8df1fe\"><h2>Vitrobot Mark IV<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Vitrobot Mark IV System from Thermo Fisher Scientific is an automated plunge freezer able to vitrify thin samples applied to an electron microscopy grid after removing excess fluid by blotting.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><\/p>\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Automated vitrification of aqueous suspensions and cells deposited on EM grids<\/li><li>Temperature and humidity-controlled environmental chamber<\/li><\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Working temperature adjustable between +4\u00b0C and +60\u00b0C<\/li><li>Relative humidity adjustable to 100%<\/li><\/ul>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"647\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_224-Edit-Edit-647x1024.jpg\" alt=\"Credit: Stuart Ingham\/EMBL.\" class=\"wp-image-4640\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_224-Edit-Edit-647x1024.jpg 647w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_224-Edit-Edit-190x300.jpg 190w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_224-Edit-Edit.jpg 758w\" sizes=\"auto, (max-width: 647px) 100vw, 647px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Vitrobot Mark IV. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-97af3868-1fc7-4270-b4b8-e45d2014bdd4\"><h2>EM GP2<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The EM GP2 from Leica Microsystems is an automated plunge freezer able to vitrify thin samples applied to an electron microscopy grid after removing excess fluid by blotting.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Automated vitrification of aqueous suspensions and cells deposited on EM grids<\/li><li>Temperature and humidity-controlled environmental chamber<\/li><li>Sensor-driven blotting<\/li><\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Working temperature adjustable between +4\u00b0C and +60\u00b0C<\/li><li>Relative humidity adjustable to 99%<\/li><\/ul>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large is-style-default\"><a href=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_086-Edit.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"781\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_086-Edit-781x1024.jpg\" alt=\"Credit: Stuart Ingham\/EMBL.\" class=\"wp-image-4568\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_086-Edit-781x1024.jpg 781w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_086-Edit-229x300.jpg 229w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_086-Edit-768x1007.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_086-Edit.jpg 915w\" sizes=\"auto, (max-width: 781px) 100vw, 781px\" \/><\/a><figcaption class=\"vf-figure__caption\">Leica EM GP2. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n\n\n<p><\/p>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-f7a77b3f-ce92-44e0-9cf6-190701f505a3\"><h2>Glacios<\/h2>\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"vf-grid__col--span-2\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p>The Glacios from Thermo Fisher Scientific is a 200 kV transmission electron microscope with a two-condenser lens system. It\u2019s equipped with an autoloader system which allows for quick screening of up to 12 cryo-EM samples. The system also features the latest state-of-the-art SelectrisX energy filter and Falcon 4 electron counting camera which enables high-quality data collection for suitable single particle samples.<\/p>\n\n\n\n<hr class=\"vf-divider\">\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Autoloader system for easy sample transfer between TFS TEMs<\/li><li>State-of-the-art energy filter and electron counting camera<\/li><\/ul>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>200 kV FEG electron source<\/li><li>SelectrisX energy filter<\/li><li>Falcon 4EC direct electron detector<\/li><li>SerialEM software for tailored, automated data acquisition<\/li><\/ul>\n\n\n\n<div style=\"height:19px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"793\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651.png\" alt=\"\" class=\"wp-image-3086\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651.png 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651-300x232.png 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/09\/glacios_image01-e1638462254651-768x595.png 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Cryo-EM micrograph of an exemplary single-particle sample.<\/figcaption><\/figure>\n\n\n\n<figure class=\"vf-figure wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-1024x683.jpg\" alt=\"Credit: Stuart Ingham\/EMBL\" class=\"wp-image-4624\" srcset=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-1024x683.jpg 1024w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-300x200.jpg 300w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2-768x512.jpg 768w, https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2021\/12\/IC_Equipment_188-Edit-2.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"vf-figure__caption\">Thermo Fisher Scientific Glacios. Credit: Stuart Ingham\/EMBL.<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-title-left-aligned.php","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-3072","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3072","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=3072"}],"version-history":[{"count":16,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3072\/revisions"}],"predecessor-version":[{"id":9034,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/3072\/revisions\/9034"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=3072"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=3072"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}