EMBL Imaging Centre

Cutting-edge electron and light microscopy technologies


The publications listed so far are based on the EMBL Cryo-EM Service Platform.


McDowell MA, Heimes M, Fiorentino F, Mehmood S, Farkas Á, Coy-Vergara J, Wu D, Bolla JR, Schmid V, Heinze R, Wild K, Flemming D, Pfeffer S, Schwappach B, Robinson CV, Sinning I. (2020)
Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex
Mol Cell. 2020 Oct 1;80(1):72-86.e7. doi: 10.1016/j.molcel.2020.08.012.

Bansal A, Schmidt M, Rennegarbe M, Haupt C, Liberta F, Stecher S, Puscalau-Girtu I, Biedermann A, Fändrich M. (2021)
AA amyloid fibrils from diseased tissue are structurally different from in vitro formed SAA fibrils
Nat Commun. 2021 Feb 12;12(1):1013. doi: 10.1038/s41467-021-21129-z

Radamaker L, Baur J, Huhn S, Haupt C, Hegenbart U, Schönland S, Bansal A, Schmidt M, Fändrich M. (2021)
Cryo-EM reveals structural breaks in a patient-derived amyloid fibril from systemic AL amyloidosis
Nat Commun. 2021 Feb 8;12(1):875. doi: 10.1038/s41467-021-21126-2

Yu Q, Herrero del Valle A, Singh R, Modis Y. (2021)
MDA5 autoimmune disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA
bioRxiv. doi.org/10.1101/2021.02.01.429133

Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Günnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Münster-Wandowski A, Bürger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT. (2021)
Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit
Mol Cell. 2021 Jan 21;81(2):304-322.e16. doi.org/10.1016/j.molcel.2020.11.037


Kumar A, Planchais C, Fronzes R, Mouquet H, Reyes N (2020)
Binding mechanisms of therapeutic antibodies to human CD20
Science, Vol. 369, Issue 6505, pp. 793-799. doi.org/10.1126/science.abb8008

Ahel J, Lehner A, Vogel A, Schleiffer A, Meinhart A, Haselbach D, Clausen T. (2020)
Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism
eLife. doi.org/10.7554/eLife.56185

Hutchings J, Stancheva VG, Brown NR, Cheung AMC, Miller E, Zanetti G. (2020)
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network
bioRxiv. doi.org/10.1101/2020.06.18.159608

Moser von Filseck J, Barberi L, Talledge  N, Johnson IE, Frost A,  Lenz M,  Roux A. (2020)
Anisotropic ESCRT-III architecture governs helical membrane tube formation
Nat Commun 11, 1516. doi.org/10.1038/s41467-020-15327-4

Bertin A, de Franceschi N, de la Mora E, Maiti S, Alqabandi M, Miguet N, di Cicco  A, Roos W, Mangenot S, Weissenhorn W, Bassereau P. (2020)
Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation
Nat Commun 11, 2663. doi.org/10.1038/s41467-020-16368-5

Flaugnatti N, Rapisarda C , Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot‐Rooke J, Cascales E, Fronzes R, Journet L. (2020)
Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike
The EMBO Journal 39: 39: e104129, doi.org/10.15252/embj.2019104129

Cannac F, Qi C, Falschlunger J,  Hausmann G, Basler K, Korkhov VM. (2020)
Cryo-EM structure of the Hedgehog release protein Dispatched
Science Advances, Vol 6:16, doi.org/10.1126/sciadv.aay7928

Von Kügelgen A, Tang H, Hardy GG, Kureisaite-Ciziene D, Brun YV, Stansfeld PJ, Robinson CV, Bharat TAM (2020)
In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer
Cell. 180 (2): 348-358, doi: https://doi.org/10.1016/j.cell.2019.12.006

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A. (2020)
Structural basis of transcription-translation coupling and collision in bacteria
Science, Vol. 369, Issue 6509, pp. 1355-1359. doi: 10.1126/science.abb5036


Kollmer M, Close W, Funk L, Rasmussen J, Bsoul A, Schierhorn A, Schmidt M, Sigurdson CJ, Jucker M, Fändrich M. (2019)
Cryo-EM structure and polymorphism of Aβamyloid fibrils purified from Alzheimer’s brain tissue
Nat Commun. 10(1):4760. doi: 10.1038/s41467-019-12683-8

Schmidt M, Wiese S, Adak V, Engler J, Agarwal S, Fritz G, Westermark P, Zacharias M, Fändrich M. (2019)
Cryo-EM structure of a transthyretin-derived amyloid fibril from a patient with hereditary ATTR amyloidosis
Nat Commun. 10(1): 5008. doi: 10.1038/s41467-019-13038-z

Abdelkareem M, Saint-André C, Takacs M, Papai G, Crucifix C, Guo X, Ortiz J, Weixlbaumer A. (2019)
Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation
Mol Cell. S1097-2765(19)30321-1 doi: 10.1016/j.molcel.2019.04.029

Qi C, Sorrentino S, Medalia O, Korkhov VM. (2019)
The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein
Science 364(6438): 389-394 doi: 10.1126/science.aav0778

Radamaker L, Lin YH, Annamalai K, Huhn S, Hegenbart U, Schönland SO, Fritz G, Schmidt M, Fändrich M. (2019)
Cryo-EM structure of a light chain-derived amyloid fibril from a patient with systemic AL amyloidosis.
Nat Commun. 10 (1):1103 doi: 10.1038/s41467-019-09032-0

Liberta F, Loerch S, Rennegarbe M, Schierhorn A, Westermark P, Westermark GT, Hazenberg BPC, Grigorieff N, Fändrich M, Schmidt M. (2019)
Cryo-EM fibril structures from systemic AA amyloidosis reveal the species complementarity of pathological amyloids
Nat Commun. 10 (1):1104. doi:10.1038/s41467-019-09033-z

Cuervo A, Fàbrega-Ferrer M, Machón C, Conesa JJ, Fernández FJ, Pérez-Luque R, Pérez-Ruiz M, Pous J, Vega CM, Carrascosa JL, Coll M. (2019)
Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism
Nat Commun. 10: 3746. doi: 10.1038/s41467-019-11705-9

Qi C, Di Minin G, Vercellino I, Wutz A, Korkhov VM (2019)
Structural basis of sterol recognition by human hedgehog receptor PTCH1
Sci Adv. 2019 Sep; 5(9): eaaw6490. doi: 10.1126/sciadv.aaw6490

Desfosses A, Venugopal H, Joshi T, Felix J, Jessop M, Jeong H, Hyun J, Heymann JB, Hurst MRH, Gutsche I, Mitra AK (2019)
Atomic structures of an entire contractile injection system in both the extended and contracted states
Nature Microbiology. doi 10.1038/s41564-019-0530-6

Krupp F, Said N, Huang YH, Loll B, Bürger J, Mielke T, Spahn CMT, Wahl MC. (2019)
Structural Basis for the Action of an All-Purpose Transcription Anti-termination Factor
Molecular Cell, 74:1, 143-157. doi: 10.1016/j.molcel.2019.01.016


Oosterheert W, van Bezouwen LS, Rodenburg RNP, Granneman J, Förster F, Mattevi A, Gros P. (2018)
Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction.
Nat Commun. 9(1):4337. doi: 10.1038/s41467-018-06817-7

Hutchings J, Stancheva V, Miller EA, Zanetti G. (2018)
Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape.
Nat Commun. 9(1):4154. doi: 10.1038/s41467-018-06577-4

Abascal-Palacios G, Ramsay EP, Beuron F, Morris E, Vannini A. (2018)
Structural basis of RNA polymerase III transcription initiation.
Nature 553(7688):301-306. doi: 10.1038/nature25441

Elad, N, Baron S, Peleg Y, Albeck S, Grunwald J, Raviv G, Shakked Z, Zimhony O, Diskin R (2018)
Structure of Type-I Mycobacterium tuberculosis fatty acid synthase at 3.3 Å resolution
Molecular Cell, 74:1. doi: 10.1016/j.molcel.2019.01.016

Böttcher B, Nassal M. (2018)
Structure of Mutant Hepatitis B Core Protein Capsids with Premature Secretion Phenotype
JMB, 430:24. doi: 10.1016/j.jmb.2018.10.018