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EMBL Imaging Centre

Open access to cutting-edge electron and light microscopy

We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology.

Minimal Photon Fluxes (MINFLUX)

If you want to know how MINFLUX works in comparison to other super-resolution technologies we offer – read more here.

MINFLUX

MINFLUX from Abberior Instruments enables imaging of a broad range of biological samples with a spatial resolution in the single nanometer range. This allows to study the structure of large multimolecular protein complexes, such as nuclear pores with unprecedented 3D resolution. 

Additionally, MINFLUX tracking allows to study movement of single molecules in living cells with up to 10 kHz sampling rate (one localisation each 100 µs, which is up to 100 x faster than conventional camera-based tracking). 


Features:

  • MINFLUX imaging with 1-3 nm localisation precision in 2D/3D
  • Tracking of single molecules with up to 10 kHz featuring <20 nm resolution 
  • Nanometre-level active sample stabilization system based on fiducial markers in the sample

Specifications:

  • Laser lines: MINFLUX: 640 nm, 561 nm, Confocal: 488 nm, 405 nm
  • Two-colour ratiometric MINFLUX imaging with 640 nm or 560 nm excitation
  • IX83 inverted microscope stand equipped with a 1.4 NA 100x oil objective
Credit: Sebastian Schnorrenberg/EMBL
U2OS cells: 3D MINFLUX dataset of nuclear pore complex protein Nup96 with Alexa647 labelled SNAP-tag. Shown is the lateral (xy) view of the sample and a sideways (xz) projection of the highlighted subregion. Credit: Sebastian Schnorrenberg/EMBL
Credit: Stuart Bailey/EMBL.
Abberior MINFLUX. Credit: Stuart Bailey/EMBL.

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