{"id":67190,"date":"2022-11-30T16:18:59","date_gmt":"2022-11-30T16:18:59","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/?post_type=vf_event&#038;p=67190"},"modified":"2022-11-30T16:19:02","modified_gmt":"2022-11-30T16:19:02","slug":"neb18-01","status":"publish","type":"vf_event","link":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/events\/neb18-01\/","title":{"rendered":"RNA Sequencing Library Preparation &#8211; How Low Can You Go?"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-53de8397-0f08-449c-b4a4-0fdca47249f2\" data-vf-js-location-nearest-activation-target=\"\">Overview<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-311a4dae-4712-45c4-bb7c-83f569003b70\" data-vf-js-location-nearest-activation-target=\"\">Speakers<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-88113a31-4a51-4c85-a47e-d124b3842495\" data-vf-js-location-nearest-activation-target=\"\">Programme<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-53de8397-0f08-449c-b4a4-0fdca47249f2\"><h2 class=\"vf-u-sr-only\">Overview<\/h2>\n<h2 class=\"wp-block-heading\">Course Overview<\/h2>\n\n\n\n<p>Aim of this course is to prepare RNA-Seq libraries for Illumina sequencing by using the novel NEBNext Ultra II Directional RNA kit for low input applications, not including single cells transcriptomics.<\/p>\n\n\n\n<p>Throughout the course, we will be generating libraries from ultra-low as well as from normal amounts of RNA (1 ng to 50 ng) input to better understand the impact of input amounts and PCR cycles on the bioinformatics dealing with complexity, coverage, duplication rates and uniformity of libraries. The course participants will be guided with tips and tricks through the work flow. Finally, the bioinformatics analysis of the data will be discussed. Selected participants who \u2013 PRIOR to the course &#8211; will be able to prove their samples have required quality according to provided specifications will have the chance to bring maximum two of their own eukaryotic total RNA samples to process. At least 100ng of sample will be required for attendee samples.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Audience<\/h2>\n\n\n\n<p>This course is directed towards wet-lab biologists, scientists and technicians who want to apply transcriptome analysis to \u201ctricky\u201d samples and learn the basis and best practise of library preparation. Knowledge of next generation sequencing technology is desirable but not essential.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Modules\/Resources<\/h2>\n\n\n\n<p>&#8211; Nucleic acid (RNA) processing<br>&#8211; NGS<br>&#8211; Introduction to RNA-Seq data analysis<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Learning Outcomes<\/h2>\n\n\n\n<p>Preparation of libraries from low starting material and dealing with analysing of the data.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-311a4dae-4712-45c4-bb7c-83f569003b70\"><h2 class=\"vf-u-sr-only\">Speakers<\/h2>\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Ascencio_Claudio-1-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Claudio Asencio            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Azevedo_Nayara-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Nayara Azevedo            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Benes_Vladimir-4-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Vladimir Benes            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Burchert_Andreas-.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Andreas Burchert            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Universit\u00e4tsklinik Marburg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Jung_Ferris-1-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Ferris Jung            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Krishnan_Keerthana.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Keerthana Krishnan            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                NEB            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                USA            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Jonathan Landry            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Dinko_Pavlinic.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Dinko Pavlinic            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Jan-Provaznik.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Jan Provaznik            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Bjoern-Textor.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Bj\u00f6rn Textor            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                NEB            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Scientific Organisers<\/h2>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Benes_Vladimir-4-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Vladimir Benes            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Bjoern-Textor.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Bj\u00f6rn Textor            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                NEB            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<h2 class=\"wp-block-heading\">Course Organisers<\/h2>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Maria Mercedes Bacadare Goitia            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n<\/div><\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-88113a31-4a51-4c85-a47e-d124b3842495\"><h2>Programme<\/h2>\n<p id=\"block-1984fb46-4b9e-4350-bb7b-3decc4627750\"><\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-30259d37-b60e-4879-bfe7-15fd0b28110d\" href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/EMBL-Course-RNA-Sequencing-Library-Preparation-How-Low-Can-You-Go_-Programme.pdf\">EMBL-Course-RNA-Sequencing-Library-Preparation-How-Low-Can-You-Go_-Programme<\/a><\/div>\n\n\n\n<p><\/p>\n<\/section>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"parent":0,"menu_order":0,"template":"","embl_taxonomy":[],"class_list":["post-67190","vf_event","type-vf_event","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"vf_event_start_date":{"value":"20180320","formatted":"20 March 2018"},"vf_event_end_date":{"value":"20180323","formatted":"23 March 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"},"vf_event_start_time":{"value":"","formatted":""},"vf_event_end_time":{"value":"","formatted":""},"vf_event_location":{"value":["67"],"formatted":["EMBL Heidelberg"]},"vf_event_time_zone":{"value":"europe\/berlin","formatted":"Europe\/Berlin"},"vf_event_ss_subtype":{"value":null,"formatted":null},"vf_event_embo_subtype":{"value":"","formatted":""},"vf_event_featured":{"value":"0","formatted":false},"vf_event_organisers_listing":{"value":"","formatted":""},"vf_event_displayed":{"value":"EMBL Course","formatted":"EMBL 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