{"id":230,"date":"2020-11-09T12:55:44","date_gmt":"2020-11-09T12:55:44","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/?page_id=230"},"modified":"2020-11-26T07:39:24","modified_gmt":"2020-11-26T07:39:24","slug":"matthias-schmidt","status":"publish","type":"page","link":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/user-stories\/matthias-schmidt\/","title":{"rendered":"Matthias Schmidt&#8217;s story"},"content":{"rendered":"\n<div class=\"embl-grid embl-grid--has-centered-content\"><div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"wp-block-image\"><figure class=\"vf-figure  | vf-figure--align vf-figure--align-inline-end  size-large\"><img decoding=\"async\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-content\/uploads\/2020\/11\/Matthias-Schmidt.jpg\" alt=\"\" class=\"wp-image-238\"\/><figcaption class=\"vf-figure__caption\"><em>Matthias Schmidt<\/em><br><em>PHOTO: Ulm University<\/em><\/figcaption><\/figure><\/div>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p class=\"wp-block-paragraph\"><strong>Ulm University<\/strong><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Why did you decide to come to EMBL\u2019s cryo-EM service platform?<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">In our lab we work on the structural analysis of different amyloid fibrils, including fibrils from Alzheimer&#8217;s disease. But at the moment our focus is mainly on&nbsp;<em>ex vivo<\/em> fibrils from systemic amyloidosis (AA-amyloidosis and AL-amyloidosis). I&#8217;m working with different amyloid fibrils and I try to reconstruct them at high resolution to create an atomic model.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">At our university we have two EMs made by JEOL, which we use for our work, a JEM-1400Plus (120 kV EM used mostly for negative stain purposes) and a JEM-2100F (200 kV EM used for scanning cryo grids). With our setup it is not possible to collect large amounts of high-quality data for reconstructions around 3\u00c5 resolution.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">I also had previous experience with EMBL thanks to a collaboration, and Heidelberg is close by, so EMBL seemed like a good choice.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">How was your experience at EMBL and did you get promising results?<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">The samples I&#8217;m working with are often unevenly distributed on a grid and in the holes. Wim Hagen helped find a solution to manually pick the sample in an efficient way with SerialEM. This allowed us to obtain a dataset with images of the sample and not a majority (80%) of empty ones. If the sample is good, I get the results I need.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Some of the results were published recently:&nbsp;<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Liberta F, Loerch S, Rennegarbe M, <em>et al.<\/em> Cryo-EM fibril structures from systemic AA amyloidosis reveal the species complementarity of pathological amyloids. <em>Nature Communications<\/em> <strong>10<\/strong>: 1104 (2019). <a href=\"https:\/\/doi.org\/10.1038\/s41467-019-09033-z\" target=\"_blank\" rel=\"noreferrer noopener\">DOI: 10.1038\/s41467-019-09033-z<\/a><\/li><li>Radamaker L, <em>et al.<\/em> Cryo-EM structure of a light chain-derived amyloid fibril from a patient with systemic AL amyloidosis. <em>Nature Communications<\/em> <strong>10<\/strong>: 1103 (2019). <a href=\"https:\/\/doi.org\/10.1038\/s41467-019-09032-0\" target=\"_blank\" rel=\"noreferrer noopener\">DOI: 10.1038\/s41467-019-09032-0<\/a><\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">What makes the setup at EMBL\u2019s cryo-EM service platform unique?<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">The flexibility to solve problems and the dedication to getting the best and most data are great.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">If a colleague told you they were considering coming to the EMBL cryo-EM service platform, what would you say?<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Yes, go! You will get high-quality data. I don&#8217;t know a better place for that.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Would you come back?<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Yes, I&#8217;m planning to.<\/p>\n\n<\/div>\n<\/div>\n\n\n<div><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":66,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"embl_taxonomy":[],"class_list":["post-230","page","type-page","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"_links":{"self":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/230","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/comments?post=230"}],"version-history":[{"count":3,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/230\/revisions"}],"predecessor-version":[{"id":326,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/230\/revisions\/326"}],"up":[{"embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/pages\/66"}],"wp:attachment":[{"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/media?parent=230"}],"wp:term":[{"taxonomy":"embl_taxonomy","embeddable":true,"href":"https:\/\/www.embl.org\/about\/info\/imaging-centre\/wp-json\/wp\/v2\/embl_taxonomy?post=230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}