{"id":46956,"date":"2022-07-15T13:46:09","date_gmt":"2022-07-15T12:46:09","guid":{"rendered":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/?post_type=vf_event&#038;p=46956"},"modified":"2024-02-22T12:51:29","modified_gmt":"2024-02-22T12:51:29","slug":"gee23-01","status":"publish","type":"vf_event","link":"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/events\/gee23-01\/","title":{"rendered":"Genome engineering: CRISPR\/Cas from cells to mice"},"content":{"rendered":"\n<div class=\"vf-tabs\"><ul class=\"vf-tabs__list\" data-vf-js-tabs=\"true\"><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-overview\" data-vf-js-location-nearest-activation-target=\"\">Overview<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-ab6531ef-da92-4054-b956-d30882627647\" data-vf-js-location-nearest-activation-target=\"\">Speakers<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-programme\" data-vf-js-location-nearest-activation-target=\"\">Programme<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-information\" data-vf-js-location-nearest-activation-target=\"\">Practical Information<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-sponsors\" data-vf-js-location-nearest-activation-target=\"\">Sponsors<\/a><\/li><li class=\"vf-tabs__item\"><a class=\"vf-tabs__link\" href=\"#vf-tabs__section-mediakit\" data-vf-js-location-nearest-activation-target=\"\">Media kit<\/a><\/li><\/ul><div class=\"vf-tabs-content\" data-vf-js-tabs-content=\"true\">\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-overview\"><h2 class=\"vf-u-sr-only\">Overview<\/h2>\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Course overview<\/h2>\n\n\n\n<p>This course will provide training in genome editing and cell engineering in mammalian cells and mouse embryos using the highly efficient CRISPR\/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knock-outs\/knock-ins, and target validation using the most current technologies. The course will be split into two groups (mammalian cells&nbsp;<strong>OR<\/strong>&nbsp;mice) based on participants&#8217; expertise.<\/p>\n\n\n\n<p>Please note that all participants are expected to engage in all sessions. Participants should plan for approximately 1 hour extra per day for self paced learning modules.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Audience<\/h2>\n\n\n\n<p>This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and who want to learn how to create an engineered mammalian cell line or mouse model using the most recent and advanced CRISPR\/Cas9 system. No previous experience in genome editing is required.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Modules\/resources<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>How to create a CRISPR\/Cas9 experiment using various design tools<\/li>\n\n\n\n<li>Transfection optimisation for efficient gene editing<\/li>\n\n\n\n<li>Generation of knock-out and knock-in cell lines and mouse embryos<\/li>\n\n\n\n<li>Validation of genome-edited cell lines and embryos<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Learning outcomes<\/h2>\n\n\n\n<p>After this course you should be able to: <\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Design a CRISPR based project using editing tools for your target gene of interest<\/li>\n\n\n\n<li>Choose the right format of gene editing tool and delivery strategy for your cell type or embryos<\/li>\n\n\n\n<li>Edit, screen and validate the engineered models, embryo manipulation<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">What past participants say about the course<\/h2>\n\n\n\n<p><em>\u201cThe EMBL course about CRISPR applications for genome engineering was an incredible experience. From the quality of the lectures, the availability of the invited speakers to respond to all questions and doubts, the openness and diversity of participants to the great organization and the impressive facilities and core facility teams working there. It all created a perfect atmosphere for learning about this groundbreaking technique and the inspiring work being done with it as well as great networking. I highly recommend this course.\u201d<\/em>&nbsp;\u2013 Sofia Morazzo, International Clinical Research Center, Czech Republic<\/p>\n\n\n\n<p>&#8220;<em>The course covers all you need to organise your CRISPR experiments in great detail. The speakers are fantastic and eager to help with your specific questions. The organisation is also fantastic, where you only have to think on CRISPR and nothing else.<\/em>&#8221; \u2013 Sergio G. San Miguel, Max Planck Institute for Infection Biology, Germany<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>Please see EMBL\u2019s&nbsp;<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/covid-19-safety-policy\/\">COVID-19 safety policy<\/a>&nbsp;for testing and other requirements to attend this on-site course.<\/p>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-ab6531ef-da92-4054-b956-d30882627647\"><h2>Speakers<\/h2>\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<p><\/p>\n\n\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Adamson_Antony.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/sites.manchester.ac.uk\/genome-editing-unit\/\" target=\"_self\" class=\"vf-profile__link\">Antony Adamson<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                The University of Manchester            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                UK            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/10\/Ascolani_Michela.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Michela Ascolani            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Rome            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Italy            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Alessandro Barenghi            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL  Rome            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Italy            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"258\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Carroll_Dana-258x300.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Carroll_Dana-258x300.jpg 258w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Carroll_Dana-882x1024.jpg 882w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Carroll_Dana-768x892.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Carroll_Dana.jpg 1102w\" sizes=\"auto, (max-width: 258px) 100vw, 258px\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/medicine.utah.edu\/faculty\/mddetail\/u0028835\" target=\"_self\" class=\"vf-profile__link\">Dana Carroll<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                University of Utah School of Medicine            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                USA            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Deckert_Silke-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Silke Deckert            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Thermo Fisher Scientific            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Julian Gr\u00fcnewald            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Technical University of Munich            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/10\/Kaulich_Manuel.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"\" target=\"_self\" class=\"vf-profile__link\">Manuel Kaulich<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Universit\u00e4tsklinikum Frankfurt            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Frank Montoya            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.embl.org\/people\/person\/bneumann\/\" target=\"_self\" class=\"vf-profile__link\">Beate Neumann<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Paix.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Alexandre Paix            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Remans_Kim-1-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.embl.org\/groups\/protein-expression-purification\/\" target=\"_self\" class=\"vf-profile__link\">Kim Remans<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Isabel Rollan            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/CP-60daniel-schraivogel.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Daniel Schraivogel            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Maj Simonsen Jackson            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Rome            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Italy            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n            <img decoding=\"async\" class=\"vf-profile__image\" src=\"https:\/\/dev.assets.emblstatic.net\/vf\/v2.0.0-alpha.6\/assets\/vf-summary\/assets\/vf-icon--avatar.svg\" > \n    \n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Kieran Van Kessel            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Thermo Fisher Scientific            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                UK            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Scientific Organisers<\/h2>\n\n\n\n<p><\/p>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/Humphreys_Neil.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.embl.org\/groups\/gene-editing-and-embryology-facility\/\" target=\"_self\" class=\"vf-profile__link\">Neil Humphreys<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Rome            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Italy            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"225\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Kristof-Kersse-225x300.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Kristof-Kersse-225x300.jpg 225w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Kristof-Kersse-768x1024.jpg 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Kristof-Kersse-1152x1536.jpg 1152w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Kristof-Kersse-1536x2048.jpg 1536w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Kristof-Kersse-scaled.jpg 1920w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.thermofisher.com\/de\/en\/home\/life-science\/genome-editing.html\" target=\"_self\" class=\"vf-profile__link\">Kristof Kersse<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Thermo Fisher Scientific            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Koch_Birgit-3.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.mr.mpg.de\/person\/54979\/13990930\" target=\"_self\" class=\"vf-profile__link\">Birgit Koch<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Max Planck Institute for Medical Research            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"115\" height=\"135\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Zielonka_Elisabeth-1-1.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    <a href=\"https:\/\/www.embl.org\/people\/person\/elisabeth-zielonka\/\" target=\"_self\" class=\"vf-profile__link\">Elisabeth Zielonka<\/a>\n            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Course Organisers<\/h2>\n\n\n\n<div class=\"embl-grid\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/2021\/03\/chris-stocks.jpg\" class=\"vf-profile__image\" alt=\"Chris Stocks\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Chris Stocks            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Course and Conference Team Lead            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<article class=\"vf-profile vf-profile--very-easy vf-profile--medium vf-profile--block | vf-u-margin__bottom--400\">\n\n    <img decoding=\"async\" width=\"155\" height=\"175\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/CP-60039236-2.jpg\" class=\"vf-profile__image\" alt=\"\" loading=\"lazy\" itemprop=\"image\" \/>\n    <h3 class=\"vf-profile__title\" style=\"text-align: center;\">\n                    Swetlana Krez            <\/h3>\n    \n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Training Lab             <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                EMBL Heidelberg            <\/p>\n                <p class=\"vf-profile__text\" style=\"text-align: center;\">\n                Germany            <\/p>\n    \n    \n      \n\n    \n<\/article>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-programme\"><h2>Programme<\/h2>\n<p id=\"block-1984fb46-4b9e-4350-bb7b-3decc4627750\">Got something to say? Tweet it! <strong>#EMBLCRISPRcas<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Some pre-course work (such as watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.<\/li>\n\n\n\n<li>Some speakers may need to join virtually to give their lecture or Q&amp;A, and the programme is subject to change.<\/li>\n<\/ul>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 1 &#8211; Sunday 17 September 2023<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th>Time  <br>(Europe\/Berlin) <\/th><th>                     Session<\/th><th>Location <\/th><\/tr><\/thead><tbody><tr><td>16:00 &#8211; 16:30                                                                        <\/td><td><strong>Registration <\/strong><\/td><td>ISG Hotel<\/td><\/tr><tr><td>16:30 &#8211; 17:30<\/td><td><strong>Arrival &amp; Welcome<\/strong><br>Introduction to course design <\/td><td>ISG Seminar room                                       <\/td><\/tr><tr><td>17:30 &#8211; 18:00<\/td><td><strong>Icebreaker<\/strong><\/td><td>ISG Seminar room     <\/td><\/tr><tr><td>18:00 &#8211; 19:00<\/td><td><strong>Live Keynote Lecture:<br>Introduction to CRISPR\/Cas 9<\/strong><br>Dana Carroll &#8211; University of Utah School of Medicine,  USA<br>**this talk will be remote**<\/td><td>ISG Seminar room <\/td><\/tr><tr><td>19:00<\/td><td><strong>Dinner &amp; Networking <\/strong><\/td><td>ISG Hotel  <\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 2 &#8211; Monday 18 September 2023<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th>Time (Europe\/Berlin) <\/th><th class=\"has-text-align-left\" data-align=\"left\">                             Session <\/th><th> Location<\/th><\/tr><\/thead><tbody><tr><td>08:30 &#8211; 08:45                                                       <\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Bus from ISG Hotel to EMBL<\/strong><\/td><td> ISG Hotel<\/td><\/tr><tr><td>08:45 &#8211; 09:00                      <\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Overview of the day<\/strong><\/td><td>Courtyard Room<\/td><\/tr><tr><td>09:00- 12:15<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Day 2: Guide RNA Design<\/strong><\/td><td><\/td><\/tr><tr><td>09:00- 09:15<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Lab Safety<\/strong><br>Swetlana Kretz &#8211; EMBL Heidelberg, Germany<\/td><td>Courtyard Room<\/td><\/tr><tr><td>09:15 &#8211;  10:15<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 1: gRNA\/Donor design <br><\/strong>Kristof Kersse  &#8211; Thermo Fisher Scientific, Germany<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>10:15 &#8211;  11:15<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 1: gRNA\/Donor design<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>11:15 &#8211; 11:45<\/td><td class=\"has-text-align-left\" data-align=\"left\">Coffee <\/td><td><\/td><\/tr><tr><td>11:45 &#8211; 12:30 <\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical in groups<\/strong><\/td><td><\/td><\/tr><tr><td>11:45 &#8211; 12:30<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Mammalian Cell Group<\/strong><br><strong>Practical 1.1: TrueTag Donor Template Synthesis \u2013 PCR<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kristof Kersse &#8211;  Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK<\/td><td>Training Lab A <\/td><\/tr><tr><td>11:45 &#8211; 12:30<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Mouse Embryo Group<\/strong><br><strong>Glass transfer pipet prep, harvest embryos and short seminar on embryo transfer and demonstration.<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy<br>Alessandro Barenghi \u2013 EMBL Rome, Italy<br>Isabel Rollan &#8211; EMBL Heidelberg, Germany<br>Frank Montoya &#8211; EMBL Heidelberg, Germany<\/td><td>Training Lab B<\/td><\/tr><tr><td>12:30- 13:30<\/td><td class=\"has-text-align-left\" data-align=\"left\">Lunch <\/td><td><\/td><\/tr><tr><td>13:30 &#8211; 14:30<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Lecture: A rationale for efficient genome editing using linear repair donors<\/strong><br>Alexandre Paix  &#8211; EMBL Heidelberg, Germany<\/td><td>Courtyard Room<\/td><\/tr><tr><td>14:30 &#8211; 16:00<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 1.2<\/strong><br><strong>Mammalian Cell Group: TrueTag Donor Template Synthesis &#8211; Gel analysis and Clean-up<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kristof Kersse &#8211;  Thermo Fisher Scientific, Germany <br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK<\/td><td>Training Lab  A<\/td><\/tr><tr><td>14:30 &#8211; 16:00<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 1.2<br>Mouse Embryo Group:<\/strong> <strong>Intro to TLR2 system &amp; describe injection mix prep- microinjection zygotes  &amp; electroporation.<\/strong><br>Electroporation:<br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy <br>Michela Ascolani \u2013 EMBL Rome, Italy Alessandro Barenghi \u2013 EMBL Rome, Italy<br><br>Microinjection:<br>Neil Humphreys \u2013 EMBL Rome, Italy Isabel Rollan \u2013 EMBL Heidelberg, Germany <br>Frank Diego Montoya Castillo- EMBL Heidelberg, Germany <br>Sandra Stobrawa \u2013 Biozol\/Calibre Scientific<\/td><td>Training Lab  B<\/td><\/tr><tr><td>16:00- 16:30<\/td><td class=\"has-text-align-left\" data-align=\"left\">Coffee <\/td><td><\/td><\/tr><tr><td>16:30 &#8211; 18:00<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 2 +3<\/strong><br><strong>Mammalian Cell Group<\/strong><br><strong>Group A: gRNA efficiency: Lipid transfection of 293FT cells with gRNA and Cas9 protein,<\/strong> <br><strong>Lipid transfection of 293FT-eBFP cells with gRNA, Cas9 protein and ssODN<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK<br><br><strong>Group B:Guide RNA design task<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br>Kristof Kersse &#8211; Thermo Fisher Scientific, Germany<\/td><td>Cell Culture &amp; Flex Lab<\/td><\/tr><tr><td>16:30 &#8211; 18:00<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Mouse Embryo Group:<\/strong><br><strong>2nd Microinjection, electroporation and AAV<\/strong><br>Electroporation:<br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy <br>Michela Ascolani \u2013 EMBL Rome, Italy Alessandro Barenghi \u2013 EMBL Rome, Italy<br><br>Microinjection:<br>Neil Humphreys \u2013 EMBL Rome, Italy Isabel Rollan \u2013 EMBL Heidelberg, Germany <br>Frank Diego Montoya Castillo- EMBL Heidelberg, Germany <br>Sandra Stobrawa \u2013 Biozol\/Calibre Scientific<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>18:00- 19:00<\/td><td class=\"has-text-align-left\" data-align=\"left\">Dinner <\/td><td><\/td><\/tr><tr><td>19:00- 20:30<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 2 +3<\/strong><br><strong>Mammalian Cell Group<br>Group B: gRNA efficiency: Lipid transfection of 293FT cells with gRNA and Cas9 protein,<br>Lipid transfection of 293FT-eBFP cells with gRNA, Cas9 protein and ssODN<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK <br><br><strong>Group A:Guide RNA design task<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br>Kristof Kersse &#8211; Thermo Fisher Scientific, Germany<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>19:00- 20:30<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Practical 2+3<\/strong><br><strong>Mouse Embryo Group<br>Extra time with Embryos microinjection and embryos transfer KSOM media O\/N culture<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy<br>Alessandro Barenghi \u2013 EMBL Rome, Italy<br>Isabel Rollan &#8211; EMBL Heidelberg, Germany<br>Frank Montoya &#8211; EMBL Heidelberg, Germany<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>20:30<\/td><td class=\"has-text-align-left\" data-align=\"left\"><strong>Bus from EMBL to ISG Hotel<\/strong><\/td><td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 3 &#8211; Tuesday 19 September 2023<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th>Time<br>(Europe\/Berlin) <\/th><th>                          Session<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>08:30 &#8211; 08:45                                                <\/td><td><strong>Bus from ISG Hotel to EMBL<\/strong><\/td><td><\/td><\/tr><tr><td>08:45 &#8211;  09:15<\/td><td><strong><strong>Overview of the day<\/strong><\/strong><\/td><td><\/td><\/tr><tr><td>09:15 &#8211; 10:15<\/td><td><strong>Lecture<\/strong>: <strong>Gene editing in different experimental models<\/strong><br>Antony Adamson &#8211; The University of Manchester, UK<\/td><td>Courtyard Room<\/td><\/tr><tr><td>10:15 &#8211; 11:15<\/td><td><strong>Practical Lecture<\/strong> <br><strong>Mammalian Cell Group: Generation and validation of endogenously tagged cell line<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany <\/td><td>Courtyard Room<\/td><\/tr><tr><td>10:15 &#8211; 11:15<\/td><td><strong>Practical 4.  Mouse Embryo Group: Assess and clean-up 2 cell microinjected embryos<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<br>Isabel Rollan &#8211; EMBL Heidelberg, Germany <br>Frank Montoya &#8211; EMBL Heidelberg, Germany<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>11:15- 11:45<\/td><td><strong>Coffee<\/strong><\/td><td><\/td><\/tr><tr><td>11:45 &#8211; 12:45<\/td><td><strong>Lecture:<\/strong> <strong>CRISPR Prime Editing<\/strong><br>Julian Gr\u00fcnewald, Technical University of Munich, Germany<\/td><td>Courtyard Room<\/td><\/tr><tr><td>12:45 &#8211; 13:45<\/td><td><strong>Lunch <\/strong><\/td><td><\/td><\/tr><tr><td>13:45 &#8211; 15:45<\/td><td><strong>Practical 1 &#8211; 3 + 4<\/strong><br><strong>Mammalian Cell Group<\/strong><br><strong>Group A: Tag Knock-in: Electroporation of 293FT with gRNA, Cas9 protein and TrueTag donor template<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK <br><br><strong>Group B: Project discussion<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br>Kristof Kersse &#8211; Thermo Fisher Scientific, Germany<\/td><td>Cell Culture\/Flex Lab<br><br><br><br><br><br><br><br><br><br><br><br>Courtyard Room<\/td><\/tr><tr><td>13:45 &#8211; 15:45<\/td><td><strong>Practical 4<br>Mouse Embryo Group<br>Thaw pre-prepared blastocysts, assess viability and extract DNA<\/strong><br>Neil Humphreys \u2013 EMBL Rome, Italy Michela Ascolani \u2013 EMBL Rome, Italy <br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy Isabel Rollan \u2013 EMBL Heidelberg Alessandro Barenghi \u2013 EMBL Rome, Italy<\/td><td>Training Lab A &amp; B <\/td><\/tr><tr><td>15:45- 16: 15<\/td><td><strong>Coffee <\/strong><\/td><td><\/td><\/tr><tr><td>16:15- 18:15<\/td><td><strong>Practical 1 &#8211; 3 + 4<br>Mammalian Cell Group<br>Group B: Tag Knock-in: Electroporation of 293FT with gRNA, Cas9 protein and TrueTag donor template<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK <br><strong>Group A: Project discussion<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br>Kristof Kersse &#8211; Thermo Fisher Scientific, Germany<\/td><td>Cell Culture\/Flex Lab<\/td><\/tr><tr><td>16:15- 18:15<\/td><td><strong>Practical 5<br>Mouse Embryo Group<br>Set up T7 assay and mutant-specific PCRs<\/strong><br>Neil Humphreys \u2013 EMBL Rome, Italy Michela Ascolani \u2013 EMBL Rome, Italy Maj Simonsen Jackson \u2013 EMBL Rome, Italy Alessandro Barenghi \u2013 EMBL Rome, Italy<\/td><td> Training Lab A &amp; B<\/td><\/tr><tr><td>18:15 &#8211; 19:15<\/td><td><strong>Dinner<\/strong><\/td><td>Canteen<\/td><\/tr><tr><td>19:45<\/td><td><strong>Bus from EMBL to ISG hotel<\/strong><\/td><td>EMBL Bus stop<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 4 &#8211; Wednesday 20 September 2023<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th>Time (Europe\/Berlin) <\/th><th>                            Session<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>08:30 &#8211; 08:45                        <\/td><td><strong>Bus from ISG Hotel to EMBL<\/strong><\/td><td>&nbsp;<\/td><\/tr><tr><td>08:45 &#8211; 09:15<\/td><td><strong><strong>Overview of the day<\/strong><\/strong><\/td><td>Courtyard Room<\/td><\/tr><tr><td>09:15 &#8211; 10:15<\/td><td><strong>Lecture: <\/strong>Daniel Schraivogel<br>EMBL Heidelberg, Germany<\/td><td>Courtyard Room<\/td><\/tr><tr><td>10:15- 12:15<\/td><td><strong>Practical in Groups<\/strong><br><strong>Mammalian Cell Group Practicals 5-1 &amp; 5-2:<br>GCD Cell Harvest and lysis, set up GCD PCR<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kristof Kersse &#8211; Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK<\/td><td>Training Lab  B <\/td><\/tr><tr><td>10:15- 12:15<\/td><td><strong>Mouse Embryo Group: Practicals 5.1 &amp; 5.22<br>Assess and clean-up 4 to 8 cell embryos. Run Gels for PCRs<br>Set up T7 assay and digestion assay PCRs.<\/strong><br>Neil Humphreys \u2013 EMBL Rome, Italy Michela Ascolani \u2013 EMBL Rome, Italy <br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy<br>Alessandro Barenghi \u2013 EMBL Rome, Italy Isabel Rollan \u2013 EMBL Heidelberg, Germany<\/td><td>Training Lab A <\/td><\/tr><tr><td>12:30- 13:15<\/td><td><strong>Lunch&nbsp;<\/strong><\/td><td> <\/td><\/tr><tr><td>13:15 &#8211; 13:30<\/td><td><strong>Group photo<\/strong><\/td><td><\/td><\/tr><tr><td>13:30- 14:30<\/td><td><strong>Lecture:  The 3Cs technology for CRISPR gRNA library generation<\/strong><br>Manuel Kaulich- Goethe University, Germany<\/td><td>Courtyard Room<\/td><\/tr><tr><td>14:30 &#8211; 16:30<\/td><td><strong>Practical in Groups<br>Practical 5.3: Mammalian Cell Group<\/strong><br><strong>CR analysis, set up GCD re-annealing reaction<br><br>Practical 5.4 &amp; 5.5<\/strong><br><strong>Set up digestion for GCD, gel purification &amp; sample submission for sanger sequencing<br>Gel analysis of GCD<\/strong><br>Silke Deckert-  Thermo Fisher Scientific, Germany<br>Kristof Kersse &#8211; Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK<\/td><td>Flex Lab A &amp; B <\/td><\/tr><tr><td>14:30 &#8211; 16:30<\/td><td><strong>Mouse Embryo Group:<br>Project discussion, focus on the analysis of sequencing data and design options<\/strong><br>Neil Humphreys \u2013 EMBL Rome, Italy <br>Michela Ascolani \u2013 EMBL Rome, Italy Alessandro Barenghi \u2013 EMBL Rome, Italy Isabel Rollan \u2013 EMBL Heidelberg, Germany Antony Adamson \u2013 The University of Manchester, UK<\/td><td>Training Lab A &amp; B<\/td><\/tr><tr><td>16:30- 17:00<\/td><td><strong>Coffee&nbsp;<\/strong><\/td><td><\/td><\/tr><tr><td>17:00- 18:00<\/td><td><strong>Lecture: Imaged-based high-throughput screening RNAi vs CRISPR<\/strong><br>Beate Neumann, EMBL Heidelberg, Germany&nbsp;<\/td><td>Courtyard Room<\/td><\/tr><tr><td>18:00- 20:30<\/td><td><strong>Pizza Dinner and Discussion round and drinks:<\/strong> Ethical issues of CRISPR technology and genome engineering<\/td><td>Roof Top Lounge<\/td><\/tr><tr><td>20:30<\/td><td> Bus from EMBL to ISG hotel <\/td><td>EMBL bus stop<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 5 &#8211; Thursday 21 September 2023<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th>Time <br>(Europe\/Berlin) <\/th><th>                      Session<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>08:30 &#8211; 08:45                                                    <\/td><td><strong>Bus from ISG Hotel to EMBL<\/strong>           <\/td><td>&nbsp;<\/td><\/tr><tr><td>08:45 &#8211; 09:15<\/td><td><strong><strong>Overview of the day<\/strong><\/strong><\/td><td>&nbsp;<\/td><\/tr><tr><td>09:15 &#8211; 10:45<\/td><td><strong>Practical in Groups<br>Mammalian Cell Group<br>Group A: Examine BFP cells via microscopy and FACS<\/strong><br>Kristof Kersse &#8211;  Thermo Fisher Scientific, Germany<br>Kieran Van Kessel  &#8211; Thermo Fisher Scientific, UK  <br>FACS Specialist, Thermo Fisher<br><strong>Group B: Knock-In cell validation &#8211; DNA Preparation<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany             <\/td><td>Training Lab  B<\/td><\/tr><tr><td>09:15 &#8211; 10:45<\/td><td><strong>Mouse Embryo Group<br>Thaw TLR2 embryos and examine KO and KI morular\/ blastocysts for florescence<br>Knock-In validation.<\/strong><br>Neil Humphreys \u2013 EMBL Rome, Italy Michela Ascolani \u2013 EMBL Rome, Italy <br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy Isabel Rollan \u2013 EMBL Heidelberg, Germany Beate Neumann &#8211; EMBL Heidelberg, Germany<\/td><td>Training Lab B<\/td><\/tr><tr><td>10:45 &#8211; 11:15<\/td><td><strong>Coffee&nbsp;<\/strong><\/td><td> <\/td><\/tr><tr><td>11:15 &#8211; 12:45<\/td><td><strong>Practical in Groups<br>Mammalian Cell Group<br><\/strong><strong>Knock-In cell validation &#8211; FACS Analysis<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<\/td><td>Cell Culture\/116\/Training Lab B<\/td><\/tr><tr><td>11:15 &#8211; 12:45<\/td><td><strong>Mouse Embryo Group<\/strong><br><strong>Image embryos<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<\/td><td>ALMF Facility<\/td><\/tr><tr><td>12:45 &#8211; 13:45<\/td><td><strong>Lunch&nbsp;<\/strong><\/td><td><\/td><\/tr><tr><td>13:45 &#8211; 15:00<\/td><td><strong>Practical in groups<\/strong><br><strong>Mammalian cell group<br>Knock-In cell validation \u2013 Set up Junction PCR<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<\/td><td>Training Lab  B <\/td><\/tr><tr><td> 13:45 &#8211; 15:00 <\/td><td><strong>Mouse embryo group<\/strong><br>Tour of LAR Facility                            <\/td><td><\/td><\/tr><tr><td>15:00 &#8211; 15:30<\/td><td><strong>Break<\/strong><\/td><td>&nbsp;<\/td><\/tr><tr><td>15:30 &#8211; 16:30<\/td><td><strong>Lecture: CRISPR-Cas genome editing to generate transgenic mice<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<\/td><td>Courtyard Room<\/td><\/tr><tr><td>16:30 &#8211; 18:30<\/td><td><strong>Technology Lounge:<\/strong> <strong>Genome modification technologies<br>Presentation by different companies<\/strong>.  <\/td><td><\/td><\/tr><tr><td>18:45<\/td><td><strong>Bus from EMBL to downtown (via ISG Hotel)<\/strong><\/td><td>&nbsp;ATC entrance<\/td><\/tr><tr><td>19:00<\/td><td><strong>Downtown course dinner<\/strong><\/td><td>Oskars Downtown<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nDay 6 &#8211; Friday 22 September 2023<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<figure class=\"wp-block-table is-style-regular\"><table><thead><tr><th>Time (Europe\/Berlin) <\/th><th>                                 Session<\/th><th>Location<\/th><\/tr><\/thead><tbody><tr><td>08:30 &#8211; 08:45                      <\/td><td><strong>Bus from ISG Hotel to EMBL<\/strong><\/td><td>&nbsp;ISG Hotel<\/td><\/tr><tr><td>08:45 &#8211; 09:15<\/td><td><strong><strong>Overview of the day<\/strong><\/strong><\/td><td>Courtyard Room<\/td><\/tr><tr><td>09:15 &#8211; 10:15<\/td><td><strong>Lecture: <\/strong>Purification of Cas9 protein<br>Kim Remans- EMBL Heidelberg, Germany<\/td><td>Courtyard Room<\/td><\/tr><tr><td>10:15-11:15<\/td><td><strong>Practical in Groups<br>Mammalian Cell Group<\/strong><br><strong>Gel electrophoresis of junction PCR<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br><\/td><td>Training Lab  B<\/td><\/tr><tr><td>10:15-11:15<\/td><td><strong>Mouse Embryo Group<\/strong><br><strong>Project discussion<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy<br>Alessandro Barenghi \u2013 EMBL Rome, Italy<br>Isabel Rollan &#8211; EMBL Heidelberg, Germany<br>Frank Montoya &#8211; EMBL Heidelberg, Germany<\/td><td>Flex Lab A &amp; B<\/td><\/tr><tr><td>11:15- 11:45<\/td><td><strong>Break<\/strong><\/td><td><\/td><\/tr><tr><td>11:45- 12:45<\/td><td><strong>Practical in Groups<br>Mammalian Cell Group<\/strong><br><strong>Analysis of gel electrophoresis<\/strong><br>Birgit Koch &#8211; Max Planck Institute for Medical Research, Germany<br><\/td><td>Training Lab  B<\/td><\/tr><tr><td>11:45- 12:45<\/td><td><strong>Mouse Embryo Group<\/strong><br><strong>Project discussion<\/strong><br>Neil Humphreys &#8211; EMBL Rome, Italy<br>Michela Ascolani &#8211; EMBL Rome, Italy<br>Maj Simonsen Jackson \u2013 EMBL Rome, Italy<br>Alessandro Barenghi \u2013 EMBL Rome, Italy<br>Isabel Rollan &#8211; EMBL Heidelberg, Germany<br>Frank Montoya &#8211; EMBL Heidelberg, Germany<\/td><td>Flex Lab A &amp; B<\/td><\/tr><tr><td>12:45- 13:45<\/td><td><strong>Lunch<\/strong><\/td><td><\/td><\/tr><tr><td>13:45- 15:00<\/td><td><strong>The Solution Room- Last chance to ask questions<\/strong><\/td><td> Flex Lab A &amp; B  <\/td><\/tr><tr><td>15:00- 15:30<\/td><td><strong>Final discussions, feedback session<\/strong><\/td><td> Flex Lab A &amp; B  <\/td><\/tr><\/tbody><\/table><\/figure>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-information\"><h2>Practical Information<\/h2>\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nRegistration fees and motivation letter<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p>The course is&nbsp;<strong>limited to 20 participants<\/strong>. For selection purposes, please note that your application will not be considered without a&nbsp;letter of motivation.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Registration<\/h2>\n\n\n\n<p><strong>Registration fees<\/strong>&nbsp;include admission, course materials, COVID-19 safety measures, meals and coffee breaks.  Participants are expected to book and pay their own accommodation and travel expenses.&nbsp;<br><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>Academia<\/strong><\/td><td>\u20ac790<\/td><\/tr><tr><td><strong>PhD Student<\/strong><\/td><td>\u20ac790<\/td><\/tr><tr><td><strong>Industry<\/strong><\/td><td>\u20ac1200<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>NO visa support letters<\/strong>&nbsp;will be issued until payment of the registration fee is confirmed.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Confirmation and payment<\/h2>\n\n\n\n<p>The registration fee should be paid only after acceptance to the course.&nbsp;The results will be announced approximately 2-3 weeks after the application deadline.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"letter\">Motivation letter submission<\/h2>\n\n\n\n<p>After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering. <\/p>\n\n\n\n<p><strong>Instructions<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Include your relevant skills, experience and qualifications showing why you would be suitable for the course.<\/li>\n\n\n\n<li>Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.<\/li>\n<\/ol>\n\n\n\n<p>Please note: <\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox). <\/li>\n\n\n\n<li>If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text. <\/li>\n\n\n\n<li>If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised. <\/li>\n<\/ul>\n\n\n\n<p>For detailed instructions, please watch our <a href=\"https:\/\/youtu.be\/M5OHYbAc4mA\">video on how to submit a course motivation letter<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Further details<\/h2>\n\n\n\n<p>For further information about registration and motivation letter submission please refer to the&nbsp;<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/frequently-asked-questions\/\">FAQ page<\/a>.<\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nFinancial assistance<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\">Financial assistance<\/h2>\n\n\n\n<p>Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both&nbsp;<strong>registration fee waivers<\/strong>&nbsp;and&nbsp;<strong>travel grants<\/strong>.&nbsp;Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the abstract or motivation letter submission process.<\/p>\n\n\n\n<p>Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Registration fee waiver<\/h3>\n\n\n\n<p>The&nbsp;<strong>fee waiver<\/strong>&nbsp;will cover the registration sum that you have paid to attend the course or conference.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Travel grant<\/h3>\n\n\n\n<p>The&nbsp;<strong>travel grant<\/strong>&nbsp;will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and\/or registration fees*) and is provided up to specified caps which are normally as follows:<\/p>\n\n\n\n<p>&#8211; up to \u20ac400 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from within Europe.<br>&#8211; up to \u20ac1000 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from&nbsp;outside Europe.<br>&#8211; up to \u20ac500 for any participant travelling to an EMBO Practical Course or EMBO Workshop.<br>&#8211; up to \u20ac1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course or EMBO Workshop.<\/p>\n\n\n\n<p>*Registration fees are only covered for EMBO Practical Courses or EMBO Workshops<br><br>The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Childcare grant (EMBO practical courses and workshops only)<\/h3>\n\n\n\n<p>For EMBO Practical Course or EMBO Workshop participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course or conference. Eligible costs include fees for a babysitter or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc.&nbsp;Please note that priority will be given to early-stage researchers. A maximum amount of 500 EUR can be awarded per participant applying for an EMBO Childcare Grant.  In order to apply for this grant for EMBO Workshops, you must be registered by the abstract submission deadline.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Boehringer ingelheim fonds travel grant<\/h3>\n\n\n\n<p>Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.<\/p>\n\n\n\n<p>This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.<br>Whether you are eligible to apply for a travel grant,&nbsp;depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):<\/p>\n\n\n\n<p>&#8211; for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course<br>&#8211; for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Application<\/strong><\/h2>\n\n\n\n<p>You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.<\/p>\n\n\n\n<p>For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Selection<\/strong><\/h2>\n\n\n\n<p>The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Reimbursement<\/strong><\/h2>\n\n\n\n<p>Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Further details<\/strong><\/h2>\n\n\n\n<p>See our <a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/external-funding-sources\/\">list of external funding opportunities<\/a> and <a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/become-an-event-reporter\/\">information on attending a conference as an event reporter<\/a>.<\/p>\n\n\n\n<p>For further information about financial assistance please refer to the&nbsp;<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/frequently-asked-questions\/\">FAQ page<\/a>.<\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nAccommodation and travel<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<h2 class=\"wp-block-heading\">Travel information<\/h2>\n\n\n\n<p>For travel information,<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/travel-information\/\"> please see here<\/a>.<\/p>\n\n\n\n<p>If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn \u2018Event Ticket\u2019 (called the \u2018Veranstaltungsticket\u2019 in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket. <\/p>\n\n\n\n<p>For more information<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Event-ticket-details.pdf\"> in English see here<\/a> or<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Details-zum-Event-ticket.pdf\"> in German see here<\/a>. <\/p>\n\n\n\n<p>You can <a href=\"https:\/\/www.veranstaltungsticket-bahn.de\/?event=8462&amp;language=en\">book your ticket here<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Hotel<\/h2>\n\n\n\n<p>Single rooms have been reserved for 5 nights (17 &#8211; 21 September 2023) at the <a href=\"https:\/\/isg-hotel.de\/en\/\" target=\"_blank\" rel=\"noreferrer noopener\">ISG Hotel<\/a>. After the selection process, selected participants should contact the hotel directly to make a booking (<strong>booking code GEE23-01<\/strong>).  We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.  <\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Local transport<\/h2>\n\n\n\n<p>Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Further details<\/h2>\n\n\n\n<p>Address: EMBL Heidelberg, Meyerhofstra\u00dfe 1, 69117 Heidelberg, Germany<\/p>\n\n\n\n<p>For more information about accommodation and travel, please refer to the <a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/frequently-asked-questions\/#FAQ_travel\">FAQ page<\/a><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/information-for-participants\/frequently-asked-questions\/\">.<\/a><\/p>\n\n<\/div>\n<\/details>\n\n\n\n<details  class=\"vf-details\" id=\"\"  >\n<summary class=\"vf-details--summary\">\nEvent software<\/summary>\n<div class=\"acf-innerblocks-container\">\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Event software<\/h2>\n\n\n\n<p>The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.&nbsp;<br><\/p>\n\n<\/div>\n<\/details>\n<\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-sponsors\"><h2>Sponsors<\/h2>\n<p><strong>Event Partner<\/strong><\/p>\n\n\n\n<div class=\"vf-grid | vf-grid__col-2\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/www.thermofisher.com\/de\/de\/home.html\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/thermo-fisher-logo-480x270-copy2-7.jpg\" alt=\"\" class=\"wp-image-120316\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/thermo-fisher-logo-480x270-copy2-7.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/thermo-fisher-logo-480x270-copy2-7-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-1\"><\/div>\n\n\n\n<p><strong>Event Sponsor<\/strong><\/p>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/www.biozol.de\/zellmanipulation\/\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/BIOZOL_LOGO_EMBL-1.jpg\" alt=\"\" class=\"wp-image-123382\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/BIOZOL_LOGO_EMBL-1.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/BIOZOL_LOGO_EMBL-1-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/www.olympus-lifescience.com\/en\/landing\/olympus-microscopes\/?creative=484334759901&amp;keyword=microscopy&amp;matchtype=p&amp;network=g&amp;device=c&amp;campaignid=223054447&amp;adgroupid=15724981087&amp;gclid=Cj0KCQjw2eilBhCCARIsAG0Pf8vkvzDMSBiZb5smzHafa37sHdbwm6kpECcsjFLJ37hay_ln61Yj3P0aAtE_EALw_wcB\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Evident-new_website_480X270-1.jpg\" alt=\"\" class=\"wp-image-123386\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Evident-new_website_480X270-1.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Evident-new_website_480X270-1-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/eu.idtdna.com\/pages\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/IDT-logo-480x270-copy2-2.jpg\" alt=\"\" class=\"wp-image-123388\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/IDT-logo-480x270-copy2-2.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/IDT-logo-480x270-copy2-2-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/international.neb.com\/\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/neb-logo-480x270-copy-4.jpg\" alt=\"\" class=\"wp-image-123390\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/neb-logo-480x270-copy-4.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/neb-logo-480x270-copy-4-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"http:\/\/www.xceltis.de\/\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/xceltis_website_480X270-1.jpg\" alt=\"\" class=\"wp-image-123392\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/xceltis_website_480X270-1.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/xceltis_website_480X270-1-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Event Supporter<\/strong><\/p>\n\n\n\n<div class=\"vf-grid | vf-grid__col-4\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/www.coopersurgical.com\/\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/coopersurgical_website_480X270-1.jpg\" alt=\"\" class=\"wp-image-120306\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/coopersurgical_website_480X270-1.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/coopersurgical_website_480X270-1-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-full\"><a href=\"https:\/\/www.vitrolife.com\/\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"270\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Vitrolife_website_480X270-1.jpg\" alt=\"\" class=\"wp-image-120308\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Vitrolife_website_480X270-1.jpg 480w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/Vitrolife_website_480X270-1-300x169.jpg 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><\/a><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p><strong>Media partners<\/strong><\/p>\n\n\n\n<p><em><a href=\"https:\/\/ojs.bonviewpress.com\/index.php\/MEDIN\/index\" data-type=\"URL\" data-id=\"https:\/\/ojs.bonviewpress.com\/index.php\/MEDIN\/index\">Medinformatics<\/a><\/em>, a Gold Open Access journal<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>Sponsorship opportunities<\/strong><\/p>\n\n\n\n<p>We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget.&nbsp;Discounts are available for companies sponsoring multiple events at EMBL Heidelberg.&nbsp;<a href=\"https:\/\/www.embl.org\/events\/\">View other events<\/a>, or contact&nbsp;<a href=\"mailto:sponsorship@embl.de\">sponsorship@embl.de<\/a>&nbsp;for further information.<\/p>\n\n\n\n<p>If you are interested in becoming a media partner of this event, please visit our&nbsp;<a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/sponsor-an-event\/#vf-tabs__section-08208670-4ad4-45ca-a4ff-ac6edb6ffd0b\" target=\"_blank\" rel=\"noreferrer noopener\">media partnerships webpage<\/a>.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><p><a href=\"mailto:sponsorship@embl-em.de?subject=%5BIn%20situ%20structural%20biology%5D%20Sponsorship%20Brochure&#038;body=Please%20kindly%20send%20me%20the%20sponsorship%20brochure%20for%20EMBO%20Workshop:%20In%20situ%20structural%20biology:%20from%20cryo-EM%20to%20multi-scale%20modelling%20(8%20-%2011%20February%202023).\" class=\"vf-button vf-button--primary vf-button--pill\">Request a sponsorship brochure<\/a><\/p><\/div>\n\n\n\n<div class=\"vf-box vf-box--normal vf-box-theme--quaternary | vf-u-margin__bottom--400\">\n      <h3 class=\"vf-box__heading\">\n                Warning                  <\/h3> \n        <p class=\"vf-box__text\">EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name \u2018Judy Eastman\u2019 (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL\u2019s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.<\/p>\n<p class=\"vf-box__text\">Please also note that:<\/p>\n<ul>\n<li><span style=\"font-size: 12pt;\">EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.<\/span><\/li>\n<li><span style=\"font-size: 12pt;\">EMBL will never call or email you to ask for your credit card details or to request a payment.<\/span><\/li>\n<li><span style=\"font-size: 12pt;\">All payments are on invoice.<\/span><\/li>\n<\/ul>\n<p class=\"vf-box__text\">Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.<\/p>\n<\/div><\/section>\n\n\n\n<section class=\"vf-tabs__section\" id=\"vf-tabs__section-mediakit\"><h2>Media kit<\/h2>\n<p>Want to let others know you&#8217;re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.<\/p>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<div class=\"wp-block-media-text alignwide is-stacked-on-mobile\"><figure class=\"wp-block-media-text__media\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story.png\"><img loading=\"lazy\" decoding=\"async\" width=\"169\" height=\"300\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story-169x300.png\" alt=\"\" class=\"wp-image-107462 size-medium\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story-169x300.png 169w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story-576x1024.png 576w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story-768x1365.png 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story-864x1536.png 864w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_IG-story.png 1080w\" sizes=\"auto, (max-width: 169px) 100vw, 169px\" \/><\/a><\/figure><div class=\"wp-block-media-text__content\">\n<p><\/p>\n<\/div><\/div>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large is-style-default\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1024\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD-1024x1024.png\" alt=\"\" class=\"wp-image-107466\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD-1024x1024.png 1024w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD-300x300.png 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD-150x150.png 150w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD-768x768.png 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01-AD.png 1080w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\">Social 1080&#215;1080<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook-1024x576.png\" alt=\"\" class=\"wp-image-107464\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook-1024x576.png 1024w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook-300x169.png 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook-768x432.png 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook-1536x864.png 1536w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_Twitter-Facebook.png 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\">Social 1600&#215;900<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"vf-grid | vf-grid__col-3\"><div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<figure class=\"vf-figure wp-block-image size-large\"><a href=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" class=\"vf-figure__image\" src=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide-1024x576.png\" alt=\"\" class=\"wp-image-107468\" srcset=\"https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide-1024x576.png 1024w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide-300x169.png 300w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide-768x432.png 768w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide-1536x864.png 1536w, https:\/\/www.embl.org\/about\/info\/course-and-conference-office\/wp-content\/uploads\/GEE23-01_title_slide.png 1920w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"vf-figure__caption\">PPT Slide 16&#215;9<\/figcaption><\/figure>\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n\n\n<div class=\"\"><!--[vf\/content]-->\n<div class=\"vf-content\">\n\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\"><\/div><\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"parent":0,"menu_order":0,"template":"","embl_taxonomy":[],"class_list":["post-46956","vf_event","type-vf_event","status-publish","hentry"],"acf":[],"embl_taxonomy_terms":[],"vf_event_start_date":{"value":"20230917","formatted":"17 September 2023"},"vf_event_end_date":{"value":"20230922","formatted":"22 September 2023"},"vf_event_submission_opening":{"value":"","formatted":""},"vf_event_submission_closing":{"value":"","formatted":""},"vf_event_registration_opening":{"value":"","formatted":""},"vf_event_registration_closing":{"value":"","formatted":""},"vf_event_summary":{"value":"","formatted":""},"vf_event_additional_info":{"value":"","formatted":""},"vf_event_registration_fee":{"value":"","formatted":""},"vf_event_canceled":{"value":"no","formatted":"no"},"vf_event_venue":{"value":"EMBL Advanced Training Centre","formatted":"EMBL Advanced Training 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11:20:48","modified":"2022-10-06 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